Post‐viraemic detection of bovine ephemeral fever virus by use of autogenous lymphoid tissue‐derived bovine primary cell cultures

Abstract

BEFV has preferential tropism for bovine lymphoid tissues and the spleen and haemal node may be potential sites for post-viraemic virus replication.

In this issue – January/February 2017

“…We have previously published a paper from this group that discussed the epidemiology of this virus . In the current paper they discuss the localisation of antigens in various tissues, as well as its presence within cell cultures, from a case that was confirmed using qRT‐PCR …”

“…Middelton et al make the point that identification of the antigen is not regarded as sufficient evidence that a particular tissue is the site of virus replication, as this may simply reflect sequestration of virus or fragments in phagocytes or cellular debris . However, Barigye et al are more confident that their positive staining does demonstrate virus survival and replication in macrophages …”

Bovine Ephemeral Fever (BEF) : Penyebab, Epidemiologi, Diagnosa, dan Terapi

“…Diagnosa BEF dengan ELISA pernah dilakukan oleh Balai Besar Penelitian Veteriner untuk membedakana dengan penyakit lain terutama Peste des petits ruminants (PPR), Rinderpest, Bluetongue, dan Pasteurellosis (Sendow et al, 2017). Uji diagnostik molekuler yang dapat digunakan untuk diagnosa BEF antara lain adalah imunohistokimia, PCR, RT-PCR, real-time RT-qPCR (Bakhshesh and Abdollahi, 2015;Zaghawa et al, 2017;Barigye et al, 2017). Sedangkan uji diagnostik molekuler yang telah dikembangkan untuk mendeteksi BEF adalah metode Real-time PCR/RT-PCR dan reverse transcription loopmediated isothermal amplification/RTLAMP dan kedua metode tersebut sangat sensitif terhadap gen G yang menjadi targetnya Zheng et al, 2011).…”