Postweaning low-calcium diet promotes later-life obesity induced by a high-fat diet

He, Yonghan; Li, Songtao; Wang, Yanyan; Wang, Guan; He, Ying; Liao, Xi-Lu; Sun, Changhao; Li, Ying

doi:10.1016/j.jnutbio.2011.07.004

Cited by 16 publications

(11 citation statements)

References 45 publications

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“…Post weaning low calcium diet decreases the AMPK activation in skeletal muscle. 15 On the other hand, HFD leads to obesity which further catalyzes the progression of NAFLD and non-alcoholic steatohepatitis (NASH). 16 HFD induced obesity not only promotes hepatic oxidative stress and triggers various inflammatory processes 17 but also reduces the activation of AMPK which further stimulates the metabolic disturbances during NAFLD.…”

Section: Introductionmentioning

confidence: 99%

Calcium supplementation shows a hepatoprotective effect against high-fat diet by regulating oxidative-induced inflammatory response and lipogenesis activity in male rats

Das

Choudhuri

2020

Journal of Traditional and Complementary Medicine

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Background and aim High-fat diet (HFD) triggers obesity-related metabolic diseases like non-alcoholic fatty liver diseases (NAFLD). Calcium supplementation is known to have an anti-obesity effect. However, the effect of calcium supplementation has not been evaluated so far in context to hepatic functions on exposure to HFD. The goal of the present study was to investigate the role of calcium supplementation on hepatic function and other physiological markers in HFD induced NAFLD rats. Experimental procedure 18 male Wistar rats were divided into two groups; first group considered control group (n = 6) for the entire treatment period and the second group (n = 12) fed with HFD for 6 weeks to induce NAFLD model and then sub-divided into two groups (n = 6 rats); one group received HFD and other group received 1.0 gm CaCO 3 /100 gm HFD for 30 days. After treatment, all animals were euthanized to collect the blood and liver for biochemical, enzymatic, oxidative, anti-oxidant, western blot and histological study. Results and conclusion Calcium supplementation significantly improved the anthropometric parameters and decreases the level of serum cholesterol, triglyceride, FFA and hepatic enzymes. Calcium supplementation significantly down-regulated the hepatic PPAR-γ mediated FAS activity, hepatic lipid accumulation, oxidative stress and restored the activities of antioxidant enzyme which further prevented the stimulation of pro-inflammatory response. Calcium supplementation also increases the hepatic protein expression of phosphorylated AMP-activated protein kinase. So, calcium supplementation showed a hepatoprotective effect during NAFLD by downregulating the oxidative induced inflammatory response stimulated by hepatic lipogenesis activity and subsequent lipid accumulation.

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Section: Introductionmentioning

confidence: 99%

Calcium supplementation shows a hepatoprotective effect against high-fat diet by regulating oxidative-induced inflammatory response and lipogenesis activity in male rats

Das

Choudhuri

2020

Journal of Traditional and Complementary Medicine

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“… 13 , 14 Dietary calcium or VitD 3 (cholecalciferol) intake, as well as calcitriol administration have been shown to prevent adiposity, insulin resistance 14 , 15 and hepatic fatty changes 16 , 17 and to be related to AMPK activation in different tissues in animal models. 18 , 19 On the other hand, Shalata et al . 20 reported that vitamin D could intensify the effects of sitaglibtin/metformin drugs on treatment of steatosis and on decreasing hepatic triglyceride and malondialdehyde (MDA) content.…”

Section: Introductionmentioning

confidence: 99%

The Eeffect of Metformin Combined with Calcium-Vitamin D3 Against Diet-Induced Nonalcoholic Fatty Liver Disease

et al. 2018

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Purpose: Metformin is one of the most popular drugs tested against nonalcoholic fatty liver disease (NAFLD). The present study aimed to investigate whether calcium-vitamin D3 cosupplementation will intensify the effect of metformin on the prevention of high-fat, high-fructose (HFFr) diet-induced hepatic steatosis.Methods: Male wistar rats (210±16 g) were assigned into the following seven groups: a Control group to receive a standard chow and six HFFr-fed groups to receive diets containing either normal (0.5% calcium and 1000 IU/kg vitamin D3) or high amount of calcium and vitamin D3 (2.4% calcium and 10000 IU/kg vitamin D3) (CaD), in combination with gastric gavage administration of either saline or 25 or 200 mg/kg body weight/day metformin. After 60 days, rats were assessed with respect to their anthropometric, metabolic and hepatic parameters, as well as their hepatic AMP-activated protein kinase (AMPK) phosphorylation.Results: Metformin and CaD, either alone or in combination, caused a significant reduction in HFFr diet-induced high serum aspartate aminotransferase (AST), hepatic steatosis and lipid accumulation without effect on insulin resistance and AMPK phosphorylation. In addition, slightly (and non-significantly) better effects of the combination in ameliorating steatosis and hepatic cholesterol content were observed.Conclusion: Taken together, our results suggest that metformin and CaD could protect against the onset of HFFr diet-induced NAFLD in an insulin and AMPK-independent manner, without any marked additional benefits of their combination.

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“…Calcium, in which O. humifusa is rich, plays an important role in the modulation of the intracellular signaling second messenger that leads to muscle contraction and gene expression. Increased calcium intake is highly involved in improving lipid metabolism, oxidative phosphorylation, mitochondrial biogenesis, and PGC-1α protein expression due to an increase in the activation of AMP-activated protein kinase (AMPK) and calcium/calmodulin-dependent protein kinase (CAMK) [15,23,34]. As a result, we suggest that the intake of calcium-rich O. humifusa increases the activation of intramuscular AMPK and CAMK by increasing the serum calcium level, resulting in the expression of PPAR-γ and PGC-1 α, which are involved in lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Opuntia humifusa Supplementation Reduces Fat Weight by Increasing PPAR-γ and PGC-1α Protein Expression in the Skeletal Muscle of Rats

Kwon¹,

Kang²,

Kim³

et al. 2014

Journal of Life Science

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This study was conducted to investigate the effects of supplementation with Opuntia humifusa on the expression of peroxisome proliferator-activated receptor-delta (PPAR-δ), peroxisome proliferator-activated receptor-gamma (PPAR-γ) and peroxisome proliferator-activated receptor gamma coactivator1alpha (PGC-1α) in the skeletal muscle of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats at 6 weeks of age were randomly divided into 2 groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). The rats were fed a high-fat diet (CG) or a high-fat diet supplemented with 5% O. humifusa (EG) for 8 weeks. The results showed that the abdominal fat pad and epididymal fat pad weights were significantly lower in the EG than in the CG (p<0.01). In the blood, serum glucose, triglycerides, and total cholesterol in the EG group were lower than in the CG (p<0.01). The expression of PPAR-γ and PGC-1α protein in the skeletal muscle of the EG was increased compared with that of the CG (p<0.05). These results indicate that 8 weeks of O. humifusa supplementation lowers serum glucose and triglyceride levels and suppresses weight gain by reducing fat weight through an increase in the expression of PPAR-γ and PGC-1α in the muscle tissue of rats.

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Postweaning low-calcium diet promotes later-life obesity induced by a high-fat diet

Cited by 16 publications

References 45 publications

Calcium supplementation shows a hepatoprotective effect against high-fat diet by regulating oxidative-induced inflammatory response and lipogenesis activity in male rats

Calcium supplementation shows a hepatoprotective effect against high-fat diet by regulating oxidative-induced inflammatory response and lipogenesis activity in male rats

The Eeffect of Metformin Combined with Calcium-Vitamin D3 Against Diet-Induced Nonalcoholic Fatty Liver Disease

Opuntia humifusa Supplementation Reduces Fat Weight by Increasing PPAR-γ and PGC-1α Protein Expression in the Skeletal Muscle of Rats

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