Potato Suberin Induces Differentiation and Secondary Metabolism in the Genus &lt;i&gt;Streptomyces&lt;/i&gt;

Lerat, Sylvain; Forest, Martin; Lauzier, Annie; Grondin, Gilles; Lacelle, Serge; Beaulieu, Carole

doi:10.1264/jsme2.me11282

Cited by 13 publications

(14 citation statements)

References 36 publications

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“…Supplementation of the growth media with suberoylanilide hydroxamic acid induced several secondary metabolite synthases in A. niger [75] and potato suberin augmented the diversity of the secondary metabolites biosynthesised by Streptomyces sp. [76]. …”

Section: Resultsmentioning

confidence: 99%

Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

et al. 2014

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BackgroundLipid polymers in plant cell walls, such as cutin and suberin, build recalcitrant hydrophobic protective barriers. Their degradation is of foremost importance for both plant pathogenic and saprophytic fungi. Regardless of numerous reports on fungal degradation of emulsified fatty acids or cutin, and on fungi–plant interactions, the pathways involved in the degradation and utilisation of suberin remain largely overlooked. As a structural component of the plant cell wall, suberin isolation, in general, uses harsh depolymerisation methods that destroy its macromolecular structure. We recently overcame this limitation isolating suberin macromolecules in a near-native state.ResultsSuberin macromolecules were used here to analyse the pathways involved in suberin degradation and utilisation by Aspergillus nidulans. Whole-genome profiling data revealed the complex degrading enzymatic machinery used by this saprophytic fungus. Initial suberin modification involved ester hydrolysis and ω-hydroxy fatty acid oxidation that released long chain fatty acids. These fatty acids were processed through peroxisomal β-oxidation, leading to up-regulation of genes encoding the major enzymes of these pathways (e.g. faaB and aoxA). The obtained transcriptome data was further complemented by secretome, microscopic and spectroscopic analyses.ConclusionsData support that during fungal growth on suberin, cutinase 1 and some lipases (e.g. AN8046) acted as the major suberin degrading enzymes (regulated by FarA and possibly by some unknown regulatory elements). Suberin also induced the onset of sexual development and the boost of secondary metabolism.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-613) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

et al. 2014

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“…The stimulating effects of suberin on cellulase-encoding gene transcription may also have resulted from its ability to promote differentiation and secondary metabolism in Streptomyces species ( 32 ). Cellulase activity is low during primary metabolism in some Streptomyces species, but reaches a maximal level during secondary metabolism ( 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the effects of suberin on txt gene expression remain unclear. Lerat et al ( 31 , 32 ) proposed that suberin activated the differentiation and production of secondary metabolites including thaxtomins in several Streptomyces species.…”

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confidence: 99%

Suberin Regulates the Production of Cellulolytic Enzymes in <i>Streptomyces scabiei</i>, the Causal Agent of Potato Common Scab

Padilla-Reynaud

Simao-Beaunoir

Lerat

et al. 2015

Microbes and environments

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Suberin, a major constituent of the potato periderm, is known to promote the production of thaxtomins, the key virulence factors of the common scab-causing agent Streptomyces scabiei. In the present study, we speculated that suberin affected the production of glycosyl hydrolases, such as cellulases, by S. scabiei, and demonstrated that suberin promoted glycosyl hydrolase activity when added to cellulose-, xylan-, or lichenin-containing media. Furthermore, secretome analyses revealed that the addition of suberin to a cellulose-containing medium increased the production of glycosyl hydrolases. For example, the production of 13 out of the 14 cellulases produced by S. scabiei in cellulose-containing medium was stimulated by the presence of suberin. In most cases, the transcription of the corresponding cellulase-encoding genes was also markedly increased when the bacterium was grown in the presence of suberin and cellulose. The level of a subtilase-like protease inhibitor was markedly decreased by the presence of suberin. We proposed a model for the onset of S. scabiei virulence mechanisms by both cellulose and suberin, the main degradation product of cellulose that acts as an inducer of thaxtomin biosynthetic genes, and suberin promoting the biosynthesis of secondary metabolites including thaxtomins.

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“…Geldanamycin was extracted from supernatants of 10-d-old YME cultures ( 63 ) and the antibiotic was quantified by high-performance liquid chromatography using a previously developed method ( 33 ).…”

Section: Methodsmentioning

confidence: 99%

Morphological, Physiological, and Taxonomic Characterization of Actinobacterial Isolates Living as Endophytes of Cacao Pods and Cacao Seeds

Tchinda

Boudjeko

Simao-Beaunoir

et al. 2016

Microbes and environments

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Vascular plants are commonly colonized by endophytic actinobacteria. However, very little is known about the relationship between these microorganisms and cacao fruits. In order to determine the physiological and taxonomic relationships between the members of this community, actinobacteria were isolated from cacao fruits and seeds. Among the 49 isolates recovered, 11 morphologically distinct isolates were selected for further characterization. Sequencing of the 16S rRNA gene allowed the partition of the selected isolates into three phylogenetic clades. Most of the selected endophytic isolates belonged to the Streptomyces violaceusniger clade. Physiological characterization was carried out and a similarity index was used to cluster the isolates. However, clustering based on physiological properties did not match phylogenetic lineages. Isolates were also characterized for traits commonly associated with plant growth-promoting bacteria, including antibiosis and auxin biosynthesis. All isolates exhibited resistance to geldanamycin, whereas only two isolates were shown to produce this antibiotic. Endophytes were inoculated on radish seedlings and most isolates were found to possess plant growth-promoting abilities. These endophytic actinobacteria inhibited the growth of various plant pathogenic fungi and/or bacteria. The present study showed that S. violaceusniger clade members represent a significant part of the actinobacterial community living as endophytes in cacao fruits and seeds. While several members of this clade are known to be geldanamycin producers and efficient biocontrol agents of plant diseases, we herein established the endophytic lifestyle of some of these microorganisms, demonstrating their potential as plant health agents.

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Potato Suberin Induces Differentiation and Secondary Metabolism in the Genus <i>Streptomyces</i>

Cited by 13 publications

References 36 publications

Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

Suberin Regulates the Production of Cellulolytic Enzymes in <i>Streptomyces scabiei</i>, the Causal Agent of Potato Common Scab

Morphological, Physiological, and Taxonomic Characterization of Actinobacterial Isolates Living as Endophytes of Cacao Pods and Cacao Seeds

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