Postpartum umbilical cord blood collection for transplantation: A comparison of three methods

Elchalal, Uriel; Fasouliotis, Sozos J.; Shtockheim, David; Brautbar, Chaim; Schenker, Joseph G.; Weinstein, D; Nagler, Arnon

doi:10.1016/s0002-9378(00)70517-5

Cited by 45 publications

(40 citation statements)

References 22 publications

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“…Also, the potential risk of maternal cell contamination could be increased. 5 Based on our findings, we conclude that the mode of collection influences the haematopoietic content of CB donations. Collection before placental delivery is the best approach for CB collection to allow optimisations of CB bank methodology.…”

Section: Discussionmentioning

confidence: 54%

“…Open systems have mostly been discarded because they result in excessive microbiological contamination, as shown by some authors. 5 Our results show microbiological contaminations were higher for ex utero collections. This could be explained by manipulation of placenta after delivery as compared to in utero collections.…”

Section: Discussionmentioning

confidence: 56%

“…4 On the other hand, open systems, which were initially used for CB collection, have been replaced by closed systems with blood collection bags to minimise the risk of microbiological contamination. 5 In this study, the benefits and disadvantages between two different CB collection strategies were evaluated, in order to improve CB bank methodology.…”

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confidence: 99%

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Comparison between two strategies for umbilical cord blood collection

Solves¹,

Moraga

Saucedo

et al. 2003

Bone Marrow Transplant

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Summary:The use of cord blood (CB) for transplantation has increased greatly in recent years. The collection strategy is the first step in collecting good-quality CB units. There are two main techniques for collecting CB from the umbilical vein: in the delivery room while the placenta is still in the uterus by midwives and obstetricians or in an adjacent room after placental delivery by CB bank trained personnel. In this study, the benefits and disadvantages between the two different CB collection strategies were evaluated, in order to improve CB bank methodology. Valencia CB bank maintains the two different collection strategies. CB was obtained from 569 vaginal and 70 caesarean deliveries and obstetrical and clinical charts were reviewed. Before processing CB units, volume was calculated and samples were drawn for cell counts. After processing and before cryopreservation samples were drawn for cell counts, CD34+cell analysis, viability, clonogenic assays and microbiology were drawn directly from the bags. We compared the efficiency of the two collection techniques. Obstetric data and umbilical CB were obtained from 569 vaginal (264 collected in utero and 305 collected ex utero) and 70 caesarean deliveries. The proportion of excluded CB units before processing was 33% for vaginal ex utero, 25% for vaginal in utero and 46% for caesarean deliveries. Differences were statistically significant. For vaginal deliveries a larger volume and a higher number of nucleated cells, percentage of CD34+ cells and colony-forming units (CFUs) were harvested in the in utero collection group. There was no statistical difference between CB collected after placental expulsion from vaginal and caesarean deliveries. Comparison between all vaginal and caesarean deliveries did not show any difference. We conclude that the mode of collection influences the haematopoietic content of CB donations. Collection before placental delivery is the best approach to CB collection and allows optimisation of CB bank methodology. Caesarean deliveries seem to contain similar progenitor content to vaginal deliveries.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 56%

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Comparison between two strategies for umbilical cord blood collection

Solves¹,

Moraga

Saucedo

et al. 2003

Bone Marrow Transplant

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show abstract

“…For example the use of three different methods of UCB collection has been studied in 75 women with uncomplicated vaginal delivery: method 1 -collection of blood into a standard donation bag; methods 2 and 3 -withdrawal of cord blood by a syringe until the delivery of the placenta, followed by flushing one of the umbilical arteries through a catheter with sodium chloride solution and collection of cord blood either into an open sterile container (method 2) or into a standard donation blood bag (method 3). The third one was found the most effective method for UCB collection because it doubles the total white blood cells collected and increases the volume collected with an acceptable risk of bacterial contamination [15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Differences in Umbilical Cord Blood Units Collected during Cesarean Section, before or after the Delivery of the Placenta

Pafumi

Farina²,

Bandiera³

et al. 2002

Gynecol Obstet Invest

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Umbilical cord blood is largely employed as an alternative source of stem cells in the treatment of hemato-oncological diseases. Current results show that the success rate of purified umbilical cord blood engraftment is comparable to that obtained using bone marrow, and it is directly related to the number of pluripotent stem cells transplanted. The technique of fetal blood collection varies among different umbilical cord blood banks. Many authors collect umbilical cord blood during vaginal delivery, after placental detachment, while others collect it while the placenta is still within the uterus. In a previous randomized trial, we showed a greater collection of umbilical cord blood before placental detachment during vaginal delivery. The present study was performed to determine whether umbilical cord blood collection before placental detachment (group A) during cesarean section is superior to that after placental delivery (group B) puncturing the umbilical vein once and using a closed bag system. To accomplish this, 47 pregnant women subjected to cesarean section were enrolled in the study. Twenty-one of them were allocated to group A, while the remaining 26 formed group B. The volume of umbilical cord blood collected from the patients of group A was greater than that collected from patients of group B. The cord blood volume collected was 90.7 ± 6.0 versus 60.9 ± 13.7 ml; the cord blood nucleated cell number was 10.1 ± 1.2 × 10⁸ vs. 7.1 ± 0.8 × 10⁸; and the mean cord blood CD34+ cell number was 20.0 ± 6.0 × 10⁵ vs. 16.4 ± 2.4 × 10⁵, respectively.

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“…The feasibility of obstetrician-based collection has been shown by many cord blood banks [5] . Open systems, which were initially used for cord blood collection, have been replaced by closed systems with blood collection bags to minimize the risk of microbiological contamination [6] .…”

Section: Mode Of Collection Doesmentioning

confidence: 99%

Mode of Collection Does Not Influence Haematopoietic Content of Umbilical Cord Blood Units from Caesarean Deliveries

Solves¹,

Fillol²,

López

et al. 2006

Gynecol Obstet Invest

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Background: Collection strategy is the first step for collecting good quality cord blood units. There are two main different techniques for collecting cord blood from the umbilical vein: in the delivery room while the placenta is still in the utero by midwifes and obstetricians, or in an adjacent room after placental delivery by cord blood bank trained personal. Our aim was to evaluate the benefits and disadvantages between the two different cord blood collection strategies in caesarean deliveries. Methods: We retrospectively analysed data of cord blood units collected from caesarean deliveries for a 3-year period. Caesarean section was performed with a low uterine transversal incision in all patients according to common obstetrical practice. Cord blood collection was performed before or after placental delivery. Results: Obstetrical and umbilical cord blood data was obtained from 253 caesarean deliveries. No statistically significant difference was observed for obstetrical variables or cord blood variables except for Hct and platelets. Conclusions: We conclude both methods produce comparable TNC, CD34 and CFU counts of cord blood units collected from caesarean sections. Before placental delivery collection avoids the financial investment that generates the presence of cord blood banking personal in the maternity ward.

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Postpartum umbilical cord blood collection for transplantation: A comparison of three methods

Cited by 45 publications

References 22 publications

Comparison between two strategies for umbilical cord blood collection

Comparison between two strategies for umbilical cord blood collection

Differences in Umbilical Cord Blood Units Collected during Cesarean Section, before or after the Delivery of the Placenta

Mode of Collection Does Not Influence Haematopoietic Content of Umbilical Cord Blood Units from Caesarean Deliveries

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