Potato

Bradeen, James M.; Carputo, Domenico; Douches, David S.

doi:10.1002/9781405181099.k0704

Cited by 4 publications

(2 citation statements)

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“…They should be used in breeding strategies based upon somatic hybridization or bridge crosses (Jansky, 2006). Of course, all resistant genotypes can be employed to clone resistant genes for transgenic approaches, as has already been done in S. bulbocastanum for late blight resistance and S. spegazzinii for nematode resistance (reviewed in Bradeen et al (2008)). Interestingly, we found two S. commersonii genotypes (Cmm1T and Cmm6-6) contrasting for resistance traits (e.g.…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers

Carputo

Alioto

Garramone

et al. 2013

Plant Genet. Resour.

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The evolutionary diversity of wild potato species makes them excellent materials for improving the narrow genetic basis of the cultivated potato Solanum tuberosum. Understanding their genetic diversity is important not only to choose the best parents for breeding, but also to design proper crossing schemes and selection strategies. The objectives of this study were to determine the resistance response to Ralstonia solanacearum, Potato virus Y and low temperatures of 21 clones of 12 potato species, and to determine their genetic diversity through simple sequence repeat (SSR) markers. Sources of resistance have been found for all the investigated traits, with high resistance variability not only between but also within species. Combined resistances were also identified, with positive implications for efficient breeding. SSR analysis allowed the detection of 12 loci and 46 alleles across all genotypes, with an average value of 3.8 alleles per locus. Both unique and rare alleles useful for marker-assisted selection were found. SSR-based cluster analysis revealed that resistant genotypes were distributed among all clusters, suggesting that genetically different resistant genotypes were identified. The information obtained in this study is discussed from a breeding perspective

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Section: Discussionmentioning

confidence: 99%

“…Alternatively, strategies based on either somatic hybridization or bridge crosses can be employed (Jansky, 2006). Genotypes carrying genes of agricultural interest to potato can also be used to clone relevant genes for a direct transfer to the cultivated potato through genetic engineering (Bradeen et al , 2008).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers

Carputo

Alioto

Garramone

et al. 2013

Plant Genet. Resour.

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Disease resistance gene transcription in transgenic potato is unaltered by temperature extremes and plant physiological age

et al. 2011

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To date, several dozen plant disease resistance genes have been cloned from a variety of species. Despite successful efforts to isolate functional disease resistance genes and understand their roles in defence responses, surprisingly little is known about their transcriptional regulation. Global climate changes are expected to impact crop plant production, plant physiology and plant-microbe interactions. Understanding the impact of varying environmental and physiological factors on disease resistance gene transcript levels may be key to successful deployment. In this study we analyzed the expression patterns of the Phytophthora infestans resistance gene RB in transgenic potato plants across a broad range of temperatures and key physiological stages. Our results demonstrate that while temperature extremes have an obvious effect on plant morphology and development, RB is transcribed at all ages and temperatures in all genetic backgrounds. Quantitative analyses demonstrate that the RB transcript accumulates comparably throughout plant development, including at the tuber initiation stage. Implications for successful deployment of RB are discussed.

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Higher Copy Numbers of the Potato RB Transgene Correspond to Enhanced Transcript and Late Blight Resistance Levels

Bradeen¹,

Iorizzo²,

Mollov³

et al. 2009

MPMI

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Late blight of potato ranks among the costliest of crop diseases worldwide. Host resistance offers the best means for controlling late blight, but previously deployed single resistance genes have been short-lived in their effectiveness. The foliar blight resistance gene RB, previously cloned from the wild potato Solanum bulbocastanum, has proven effective in greenhouse tests of transgenic cultivated potato. In this study, we examined the effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions. In a two-year replicated trial, the RB transgene, under the control of its endogenous promoter, provided effective disease resistance in various genetic backgrounds, including commercially prominent potato cultivars, without fungicides. RB copy numbers and transcript levels were estimated with transgene-specific assays. Disease resistance was enhanced as copy numbers and transcript levels increased. The RB gene, like many other disease resistance genes, is constitutively transcribed at low levels. Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested. We conclude that even in these ultra-high copy number lines, innate RNA silencing mechanisms have not been fully activated. Our findings suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing. Strategies for the deployment of RB are discussed in light of the current research.

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Potato

Cited by 4 publications

References 202 publications

Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers

Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers

Disease resistance gene transcription in transgenic potato is unaltered by temperature extremes and plant physiological age

Higher Copy Numbers of the Potato RB Transgene Correspond to Enhanced Transcript and Late Blight Resistance Levels

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