Interest in producing specialty melons (Cucumis melo) is increasing in Florida, but information on yield performance, fruit quality, and disease resistance of specialty melon cultivars grown in Florida conditions is limited. In this study conducted at Citra, FL, during the 2011 Spring season, 10 specialty melon cultivars were evaluated, in both certified organic and conventionally managed fields, including: Creme de la Creme and San Juan ananas melon (C. melo var. reticulatus), Brilliant and Camposol canary melon (C. melo var. inodorus), Ginkaku and Sun Jewel asian melon (C. melo var. makuwa), Arava and Diplomat galia melon (C. melo var. reticulatus), and Honey Pearl and Honey Yellow honeydew melon (C. melo var. inodorus). ‘Athena’ cantaloupe (C. melo var. reticulatus) was included as a control. ‘Sun Jewel’, ‘Diplomat’, ‘Honey Yellow’, and ‘Honey Pearl’ were early maturing cultivars that were harvested 10 days earlier than ‘Athena’. ‘Athena’ had the highest marketable yield in the conventional field (10.7 kg/plant), but the yield of ‘Camposol’, ‘Ginkaku’, ‘Honey Yellow’, and ‘Honey Pearl’ did not differ significantly from ‘Athena’. Under organic production, ‘Camposol’ showed a significantly higher marketable yield (8.3 kg/plant) than ‘Athena’ (6.8 kg/plant). ‘Ginkaku’ produced the largest fruit number per plant in both organic (10 fruit/plant) and conventional fields (12 fruit/plant) with smaller fruit size compared with other melon cultivars. Overall, the specialty melon cultivars, except for asian melon, did not differ significantly from ‘Athena’ in terms of marketable fruit number per plant. ‘Sun Jewel’, ‘Diplomat’, and ‘San Juan’ showed relatively high percentages of cull fruit. ‘Honey Yellow’, ‘Honey Pearl’, and ‘Sun Jewel’ exhibited higher soluble solids concentration (SSC) than ‘Athena’ in both organic and conventional fields, while ‘Brilliant’, ‘San Juan’, and ‘Ginkaku’ also had higher SSC than ‘Athena’ under organic production. ‘Honey Yellow’, ‘Sun Jewel’, ‘Brilliant’, and ‘Camposol’ were less affected by powdery mildew (caused by Podosphaera xanthii) and downy mildew (caused by Pseudoperonospora cubensis) in the conventional field. ‘Honey Yellow’ and ‘Camposol’ also had significantly lower aboveground disease severity ratings in the organic field compared with ‘Athena’, although the root-knot nematode (RKN) (Meloidogyne sp.) gall rating was higher in ‘Honey Yellow’ than ‘Athena’.