Potato virus Y (PVY) strains in Belgian seed potatoes and first molecular detection of the N-Wi strain

Kamangar, Saman Bahrami; Smagghe, Guy; Maes, Martine; Jonghe, Kris De

doi:10.1007/bf03356485

Cited by 26 publications

(10 citation statements)

References 33 publications

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“…Although the members of PVY N:O/N-Wi share the properties of both parental strains, they tend to induce barely detectable disease that due to mild symptoms remains unnoticed during visual inspection (Gray et al, 2010;Funke et al, 2016;Glais et al, 2017). Furthermore, these strains can also remain latent (symptomless) in potato plants (Glais et al, 2005;Kamangar et al, 2014), which is in line with results of our studies. S. tuberosum L. plantlets cvs.…”

Section: Discussionsupporting

confidence: 90%

Arbuscular Mycorrhiza Changes the Impact of Potato Virus Y on Growth and Stress Tolerance of Solanum tuberosum L. in vitro

et al. 2020

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Under the field conditions crop plants interact with diverse microorganisms. These include beneficial (symbiotic) and phytopathogenic microorganisms, which jointly affect growth and productivity of the plants. In last decades, production of potato (Solanum tuberosum L.) suffers from increased incidence of potato virus Y (PVY), which is one of most important potato pests. Arbuscular mycorrhizal fungi (AMF) are common symbionts of potato, however the impact of mycorrhizal symbiosis on the progression of PVY-induced disease is scarcely known. Therefore, in the present study we investigated the effect of joint PVY infection and mycorrhizal colonization by Rhizophagus irregularis on growth traits of the host potato plant (cv. Pirol). The tested PVY isolate belonged to N-Wilga strain group, which is considered to be predominant in Europe and many other parts of the world. The viral particles were concentrated in the leaves, but decreased the root growth. Furthermore, the infection with PVY evoked prolonged oxidative stress reflected by increased level of endogenous H 2 O 2. AMF alleviated oxidative stress in PVY-infected host plants by a substantial decrease in the level of shoot-and rootderived H 2 O 2 , but still caused asymptomatic growth depression. It was assumed that mycorrhizal symbiosis of potato might mask infection by PVY in field observations.

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Section: Discussionsupporting

confidence: 90%

Arbuscular Mycorrhiza Changes the Impact of Potato Virus Y on Growth and Stress Tolerance of Solanum tuberosum L. in vitro

et al. 2020

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“…At present, the recombinant strains PVY NTN and PVY N W/ N:O are believed to possess a clear fitness advantage over nonrecombinant strains worldwide (Kamangar et al 2014, Karasev et al 2014. Results of monitoring in 2009 to 2011 based on Bavarian seed potato certification (Table 3) showed that ca.…”

Section: Discussionmentioning

confidence: 99%

“…60% were PVY N W. The proportions of these two recombinants in tested PVY infections differ considerably worldwide (Rigotti et al 2011, Yin et al 2012, Kamangar et al 2014. One reason seems to be differences in potato cultivar susceptibility to certain PVY strains, and therefore the ratio between PVY NTN and PVY N W/ N:O probably depends on which potato cultivars are favoured in different countries.…”

Section: Discussionmentioning

confidence: 99%

Potato virus Y (PVY) in Seed Potato Certification

et al. 2015

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In the EU and many other countries worldwide, seed potatoes require certification to be marketed. In most of the European national and international seed potato regulations, the tolerances for viruses are at present based on a combination of virus incidence and symptom severity. Historically all severe viruses were usually the origin of severe virus diseases, and mild viruses, in general, were seen to cause mild diseases. However, the situation has changed, especially for the most economically important virus, Potato virus Y (PVY), which is considered a severe virus. Investigation of PVY-infected potato leaf samples from Bavarian potato seed certification found that the "classical" O and the N strains, predominantly responsible for severe virus diseases, represented less than 1% of the infections present. Ca. 99% of the PVY infections were comprised of the new recombinant strains PVY NTN and PVY N W. The ratio between these recombinants differed substantially. Differences in susceptibility of particular cultivars to certain PVY strains seem to be one reason for that. Therefore, the ratio of the recombinants may depend upon which cultivars are favoured in different potato growing areas or countries.In greenhouse tests, PVY NTN and PVY N W were associated with both severe and mild disease symptoms. In corresponding field tests, the symptom severity differed considerably. Furthermore, there was no correlation detected between virus concentration and symptom severity.These results will contribute to a worldwide discussion on the elimination of the criterion virus symptom severity from potato seed certification during the forthcoming months. The discussion follows amendments made to international seed potato regulation documents. National regulations must be aligned by the EU member states until January 2016.

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“…Similarly, in Tunisia a 67% of PVY NTN variants were reported by Tayahi et al, (2016). Conversely; Kamangar et al, (2014) study from Belgium indicated that strains belonging to the N group were reported to be the most prevalent. Molecular characterization of some Egyptian isolates attempted through sequencing of CP gene followed by phylogenetic analysis, shed light on possible sources of PVY infection in a certain location or governorate.…”

Section: Discussionmentioning

confidence: 96%

The relationship and relativity between three isolates of Potato virus Y Potyvirus infecting potato (Solanum tuberosum L.) at Alexandria and El-Beheira governorates, northern Egypt

El-Aziz

Behiry

Younes

et al. 2019

Novel Research in Microbiology Journal

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Potato virus Y (PVY) is a highly pathogenic virus, causing enormous economic losses in potato (S. tuberosum) crop. Three isolates of PVY were obtained from naturally infected potato plants showing mosaic; yellowing and vein necrosis symptoms, during 2017-2018 growing seasons at certain locations of El-Beheira and Alexandria governorates, Egypt. PVY could be easily transmitted mechanically by aphids. Detection of the PVY-3 in different organs of infected Nicotiana glutinosa plants by Indirect-ELISA; Dot blot immunoassay (DBIA) and Tissue blot immunoassay (TBIA), indicated the possibility of using these methods for viral detection. Egyptian PVY (MK376452) isolate was of close homology to PVY isolated from South Africa. The other Egyptian isolates were found to be close to a French PVY (KJ741115) isolate. There were variations on comparing nucleotide and amino acid sequences; however, nucleotide sequencing could be more reliable. Application of sequence inspection allowed us to identify the PVY isolates by phylogenetic analysis. Phylogenetic analysis of the genomic coat protein (CP) from 24 PVY isolates registered in GenBank indicated the presence of relationships between each other's. This reflected the high degree of genetic variability among our local Egyptian isolates. The aims of the current work were to; isolate and detect PVY from naturally infected potato plants in northern Egypt, characterize the PVY isolates using different assays, detect PVY in different organs of infected potato plants, study the CP gene of the PVY isolates using Reverse transcription-Polymerase Chain Reaction (RT-PCR), and register these isolates in GenBank.

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Potato virus Y (PVY) strains in Belgian seed potatoes and first molecular detection of the N-Wi strain

Cited by 26 publications

References 33 publications

Arbuscular Mycorrhiza Changes the Impact of Potato Virus Y on Growth and Stress Tolerance of Solanum tuberosum L. in vitro

Arbuscular Mycorrhiza Changes the Impact of Potato Virus Y on Growth and Stress Tolerance of Solanum tuberosum L. in vitro

Potato virus Y (PVY) in Seed Potato Certification

The relationship and relativity between three isolates of Potato virus Y Potyvirus infecting potato (Solanum tuberosum L.) at Alexandria and El-Beheira governorates, northern Egypt

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