Posttranslational Modifications in Algae: Role in Stress Response and Biopharmaceutical Production

Ahmad, Parvez; Bano, Fareha

doi:10.1016/b978-0-12-811913-6.00012-6

Cited by 4 publications

(3 citation statements)

References 115 publications

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“…Other common PTMs of marine microalgae include phosphorylation, glycosylation and disulde bridge, which have assisted recombinant therapeutic protein production. 148 Marine microalgae have been an essential source of valuable natural products. There have been different efforts to engineer these unicellular organisms into cell factories to increase their production capacity.…”

Section: Reviewmentioning

confidence: 99%

Embracing the era of antimicrobial peptides with marine organisms

Chen,

Ye,

et al. 2024

Nat. Prod. Rep.

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Section: Reviewmentioning

confidence: 99%

Embracing the era of antimicrobial peptides with marine organisms

Chen,

Ye,

et al. 2024

Nat. Prod. Rep.

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“…Oligosaccharides and linkages in the content of N-glycan are represented their symbols and abbreviations ( Figure 1-C) [11,12]. N-glycosylation is the most common PTM of mammalian proteins and has various functional roles that affect the properties of the protein such as folding, stability, solubility, and intracellular trafficking [13][14][15]. Within the immune system, the N-glycans on an immune cell's surface will help dictate that migration pattern of the cell.…”

Section: Introductionmentioning

confidence: 99%

N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis

Yaman

Aladağ

Kayili

et al. 2021

Hacettepe Journal of Biology and Chemistry

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G ünümüzde, mikroalg türlerinin biyofarmasötik üretiminde hammadde olarak kullanımı konusu tartışılmaktadır. .Bu fikrin altında yatan temel neden mikro alglerin, biyolojik olarak aktif çok sayıda bileşenin üretimi için karbon dioksiti etkin bir şekilde kullanabilen hücre fabrikaları olmasıdır. Bununla birlikte, mikroalglerden rekombinant protein üretiminde kullanılması için çözülmesi gereken birkaç sorun mevcuttur. Kritik gereksinimlerden birisi salgılanan rekombinant proteinlerden biyo-uyumlu bir N-glikozilasyon profile üretmektir. Mikroalg türlerinin glikozilasyon makineleri ve N-glikan profilleri hakkındaki bilgiler oldukça sınırlıdır. Bu çalışmada, yeşil bir mikroalg türü olan Chlorella zofingiensis 'in N-glikan profilinin karakterize edilmesi amaçlandı. Bu amaçla (gliko-)proteinleri içeren fotoototrofik olarak yetiştirilmiş Chlorella zofingiensis ekstreleri enzimatik olarak deglikozile edildi ve 2-aminobenzoik asit etiketi ile etiketlendi. Serbest hale getirilen N-glikanlar HILIC bazlı bir yaklaşımla saflaştırıldı ve MALDI-TOF (/TOF)-MS ile analiz edildi. Sonuçlar C. zofingiensis'in oligomannozidik türde N-glikan modellerini içerdiğini göstermiştir. Ek olarak, MALDI-MS tarafından C. zofingiensis'in N-glikosilasyon profilleri, oligomannosidik N-glikanların neredeyse yarısının fosforilenmiş olduğunu ortaya koymuştur.

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“…Microalgae can carry out many of the post-translational mechanisms needed to produce many complex proteins and are relatively easy and cheap to culture. They require fundamental media to grow on, such as minimal or acetate-based media with a few trace elements and the ability to use sunlight as an energy source; hence the cost of goods is low [17].…”

mentioning

confidence: 99%

Development of Cost-Effective Fertilizer-Based Media for the Microalgae Cultivation Aimed at Effective Biomass Production

et al. 2020

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In the diversification of the world's energy portfolio, microalgae biofuels have gained particular attention as a means of providing carbon-neutral hydrocarbon-based fuels. The progress on this front has been hindered because of expensive and unreliable microalgae cultivation systems, where the preparation of synthetic culture media poses a monetary strain. This study aims to investigate a new microalgae growth media that is readily available and cost-effective. The new microalgae fertilizerbased media (FBM) were designed and compared with Bold Basal media (BBM) in terms of growth parameters, production of lipid and value-added algal by-products (carotenoid and phycobiliprotein). The growth parameters such as cell count and specific growth rate were investigated for FBM and BBM. FBM showed a significant growth rate of 7 million/mL-per day as compared to 2 million/ml per day. A similar growth trend was observed for chlorophyll (a), (b) and carotenes which increased to the values of 5microgram/gram formula weight (µg/gfw), 3 µg/gfw and 2 µg/gfw for FBM. On the other hand, lipid and phycobiliprotein showed a decrease of 18.75 % and 16.66 %, respectively, compared to BBM. Even though some growth parameters are negatively affected by fertilizer-based media, the cost reduction is substantial to allow this drawback to be overlooked. The medium's cost is reduced by a factor of 7 and provides ground for the use of the FBM in the large-scale cultivation of algae for biomass production.

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Posttranslational Modifications in Algae: Role in Stress Response and Biopharmaceutical Production

Cited by 4 publications

References 115 publications

Embracing the era of antimicrobial peptides with marine organisms

Embracing the era of antimicrobial peptides with marine organisms

N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis

Development of Cost-Effective Fertilizer-Based Media for the Microalgae Cultivation Aimed at Effective Biomass Production

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