Potency of an inactivated influenza vaccine prepared from a non-pathogenic H5N1 virus against a challenge with antigenically drifted highly pathogenic avian influenza viruses in chickens

Shichinohe, Shintaro; Okamatsu, Masatoshi; Yamamoto, Naoki; Noda, Yu; Nomoto, Yuka; Honda, Takashi; Takikawa, Noriyasu; Sakoda, Yoshihiro; Kida, Hiroshi

doi:10.1016/j.vetmic.2013.01.041

Cited by 19 publications

(19 citation statements)

References 19 publications

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“…Recently, Shichinohe et al reported that compared to a vaccine prepared from low pathogenicity H5 avian influenza virus, a vaccine made by a reverse genetics-based strategy similar to ours provided better protection in terms of virus secretion [8]. This vaccine strategy provides a good quantity of a vaccine antigen and proper matching between a vaccine antigen and a circulating strain, resulting in better vaccine efficacy and allowing a DIVA strategy when a particular circulating strain is targeted.…”

Section: Discussionmentioning

confidence: 99%

Application of Reverse Genetics for Producing Attenuated Vaccine Strains against Highly Pathogenic Avian Influenza Viruses

Uchida

Takemae

Saito

2014

The Journal of Veterinary Medical Science

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In this study, reverse genetics was applied to produce vaccine candidate strains against highly pathogenic avian influenza viruses (HPAIVs) of the H5N1 subtype. The H5 subtype vaccine strains were generated by a reverse genetics method in a biosafety level 2 facility. The strain contained the HA gene from the H5N1 subtype HPAIV attenuated by genetic modification at the cleavage site, the NA gene derived from the H5N1 subtype HPAI or the H5N3 subtype of avian influenza virus and internal genes from A/Puerto Rico/8/34. Vaccination with an inactivated recombinant virus with oil-emulsion completely protected chickens from a homologous viral challenge with a 640 HAU or 3,200 HAU/vaccination dose. Vaccination with a higher dose of antigen, 3,200 HAU, was effective at increasing survival and efficiently reduced viral shedding even when challenged by a virus of a different HA clade. The feasibility of differentiation of infected from vaccinated animals (DIVA) was demonstrated against a challenge with H5N1 HPAIVs when the recombinant H5N3 subtype viruses were used as the antigens of the vaccine. Our study demonstrated that the use of reverse genetics would be an option to promptly produce an inactivated vaccine with better matching of antigenicity to a circulating strain.

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Section: Discussionmentioning

confidence: 99%

Application of Reverse Genetics for Producing Attenuated Vaccine Strains against Highly Pathogenic Avian Influenza Viruses

Uchida

Takemae

Saito

2014

The Journal of Veterinary Medical Science

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“…(10) NVAL reviews the results of the suitability tests of the candidate vaccine strains. (11) The selection committee reviews the results of the suitability tests of the candidate vaccine strains and selects the most appropriate vaccine strain. (12) The Director of NVAL reports the most appropriate vaccine strains to the Director of Animal Products Safety Division.…”

Section: Vaccine Strains Selection Committee For Veterinary Influenzamentioning

confidence: 99%

“…[5]]. It also had sufficient efficacy against field strains [11]. Therefore, based on considerations of production costs and production efficiency, A/duck/ Hokkaido/Vac-3/2007(H5N1) was added to the strains of stockpiled vaccines [12].…”

Section: Vaccine Strains Selection Committee For Veterinary Influenzamentioning

confidence: 99%

Introduction of an update system for vaccine strains of veterinary influenza vaccines in Japan

Gamoh

Nakamura

2015

Biologicals

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“…A variety of approaches have been used to develop vaccines for chickens and ducks, including reverse genetic expression of H5 and N1, DNA vectors expressing H5, infectious laryngotracheitis virus expressing H5, nonpathogenic H5-subtype influenza virus, plantexpressed H5, Herpes virus of turkeys expressing H5, fowlpox virus expressing H5, Newcastle disease virus expressing H5, and baculovirus virus-like particles expressing H5 (6,8,13,25,27,31,42).…”

Section: Introductionmentioning

confidence: 99%

Protective Efficacy of a Single Dose of Baculovirus Hemagglutinin-Based Vaccine in Chickens and Ducks Against Homologous and Heterologous H5N1 Virus Infections

et al. 2014

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Outbreaks of the highly pathogenic H5N1 virus in poultry and humans are ongoing. Vaccination is an efficient method for prevention of H5N1 infection. Using chickens and ducks, we assessed the efficacy of a vaccine comprising H5N1 hemagglutinin (HA) protein produced in a baculovirus expression system. The immunized chickens and ducks were protected against lethal infection by H5N1 in an antigen dose-dependent manner. Complete protection against homologous challenge and partial protection against heterologous challenge were achieved in chickens immunized with 5 μg HA protein and in ducks immunized with 10 μg HA protein. The IgG antibody subtype was mainly detected in the sera and tissues, including the lungs. The neuraminidase (NA) inhibition assay was negative in immunized chickens and ducks. Our results indicated that the expressed HA protein by baculovirus was immunogenic to both chickens and ducks, and the immunized chickens and ducks were protected from the lethal infections of highly pathogenic H5N1 influenza virus, though ducks required more HA protein than chickens to be protected. Also, baculovirus HA-vaccinated poultry can be differentiated from infected poultry by NA inhibition assay.

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Potency of an inactivated influenza vaccine prepared from a non-pathogenic H5N1 virus against a challenge with antigenically drifted highly pathogenic avian influenza viruses in chickens

Cited by 19 publications

References 19 publications

Application of Reverse Genetics for Producing Attenuated Vaccine Strains against Highly Pathogenic Avian Influenza Viruses

Application of Reverse Genetics for Producing Attenuated Vaccine Strains against Highly Pathogenic Avian Influenza Viruses

Introduction of an update system for vaccine strains of veterinary influenza vaccines in Japan

Protective Efficacy of a Single Dose of Baculovirus Hemagglutinin-Based Vaccine in Chickens and Ducks Against Homologous and Heterologous H5N1 Virus Infections

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