Background Green chilies are grown in tropical and subtropical regions globally. Pakistan is the main stakeholder and plays a vital role in chili’s export. However, approximately 15% of the country's chili yield goes to waste due to inadequate post-harvest handling. The deterioration of natural antioxidants during storage is a primary reason for reduced chili shelf-life and quality. While cold storage helps extend market life, its use is limited for temperature-sensitive produce like green chilies. In this regard, an experiment was conducted to evaluate the impact of alone or combined application of hot water treatment (HWT) and eucalyptus leaf extract (ELE) on 'Golden Hot' chilies in comparison to control. After treatment, chilies were stored at 20 ± 1.5°C for 20 days.Results HWT + ELE treated chilies exhibited significant improvements compared to the control, with significant reductions in fungal decay, red chilies, weight loss, soluble solids, ripening index, and reactive oxygen species. HWT + ELE also enhanced antioxidative properties i.e., DPPH scavenging activities, ascorbic acid, phenolics, and enzyme activities. Moreover, HWT + ELE-treated chilies displayed improved chlorophyll levels and overall appearance, with reduced anthocyanins, and wrinkling, resulting in increased marketable fruits compared to the control.Conclusion The pre-storage HWT and ELE application offers an effective treatment for preserving green chilies at ambient temperature.