Potency of Endo-Exopolysaccharide from Porphyridium cruentum (S.F.Gray) Nägeli as Antioxidant (DPPH) and Biological Toxicity (BSLT)

Abstract

Sources of antioxidants are abundant in nature, one of which is derived from microalgae. Microalgae species that have potentially to be developed as a producer of antioxidant compounds is Porphyridium cruentum (S. F. Gray) Nägeli. P. cruentum is a red microalga (Rhodophyceae) and has been known as polysaccharides producer (exopolysaccharide and endopolysaccharide) and as an antioxidant. This study aims to determine antioxidant activity of the extracts and biological toxicity of endo-and exopolysaccharide P. cruentum. The antioxidant activity use reduction method of free radicals (DPPH), whereas toxicity uses Brine Shrimp Lethality Test. (BSLT). The result shows that there is an antioxidant activity with IC 50 value of 50.586 5 mg ⋅ kg −1 (exopolysaccharide) and 145.998 8 mg ⋅ kg −1 (endopolysaccharide). In addition, the result indicates the toxic nature of the Artemia salina Leach. with LC 50 values of 513.175 1 mg ⋅ kg −1 (exopolysaccharide) and 521.823 3 mg ⋅ kg −1 (endopolysaccharide).Therefore, endo-and exopolysaccharide produced by P. cruentum suitable to be used as an alternative source of natural antioxidants and potential for further developed as antitumor drugs.

Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro

“…Similarly, the monosaccharide composition of PTP mainly included xylose, fucose, glucose and galactose. Fucose was not reported by Agustini & Kusmiati (2017), which may be due to the different origins of the algae. In this paper, we determined not only the monosaccharide composition but also the total sugar, sulfate contents and Mw (29.94%, 20.36%, and 4,810 kDa, respectively) and found that PTP is a complicated sulfated polysaccharide.…”

“…exhibited significant cytotoxicity against a variety of cancer cells, which meant that the polysaccharides might be a potential anticancer chemotherapeutic agent. For PTP, some references reported antioxidant (Agustini & Kusmiati, 2017), anti-obesity (Kim et al, 2016), anti-inflammatory, and immunomodulatory activities (Kim et al, 2013; Guzmán et al, 2003). A novel fatty alcohol ester isolated from PTP showed apoptotic anticancer activity (Samarakoon et al, 2014).…”

“…Few studies have addressed polysaccharides isolated from PTP. Agustini & Kusmiati (2017) extracted endo-exopolysaccharide and determined its antioxidant activity on DPPH. The composition of the polysaccharides included xylose, glucose, and galactose.…”

Peer Review #3 of "Sulfated polysaccharides from Phaeodactylum tricornutum: isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro (v0.1)"

“…exhibited significant cytotoxicity against a variety of cancer cells, which meant that the polysaccharides might be a potential anticancer chemotherapeutic agent. For Phaeodactylum tricornutum, some references reported antioxidant (Agustini et al, 2017), anti-obesity (Kim et al, 2016), anti-inflammatory and immunomodulatory activities (Kim et al, 2013;Guzmán et al, 2003). A novel fatty alcohol ester isolated from Phaeodactylum tricornutum showed apoptotic anticancer activity (Samarakoon et al, 2014).…”

“…A novel fatty alcohol ester isolated from Phaeodactylum tricornutum showed apoptotic anticancer activity (Samarakoon et al, 2014). Agustini et al(2017), which may be due to the different origins of the algae. In this paper, we determined not only the monosaccharide composition but also the total sugar, sulfate contents and Mw (29.94%, 20.36% and 4810 kDa, respectively) and found that PTP is a complicated sulfated polysaccharide.…”