Postheparin Plasma Diamine Oxidase in Patients With Small Bowel Crohn's Disease

D’Agostino, Luciano; Daniele, Bruno; Pallone, Francesco; Pignata, S.; Leoni, Marco; Mazzacca, G

doi:10.1016/s0016-5085(88)80069-6

Cited by 31 publications

(12 citation statements)

References 23 publications

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“…Common parameters for this evaluation are too unspecific and reflect a more general participation of the intestine. Histological examination often fails in classification and differentiation for the diagnosis of Crohn's disease, As a better and earlier parameter plasma diamine oxidase released from the intestine after heparin injection was suggested [5,6]. This enzyme is mainly located in the mucosal cells of the small and large bowel from where it is released.…”

Section: Discussionmentioning

confidence: 99%

“…The determination of functional abnormalities of mucosa with regard to permeability of sugars is a further measurement of inflammatory activity in Crohn's disease [4]. Recent studies recommended the assay of plasma diamine oxidase (DAO) activity after heparin injection as an unambiguous and early parameter for assessment of activity of inflammation in Crohn's disease [5,6]. DAO is the key-enzyme for the metabolism of diamines in man [7], which has its highest activity in the intestinal mucosa and is localized in the cytoplasm of the mature enterocytes of the small and large bowel [8].…”

Section: Introductionmentioning

confidence: 99%

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Human intestinal diamine oxidase (DAO) activity in Crohn's disease: A new marker for disease assessment?

et al. 1990

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The key-enzyme for the metabolism of diamines in man is diamine oxidase (DAO). Its highest activities are in the intestinal mucosa, localized in the cytoplasm of the mature enterocytes of the small and large bowel. If the gut is affected by inflammation in Crohn's disease macroscopical changes are observed. This prospective study investigated if these mucosal alterations are also reflected in changes of mucosal diamine oxidase activity and/or mucosal histamine content respectively. Twenty patients (12 female, 8 male; age: means = 31, range 18-49 years) undergoing gut resection because of complications in Crohn's disease (Jan.-Dec. 1988) formed the basis of the study. Tissue samples of the resected material from areas inflamed and histologically not involved in the disease were investigated for diamine oxidase activities and histamine content. Diamine oxidase activities in the mucosa obtained from the macroscopically normal proximal (155.6; (76-393) mU/g (means, range)) and distal (132; (58.5-295) mU/g) resection margins were similar to our previous findings. In all patients, however, samples from the diseased mucosa had significantly (ca. 50%) lower diamine oxidase activities (74.5; (5-262) mU/g) compared to the healthy tissue. Similar differences were found in material obtained either from whole intestinal wall or from the mucosa. The determination of diamine oxidase activity constitutes possibly a more unambiguous and earlier parameter for assessing the extent of the inflamed area than histological disease presentations. Using biopsies the necessary extent of resection could be estimated before operation: this may influence operative strategies and help in the definition of the minimum amount of inflamed gut to be removed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human intestinal diamine oxidase (DAO) activity in Crohn's disease: A new marker for disease assessment?

et al. 1990

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“…Altered levels of the intestinal enzyme diamine oxidase have been reported in CD (8,9). Under normal conditions plasma levels are very low, but they are increased to measurable levels following an intravenous bolus of heparin, which releases diamine oxidase from its binding sites (10).…”

Section: Introductionmentioning

confidence: 99%

Intestinal permeability and postheparin plasma diamine oxidase activity in the prediction of Crohn's disease relapse

et al. 2007

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i Summary: A method of detecting presymptomatic relapse of Crohn's disease could allow for the selective use of maintenance or intensified medical therapy in those with an increased risk of relapse. The aim of this study was to evaluate three potential laboratory markers of relapse: intestinal and gastroduodenal permeability and plasma diamine oxidase activity. Intestinal permeability (lactulose/mannitol test), gastroduodenal permeability (urinary sucrose excretion), and postheparin plasma diamine oxidase activity were serially measured in 61 adults with Crohn's disease in remission (CDAI < 150) for at least 30 days. Subjects were followed periodically for clinical relapse (CDAI > 150 and increased by at least 100 points or the

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“…In a brief report [66], Bayless et al also found low unstimulated plasma DAO activity in pa tients with Crohn's disease. However, our observation that the post-heparin AUC re sults correlated, inversely, with the Crohn's disease activity index (CDAI) has been con firmed recently [67], Furthermore, when these patients underwent remission of their disease, the CDAIs fell and the low AUCs again returned towards normal [3], The rea son why active ileal Crohn's disease should depress post-heparin plasma (and presum ably also ileal mucosal) DAO activity is un known.…”

Section: Small Bowel Resectionmentioning

confidence: 87%

Polyamines in Intestinal Adaptation and Disease

Dowling¹

1990

Digestion

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Postheparin Plasma Diamine Oxidase in Patients With Small Bowel Crohn's Disease

Cited by 31 publications

References 23 publications

Human intestinal diamine oxidase (DAO) activity in Crohn's disease: A new marker for disease assessment?

Human intestinal diamine oxidase (DAO) activity in Crohn's disease: A new marker for disease assessment?

Intestinal permeability and postheparin plasma diamine oxidase activity in the prediction of Crohn's disease relapse

Polyamines in Intestinal Adaptation and Disease

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