Potato dehydrins present high intrinsic disorder and are differentially expressed under ABA and abiotic stresses

Charfeddine, Safa; Charfeddine, Mariam; Saïdi, Mohammed Najib; Jbir, Rania; Bouzid, Radhia Gargouri

doi:10.1007/s11240-016-1120-4

Cited by 13 publications

(4 citation statements)

References 63 publications

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“…These genes exhibit key differences in the promoter regions, suggesting differences in the stress triggers and response to different stresses. One specific LEA gene was expressed in all tissues of the plant when exposed to salinity and only in the roots when exposed to drought; its expression was also repressed by exogenous ABA application (Charfeddine et al 2015(Charfeddine et al , 2017a. Whether expression levels of this gene can be biotechnologically modified to improve salt tolerance has yet to be tested.…”

Section: Impacts Of Greater Salinity On Potatomentioning

confidence: 99%

Climate Change and Consequences for Potato Production: a Review of Tolerance to Emerging Abiotic Stress

et al. 2017

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Potato is a major global crop that has an important role to play in food security, reducing poverty and improving human nutrition. Enhanced atmospheric CO 2 concentrations provide an opportunity to increase potato yields in the future, but this will only be possible if the potato crop can cope with the other consequences of climate change caused by this rise in CO 2 . While climate change may impact biotic stress either positively or negatively, abiotic stresses are likely to be greatly increased and become a major threat to potato production. Increasing heat, drought and salinity stress will drive the need for greater understanding of genes, traits and management techniques that allow potato to cope with these stresses. In this review we identify some of the key physiological and molecular adaptations of potato to these stresses and propose an ideotype which should include 1) optimal stomatal regulation to balance water loss and heat stress in leaves, 2) production of metabolites and transporters to scavenge reactive oxygen species and partition toxic elements, 3) enhanced root systems to maximise water capture, 4) maintenance of tuberisation under stress conditions and 5) stress avoidance by accelerating crop development and reducing time to yield. We discuss potential ways to achieve this ideotype, emphasising the need to benefit from genetic diversity in landrace and wild material by screening for traits in combined stress environments appropriate to future agroecosystems.

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Section: Impacts Of Greater Salinity On Potatomentioning

confidence: 99%

Climate Change and Consequences for Potato Production: a Review of Tolerance to Emerging Abiotic Stress

et al. 2017

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“…Based on the numbers of Y-, S- and K-segments, DHNs can be subdivided into five major types, including Y n SK n , K n , SK n , Y n K n , and K n S (Close 1996 ; Lv et al 2017 ). Since DHN genes are expressed in various plant tissues including roots, stems, leaves, flowers, fruits and seeds, different types of DHN genes may be involved in response to diverse abiotic stresses including heat and cold (Abedini et al 2017 ; Charfeddine et al 2017 ; Jing et al 2016 ; Verma et al 2017 ). For example, the expression of three DHN genes was detected in sugarcane under heat stress, and their expression is independent of the changes in the water relations of leaves (Wahid and Close 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Zhou

et al. 2017

AMB Expr

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As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11 showed that it is a Y3SK2-type DHN protein rich in hydrophilic amino acids. Expression analyses revealed that the transcription of CsLEA11 could be significantly induced by heat and cold stress. The recombinant plasmid was transformed into Escherichia coli BL21 and isopropy-β-d-thiogalactoside (IPTG) was used to induce recombinant E. coli to express CsLEA11 gene. Overexpression of CsLEA11 in E. coli enhanced cell viability and conferred tolerance to heat and cold stress. Furthermore, CsLEA11 protein could protect the activity of lactate dehydrogenase (LDH) under heat stress. Taken together, our data demonstrate that CsLEA11 might function in tolerance of cucumber to heat and cold stress.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0483-1) contains supplementary material, which is available to authorized users.

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“…To predict the probable functions of the newly isolated ScBx6 L318 and ScBx7 L318 genes, we have performed the promoter in silico analysis of their upstream regulatory sequences. Currently, the analysis of 5′ cis regulatory sequences is often used to gain information regarding the function of the investigated genes (e.g., Guo et al 2017;Wang et al 2017;Nawkar et al 2017;Charfeddine et al 2017). By applying such analysis, Guo et al (2017) proved that the protein encoded by the BplMYB46 gene, which binds to the MYBCORE and AC-box motifs, plays an important role in secondary cell wall synthesis and response to abiotic stress in Betula platyphylla.…”

Section: Discussionmentioning

confidence: 99%

Isolation and structural analysis of the Bx6 and Bx7 genes controlling the biosynthesis of benzoxazinoids in rye (Secale cereale L.)

Bakera

Rakoczy-Trojanowska

2020

Acta Physiol Plant

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Benzoxazinoids (BXs) are plant secondary metabolites, first discovered in the 1950s, which are synthesized in many monocotyledonous species from the Poaceae family and in several dicotyledonous plants. They constitute a significant element of the plant's defence mechanism against both biotic (pests and diseases) and abiotic (elevated salinity, heavy metals) stresses. The aim of this research was to gain more information on the genetic background of BXs biosynthesis in rye (Secale cereale L.) by isolating and sequencing the ScBx6 L318 and ScBx7 L318 genes encoding 2-oxoglutarate-dependent dioxygenase and 7-O-methyltransferase, respectively. Using the modified Amplicon Express method, BAC clones containing the ScBx6 L318 and ScBx7 L318 genes were isolated and sequenced. The similarity between the ScBx6 L318 and ZmBx6 genes were, correspondingly, 78% and 68% in the coding sequence (cds) and the amino acid sequence (AA). A lesser similarity was found between the ScBx7 L318 and ZmBx7 genes (72% and 45% at the cds and AA levels, respectively).

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Potato dehydrins present high intrinsic disorder and are differentially expressed under ABA and abiotic stresses

Cited by 13 publications

References 63 publications

Climate Change and Consequences for Potato Production: a Review of Tolerance to Emerging Abiotic Stress

Climate Change and Consequences for Potato Production: a Review of Tolerance to Emerging Abiotic Stress

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Isolation and structural analysis of the Bx6 and Bx7 genes controlling the biosynthesis of benzoxazinoids in rye (Secale cereale L.)

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