Postoperative Plasma Metabolic Consequences of an Osseous Substitute Implantation: Analysis by Fourier Transform Infrared Spectroscopy

Abstract

In this study, we have investigated the postoperative plasma consequences of coral implantation into femoral condyle of rabbits. Analyses were performed using Fourier transform infrared (FT-IR) spectroscopy, a sensitive and nondestructive method, to evaluate plasma modifications one day, one week, one month, and three months after surgery. This technique showed that both surgery and implantation induced important changes of lipidic compounds one day after surgery, with a body lipolysis. Major modifications appeared one week after surgery, with a decrease of protein and saccharide contents, and an increase of amino acids accompanied by a noticeable inflammatory process. These last changes were revealed by a hierarchical classification in the 1430-1295 and 1137-1092 cm(-1) spectral regions, leading to two well-separated groups, before and one week after surgery. After a delayed time of three months and despite a substantial recovery for biomolecules such as proteins and fatty acids, some alterations are always observed, such as for amino acids, triglycerides, and glycerol. Moreover, molecules implicated in the inflammatory process had not reached their initial level.

Mechanical improvement of calcium carbonate cements by in situ HEMA polymerization during hardening

“…Hetero-or autografts or implants of natural origin (e.g. calcareous corals) are often used, 1,2 however, they come with major drawbacks, such as biological variability and risk of viral or bacterial contaminations. In a warming world, we even may have to face an increasing uncertainty in supply when it comes to corals.…”

Preparation, physical–chemical characterisation and cytocompatibility of calcium carbonate cements

“…Many surgeons use bone hetero-or autografts or implants of natural origin (coral for example) [1][2][3][4][5][6]. However, supply difficulty, biological variability and viral or bacterial contamination risks are major drawbacks [6][7].…”