Postmortem corneal changes evaluated by fluorescent staining

Dogaroiu, Catalin; Zarojanu, Andrei; Scurtu, R; Morosanu, Gabriel; Tătaru, Călin Petru; Cristina, Anca

doi:10.4323/rjlm.2014.13

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…After scoring was completed for each region, the scores were totaled. It is important that the same person who don’t know the grouping situation performed and scored each operation [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

SPARC overexpression in allogeneic adipose-derived mesenchymal stem cells in dog dry eye model induced by benzalkonium chloride

Li,

Han

et al. 2024

Stem Cell Res Ther

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Background Nowadays, companion and working dogs hold significant social and economic importance. Dry eye, also known as dry keratoconjunctivitis (KCS), a common disease in ophthalmology, can readily impact a dog’s working capacity and lead to economic losses. Although there are several medications available for this disease, all of them only improve the symptoms on the surface of the eye, and they are irritating and not easy to use for long periods of time. Adipose-derived mesenchymal stem cells (ADMSC) are promising candidates for tissue regeneration and disease treatment. However, long-term in vitro passaging leads to stemness loss of ADMSC. Here, we aimed to use ADMSC overexpressing Secreted Protein Acidic and Rich in Cysteine (SPARC) to treat 0.25% benzalkonium chloride-treated dogs with dry eye to verify its efficacy. For in vitro validation, we induced corneal epithelial cell (HCECs) damage using 1 µg/mL benzalkonium chloride. Methods Fifteen male crossbred dogs were randomly divided into five groups: normal, dry eye self-healing control, cyclosporine-treated, ADMSC-CMV-treated and ADMSC-OESPARC-treated. HCECs were divided into four groups: normal control group, untreated model group, ADMSC-CMV supernatant culture group and ADMSC-OESRARC supernatant culture group. Results SPARC-modified ADMSC had the most significant effect on canine ocular surface inflammation, corneal injury, and tear recovery, and the addition of ADMSC-OESPARC cell supernatant also had a salvage effect on HCECs cellular damage, such as cell viability and cell proliferation ability. Moreover, analysis of the co-transcriptome sequencing data showed that SPARC could promote corneal epithelial cell repair by enhancing the in vitro viability, migration and proliferation and immunosuppression of ADMSC. Conclusion The in vitro cell test and in vivo model totally suggest that the combination of SPARC and ADMSC has a promising future in novel dry eye therapy. Graphical abstract

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Section: Methodsmentioning

confidence: 99%

SPARC overexpression in allogeneic adipose-derived mesenchymal stem cells in dog dry eye model induced by benzalkonium chloride

Li,

Han

et al. 2024

Stem Cell Res Ther

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“…Prieto-Bonete and Luna proposed a histological analysis to detect morphological lens changes for postmortem interval estimation [58]. Dogariou's group evaluated corneal postmortem changes using fluorescent staining [59].…”

Section: State Of the Artmentioning

confidence: 99%

Postmortem Ocular Findings in the Optical Coherence Tomography Era: A Proof of Concept Study Based on Six Forensic Cases

et al. 2021

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Postmortem analysis of the ocular globe is an important topic for forensic pathology and transplantology. Although crucial elements may be gathered from examining cadaveric eyes, the latter do not routinely undergo in-depth analysis. The paucity of quantitative and objective data that are obtainable using current, invasive necroscopic techniques is the main reason for the limited interest in this highly specialized procedure. The aim of the current study is to describe and to object for the first time the postmortem ocular changes by mean of portable optical coherence tomography for evaluating ocular tissues postmortem. The design involved the postmortem analysis (in situ, and without enucleation) of 12 eyes by portable spectral-domain Optical Coherence Tomography. The scans were performed, in corneal, retinal and angle modality at different intervals: <6 h, 6th, 12th, and 24th hour and after autopsy (25th–72nd hour). The morphological changes in the cornea, sclera, vitreous humor and aqueous humor were easy to explore and objectify in these tissues in first 72 h postmortem. On the other hand, the “in situ” observation of the retina was difficult due to the opacification of the lenses in the first 24 h after death.

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Morphological analysis of corneal findings modifications after death: A preliminary OCT study on an animal model

Nioi

Napoli

Demontis

et al. 2018

Experimental Eye Research

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Postmortem corneal changes evaluated by fluorescent staining

Cited by 3 publications

References 15 publications

SPARC overexpression in allogeneic adipose-derived mesenchymal stem cells in dog dry eye model induced by benzalkonium chloride

SPARC overexpression in allogeneic adipose-derived mesenchymal stem cells in dog dry eye model induced by benzalkonium chloride

Postmortem Ocular Findings in the Optical Coherence Tomography Era: A Proof of Concept Study Based on Six Forensic Cases

Morphological analysis of corneal findings modifications after death: A preliminary OCT study on an animal model

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