Postsynaptic activity of inhibitory neurons evokes hemodynamic fMRI responses

Poplawsky, Alexander John; Iordanova, Bistra; Vazquez, Alberto L.; Kim, Seong‐Gi; Fukuda, Mitsuhiro

doi:10.1016/j.neuroimage.2020.117457

Cited by 12 publications

(10 citation statements)

References 129 publications

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“…However, we did not find depth-dependent variations of BOLD responses likely due to high BOLD responses in draining vessels. To compare depth-dependent neural activity and hemodynamic responses, laminar-specific CBF or CBV measurements ( Poplawsky et al. 2015 ) are necessary.…”

Section: Discussionmentioning

confidence: 99%

“…1998 ; Poplawsky et al. 2015 ; Poplawsky et al. 2021 ) or by selectively activating inhibitory neurons ( Anenberg et al.…”

Section: Introductionmentioning

confidence: 99%

“…1998 ) and olfactory bulb ( Poplawsky et al. 2015 ; Poplawsky et al. 2021 ), indicating that inhibitory neuronal activity induces a positive BOLD response.…”

Section: Introductionmentioning

confidence: 99%

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Contribution of Excitatory and Inhibitory Neuronal Activity to BOLD fMRI

Moon

Jiang

et al. 2021

Cerebral Cortex

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The BOLD fMRI response in the cortex is often assumed to reflect changes in excitatory neural activity. However, the contribution of inhibitory neurons to BOLD fMRI is unclear. Here, the role of inhibitory and excitatory activity was examined using multimodal approaches: electrophysiological recording, 15.2 T fMRI, optical intrinsic signal imaging, and modeling. Inhibitory and excitatory neuronal activity in the somatosensory cortex were selectively modulated by 20-s optogenetic stimulation of VGAT-ChR2 and CaMKII-ChR2 mice, respectively. Somatosensory stimulation and optogenetic stimulation of excitatory neurons induced positive BOLD responses in the somatosensory network, whereas stimulation of inhibitory neurons produced biphasic responses at the stimulation site, initial positive and later negative BOLD signals, and negative BOLD responses at downstream sites. When the stimulation duration was reduced to 5 s, the hemodynamic response of VGAT-ChR2 mice to optogenetic stimulation was only positive. Lastly, modeling performed from neuronal and hemodynamic data shows that the hemodynamic response function (HRF) of excitatory neurons is similar across different conditions, whereas the HRF of inhibitory neurons is highly sensitive to stimulation frequency and peaks earlier than that of excitatory neurons. Our study provides insights into the neurovascular coupling of excitatory and inhibitory neurons and the interpretation of BOLD fMRI signals.

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Section: Discussionmentioning

confidence: 99%

“…1998 ; Poplawsky et al. 2015 ; Poplawsky et al. 2021 ) or by selectively activating inhibitory neurons ( Anenberg et al.…”

Section: Introductionmentioning

confidence: 99%

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Contribution of Excitatory and Inhibitory Neuronal Activity to BOLD fMRI

Moon

Jiang

et al. 2021

Cerebral Cortex

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“…The relationship between BOLD signals and the release of GABA is also an important topic, which was previously investigated using optical intrinsic signal imaging. 53 While this work did not focus on BOLD signals, our tool will be useful for investigating unsolved questions in relation to brain function.…”

Section: Discussionmentioning

confidence: 99%

MRS-measured glutamate versus GABA reflects excitatory versus inhibitory neural activities in awake mice

Takado

Takuwa

Sampei

et al. 2021

J Cereb Blood Flow Metab

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To assess if magnetic resonance spectroscopy (MRS)-measured Glutamate (Glu) and GABA reflect excitatory and inhibitory neural activities, respectively, we conducted MRS measurements along with two-photon mesoscopic imaging of calcium signals in excitatory and inhibitory neurons of living, unanesthetized mice. For monitoring stimulus-driven activations of a brain region, MRS signals and mesoscopic neural activities were measured during two consecutive sessions of 15-min prolonged sensory stimulations. In the first session, putative excitatory neuronal activities were increased, while inhibitory neuronal activities remained at the baseline level. In the second half, while excitatory neuronal activities remained elevated, inhibitory neuronal activities were significantly enhanced. We assessed regional neurochemical statuses by measuring MRS signals, which were overall in accordance with the neural activities, and neuronal activities and neurochemical statuses in a mouse model of Dravet syndrome under resting condition. Mesoscopic assessments showed that activities of inhibitory neurons in the cortex were diminished relative to wild-type mice in contrast to spared activities of excitatory neurons. Consistent with these observations, the Dravet model exhibited lower concentrations of GABA than wild-type controls. Collectively, the current investigations demonstrate that MRS-measured Glu and GABA can reflect spontaneous and stimulated activities of neurons producing and releasing these neurotransmitters in an awake condition.

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“…NO mediates the brain vessels dilation which follows increased neuronal activity leading to the increased need for O2 supply [41,42]. In this perspective, we may expect that hypnotizability-related differences in the cerebral artery diameters occur during cognitive tasks.…”

Section: Changes In the Cerebral Arteries Diameter Induced By Brain A...mentioning

confidence: 99%

Hypnotizability-Related Cerebral Blood Flow

Rashid¹,

Santarcangelo²,

Roatta³

2022

Preprint

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Hypnotizability is a cognitive trait associated with differences in the brachial artery flow-mediated dilatation of individuals with high hypnotizability (highs) and low hypnotizability scores (lows). The study investigated possible hypnotizability-related cerebrovascular differences. Among 24 healthy volunteers the Stanford Hypnotic Susceptibility Scale Form A, identified 13 medium-to-lows (med-lows), 11 medium-to-highs (med-highs), 1 medium hypnotizable. Hypnotizability did not influence the significant changes produced by the trail making task (TMT), mental arithmetic task (MAT), hyperventilation (HVT) and rebreathing (RBT) on Heart rate (HR), arterial blood pressure (ABP) and partial pressure of end-tidal CO2 (PETCO2) and oxygenation (TOI); but moderated the correlations between the changes occurring during tasks with respect to basal conditions (Δ) in ABP and PETCO2 with MCAv. In HVT, med-lows exhibited a significant correlation between ΔMCAv and ΔPETCO2, med-highs showed a significant correlation between ΔABP and ΔMCAv; a significant correlation between ΔTHI and ΔTOI was observed in medium lows. Cerebrovascular reactivity (CVR) and conductance (ΔCVCi) were significantly correlated with ΔMCAv in med-lows during HVT and RBT. Findings represent the first assessment of hypnotizability-related differences in the mechanisms controlling the middle cerebral artery flow velocity, cerebrovascular reactivity and conductance in response to hyperventilation and rebreathing.

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Postsynaptic activity of inhibitory neurons evokes hemodynamic fMRI responses

Cited by 12 publications

References 129 publications

Contribution of Excitatory and Inhibitory Neuronal Activity to BOLD fMRI

Contribution of Excitatory and Inhibitory Neuronal Activity to BOLD fMRI

MRS-measured glutamate versus GABA reflects excitatory versus inhibitory neural activities in awake mice

Hypnotizability-Related Cerebral Blood Flow

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