Postembedding immunohistochemical demonstration of antigen in experimental polyarthritis using plastic embedded whole joints

Hermanns, W.; Liebig, Katrin; Schulz, L. Cl.

doi:10.1007/bf00495658

Cited by 57 publications

(26 citation statements)

References 14 publications

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“…Samples were fixed in paraformaldehyde (4%) for 24 to 48 hours and subsequently embedded in paraffin and plastic. 24 Sections of 5 mm and 2 mm were routinely stained with HE, according to Giemsa and periodic acid-Schiff (PAS), reaction and examined by light microscopy. Selected skin sections of SA 6, SA 20, and SA 22 taken during subacute and chronic stages were also stained with Masson's trichrome stain and picrosirius red/Alcian blue.…”

Section: Skin Sample Collection and Tissue Preparationmentioning

confidence: 99%

Naturally Acquired Bovine Besnoitiosis

Langenmayer¹,

Gollnick²,

Majzoub-Altweck³

et al. 2014

Vet Pathol

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The pathogenesis of bovine besnoitiosis, a disease of increasing concern within Europe, is still incompletely understood. In this study, disease progression after natural infection with the causative apicomplexan Besnoitia besnoiti was monitored in histological skin sections of 5 individual female cattle over time. High-frequency skin sampling of 2 cattle with mild and 2 with severe acute, subacute, and chronic besnoitiosis, as well as from 1 animal during subclinical disease, enabled documentation from the beginning of the disease. Skin sections were stained with hematoxylin and eosin and Giemsa, periodic acid-Schiff reaction, and anti-Besnoitia immunohistochemistry. In all 4 clinically affected animals, tachyzoite-like endozoites could be detected for the first time by immunohistochemistry, and tissue cyst evolution was monitored. Besnoitiosis-associated lesions were not detected in the animal showing the subclinical course. Because of the inconsistency of the nomenclature of Besnoitia tissue cyst layers in the literature, a new nomenclature for B. besnoiti cyst wall layers is proposed: tissue cysts consist of a hypertrophied host cell with enlarged nuclei, an intracytoplasmic parasitophorous vacuole with bradyzoites, a sometimes vacuolated inner cyst wall, and an outer cyst wall in more developed cysts. Inner and outer cyst walls can be readily distinguished by using special stains. In 1 animal, extracystic B. besnoiti zoites were immunohistochemically detected during the chronic stage. At necropsy, the 2 severely affected cows displayed large numbers of B. besnoiti cysts in a variety of tissues, including the corium of the claws, contributing mainly to the development of chronic laminitis in these 2 cases.

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Section: Skin Sample Collection and Tissue Preparationmentioning

confidence: 99%

Naturally Acquired Bovine Besnoitiosis

Langenmayer¹,

Gollnick²,

Majzoub-Altweck³

et al. 2014

Vet Pathol

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“…For scientific interest, a ~2 cm 3 specimen was excised from the periphery of the white, multilobulated tumour and fixed in 10% formalin for histopathological evaluation. Sections of paraffin-embedded samples and of glycolmethacrylate/methylmethacrylate-embedded samples (Hermanns et al 1981) were routinely prepared and stained with haematoxylin and eosin (HE) and reticulin preparation. Immunostaining of cytokeratin, vimentin, desmin, actin, calponin, MAC387 and lysozyme was performed using horseradish peroxidase-coupled secondary antibodies r,s .…”

Section: Case Descriptionmentioning

confidence: 99%

Hemangiopericytoma in a cat: a case report

Blutke¹,

Knebel²,

Brühschwein³

et al. 2012

Vet. Med.

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ABSTRACT:In dogs and men, hemangiopericytoma is a well-recognised neoplasm, thought to originate from vascular pericytes. In cats, however, hemangiopericytoma is an extremely rare finding. The present report describes the pathological features of a 7-cm-diameter, dense, white, multilobulated tumour-like growth on the thigh of a ten-year-old Persian cat. Histologically, the mass consisted of polygonal neoplastic cells, concentrically arranged around thin-walled endothelium-lined blood vessels occasionally forming typical staghorn-configurations. In reticulin-stained sections, a dense meshwork of argyrophilic collagen fibres was evident, surrounding the central vessels and separating individual tumour cells. Within the tumour tissue, there were areas of extensive necrosis and degeneration. In the periphery, the tumour displayed a moderate infiltrative growth into the adjacent musculature. Immunohistochemical analysis revealed strong diffuse cytoplasmatic immunoreactivity for vimentin, a focal immunoreactivity for S-100, and a weak interstitial staining for laminin, whereas neoplastic cells stained negative for cytokeratin, desmin, actin, calponin, von Willebrand factor, glial fibrillary acidic protein, neuron specific enolase, CD79a, MAC387, lysozyme and MHCII. Ultrastructurally, the neoplastic cells contained few intracytoplasmatic filaments, and were surrounded by interlacing bundles of intercellular long-spacing collagen fibres. Occasionally, desmosome-like intermediate junctions were observed between neighbouring tumour cells. On the basis of histomorphology, ultrastructure and immunohistochemical reactivity, the neoplasm was diagnosed as a hemangiopericytoma, representing the second reported case of this rare tumour entity in a cat.

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“…Cytology (Diff-Quick staining kit, Fisher Scientific GmbH, Schwerte, Germany) of an impression smear showed a moderate number of mostly degenerated neutrophils, plasma cells and clusters of polygonal epithelial cells. For histopathological examination, specimens of the excised mass were fixed in 10 per cent buffered formaldehyde and routinely embedded in paraffin and glycolmethacrylate/methylmethacrylate (Hermanns et al, 1981). Histologically, the mass was composed of a fibrous connective tissue stroma with decent, accentuated subepithelial infiltration of neutrophils, macrophages, lymphocytes and plasma cells, and few foci of calcification and cholesterol clefts.…”

Section: Case Descriptionmentioning

confidence: 99%

Inflammatory polyp in the middle ear of a dog: a case report

Blutke¹,

Parzefall²,

Steger³

et al. 2010

Vet. Med. - Czech

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Nasopharyngeal polyps are non-neoplastic masses, originating from the mucosa of the nasopharynx, the tympanic bulla or the Eustachian tube. Inflammatory polyps extending into the tympanic bulla cavity are a common cause of otitis media in cats. In dogs, however, occurrence of middle ear polyps has rarely been reported. The present report describes the findings of the clinical examination, diagnostic imaging and histopathological appraisal of a ten year old male dog with an inflammatory middle ear polyp arising from the mucosa of the Eustachian tube. Clinically, the dog displayed a peripheral vestibular syndrome. Magnetic resonance imaging revealed a hyperintense soft tissue mass filling the right middle ear cavity. Following ventral bulla osteotomy, a polypoid growth with a stalk arising from the auditory tube was surgically excised from the tympanic bulla. Histologically, the polyp was composed of a fibrous connective tissue stroma with discreet infiltration of inflammatory cells and an overlying surface layer of partially ulcerated respiratory epithelium. Similarities and differences between the histological appearance of the present case and the few previously reported records of canine middle ear polyps are discussed, along with a comparative review of etiological, pathogenetic and therapeutic aspects of middle ear polyps in cats and dogs

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Postembedding immunohistochemical demonstration of antigen in experimental polyarthritis using plastic embedded whole joints

Cited by 57 publications

References 14 publications

Naturally Acquired Bovine Besnoitiosis

Naturally Acquired Bovine Besnoitiosis

Hemangiopericytoma in a cat: a case report

Inflammatory polyp in the middle ear of a dog: a case report

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