Postthaw Addition of Autologous Seminal Plasma Improves Sperm Motion Characteristics in Fair and Poor Freezer Stallions

Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance

“…While in cattle, selection of sires is based on the assessment of reproductive parameters, including sperm freezability, stallions are selected according to a specific phenotype mainly related to sport performance, which is not usually in line with sperm cryotolerance [1,4]. Moreover, in horses, as in other mammalian species, there is a high individual variability in the ability of sperm to withstand freezing and thawing procedures [4,5,6,7,8]. These differences, which appear to be partially related to the lipid composition of sperm plasma membrane, lead stallions to be classified as of “good” or “poor” freezers [4,6].…”

“…Seminal plasma, which is the fluid containing the sperm at ejaculation, is produced by the epididymis and accessory sex glands, and is made up of proteins, ions, and organic substances, such as amino acids, lipids, monosaccharides, and hormones [7,9]. In mammals, this fluid is known to play a vital role for sperm function, both in the male and female reproductive tract [10]; however, conflicting results have been reported with regard to their beneficial or detrimental effects during storage of cooled and cryopreserved equine semen [6,8,9,11]. For this reason, most of the seminal plasma is usually discarded before cryopreservation, as this minimizes the negative impact on sperm motility and viability during sperm storage.…”

“…For this reason, most of the seminal plasma is usually discarded before cryopreservation, as this minimizes the negative impact on sperm motility and viability during sperm storage. However, the inclusion of a low proportion of seminal plasma, from 1% to 20%, has been reported to have a positive effect on sperm motion characteristics [7,8,12].…”

Characteristics, Yield, Flow Cytometer, DNA Fragmentation, and Comet Assay Parameters of Goat Spermatozoa in Semen of Zaraibi and Baladi Bucks at Young and Old Ages

“…Consistent with the results obtained by García-Ferreyra et al (2012), the DNA fragmentation, progressive motility, and morphology of sperm cells are associated with advanced paternal age. The effect of DNA integrity of spermatozoa on the fertility and semen quality has been studied in stallion (Neuhauser et al, 2019), pig (Khezri et al, 2019), and ram (Falchi et al, 2018;Peris-Frau et al, 2019). In this way, Belloc et al (2009) found significant increase in DNA damage of spermatozoa with male age progress.…”

How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing

“…Interestingly, the results of a study by de Andrade et al [13] showed that the addition of autologous SP from good freezing stallions to semen after thawing only increased ALH. On the other hand, Neuhauser et al [25] published increased CASA parameters when autologous SP was added after thawing. In our experiment, the subsequent 30 min incubation of semen with both S-SP and A-SP benefitted the kinematic parameters of spermatozoa.…”