Postnatal developmental profile of 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthetase and cholesterol 7α-hydroxylase activities in the liver of domestic swine

Kwekkeboom, Jaap; Kempen, H.J.M.; Voorthuizen, E.M. van; Griffioen, Marieke; Cohen, Louis

doi:10.1016/0005-2760(90)90070-e

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…CYP7A1, an enzyme generally considered to be rate-limiting in bile acid biosynthesis (1), increased several-fold with age both at the enzyme activity level and the mRNA level. The developmental pattern found for CYP7A1 is partly in agreement with previously reported findings (7,8). Kwekkeboom et al (7) also reported an increase of cholesterol 7␣-hydroxylase activity with age but found the highest activity in 4 to 8-week-old female pigs.…”

Section: Cyp7a1supporting

confidence: 91%

“…Kwekkeboom et al (7) also reported an increase of cholesterol 7␣-hydroxylase activity with age but found the highest activity in 4 to 8-week-old female pigs. In rats, cholesterol 7␣-hydroxylase is reported to be low in newborns but increased at weaning (7,42).…”

Section: Cyp7a1mentioning

confidence: 94%

“…Although a limited amount of data are available on developmental changes of CYP7A1 (7)(8)(9), the variation during different periods of life of the bile acid forming enzymes is largely unknown. A controlled study of the development of these enzymes in humans is hampered by the difficulty in obtaining tissue material from individuals of different ages.…”

mentioning

confidence: 99%

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Expression of key enzymes in bile acid biosynthesis during development: CYP7B1-mediated activities show tissue-specific differences

Norlin

2002

Journal of Lipid Research

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The developmental variation of cytochrome P450 (CYP)7A1, CYP7B1, CYP27A1, and 3 ␤ -hydroxy-⌬ 5 -C 27 -steroid dehydrogenase, key enzymes in bile acid biosynthesis, were investigated in pigs of different ages. As part of these studies, peptide sequences from a purified pig liver oxysterol 7 ␣ -hydroxylase were analyzed. The sequences showed a high degree of identity with those of murine and human CYP7B1. Enzymatic activities and mRNA levels of CYP27A1 and 3 ␤ -hydroxy-⌬ 5 -C 27 -steroid dehydrogenase were similar in livers of newborn and 6-month-old pigs. Enzymatic activity mediated by CYP7A1 increased several-fold between infancy and adolescence. Hepatic CYP7A1 and CYP7B1 mRNA levels increased several-fold with age. Hepatic microsomal 7 ␣ -hydroxylation of 27-hydroxycholesterol and dehydroepiandrosterone, substrates typical for CYP7B1, increased about 5-fold between infancy and adolescence whereas the activities in kidney microsomes decreased at least 10-fold. In conclusion, the results indicate that the expression of CYP27A1 and 3 ␤ -hydroxy-⌬ 5 -C 27 -steroid dehydrogenase are similar in livers of newborn and 6-month-old pigs whereas the levels of CYP7A1 increase. The finding that the levels of CYP7B1 increase with age in the liver but decrease in the kidney suggest a tissue-specific developmental regulation of CYP7B1. The age-dependent variation in the liver and kidney suggests that hormonal factors are involved in the regulation of CYP7B1. -Norlin, M. Expression of key enzymes in bile acid biosynthesis during development: CYP7B1-mediated activities show tissue-specific differences.

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Section: Cyp7a1supporting

confidence: 91%

Section: Cyp7a1mentioning

confidence: 94%

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Expression of key enzymes in bile acid biosynthesis during development: CYP7B1-mediated activities show tissue-specific differences

Norlin

2002

Journal of Lipid Research

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Hepatic cholesterol and bile acid synthesis in Japanese patients with cholesterol gallstones

et al. 1993

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In Japan the composition of gallstones is changing rapidly from the once-predominant brown-pigment stones to cholesterol ones. The present work was undertaken to clarify the mechanism of cholesterol supersaturated bile production in Japanese patients with cholesterol gallstones. In 26 non-obese and normolipidemic patients (11 with cholesterol gallstones, 8 with black- or brown-pigment gallstones, 7 without gallstones) a liver biopsy and hepatic bile were surgically obtained under standardized conditions. The cholesterol saturation of hepatic bile was significantly higher in cholesterol gallstone patients than in gallstone-free controls (195 +/- 10 vs. 146 +/- 8%, respectively; P < 0.01). The microsomal activities of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme for cholesterol synthesis, cholesterol 7 alpha-hydroxylase, the rate-limiting enzyme for bile acid synthesis, and 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase (12 alpha-hydroxylase), the rate-limiting enzyme for cholic acid synthesis, were assayed simultaneously in the same subjects. There were positive correlations between HMG-CoA reductase and cholesterol 7 alpha-hydroxylase activities (Rs = 0.62, P < 0.005), and between cholesterol 7 alpha-hydroxylase and 12 alpha-hydroxylase activities (Rs = 0.44, P < 0.05) in all subjects, irrespective of the existence of gallstones. The activities of the three rate-limiting enzymes did not differ significantly among the three groups (cholesterol stone, pigment stone and stone-free). In conclusion, the cholesterol supersaturation of hepatic bile in nonobese and normolipidemic Japanese patients with cholesterol gallstones does not result from an increased hepatic cholesterol synthesis or a decreased bile acid synthesis.

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Primary dual defect of cholesterol and bile acid metabolism in liver of patients with intrahepatic calculi

Shoda

Tanaka

et al. 1995

Gastroenterology

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Postnatal developmental profile of 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthetase and cholesterol 7α-hydroxylase activities in the liver of domestic swine

Cited by 5 publications

References 26 publications

Expression of key enzymes in bile acid biosynthesis during development: CYP7B1-mediated activities show tissue-specific differences

Expression of key enzymes in bile acid biosynthesis during development: CYP7B1-mediated activities show tissue-specific differences

Hepatic cholesterol and bile acid synthesis in Japanese patients with cholesterol gallstones

Primary dual defect of cholesterol and bile acid metabolism in liver of patients with intrahepatic calculi

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