Potensi Kentang Hitam Dalam Mereduksi Stres Oksidatif Dan Menghambat Proliferasi Sel Kanker Payudara McF-7

Abstract

The ethanolic extracts of flesh and peel of Coleus tuberosus were evaluated for its ability to reduce oxidative stress using cellular antioxidant activity testing on MCF-7 cells based on oxidation of 2.7-dichlorofluoresce diacetate (DCFH). Meanwhile, the antiproliferative activities was evaluated based on 3-(4.5-Dimethylthiazol-2)-2.5-diphenyltetrazolium bromide (MTT) assay in MCF-7 cells. The results showed that the ethanolic extract of flesh and peel have the ability to reduce oxidative stress and inhibit the proliferation of breast cancer cells MCF-7. The optimum concentration of ethanol extracts of peel in reducing oxidative stress was 200 μg/mL, while that of flesh was 400 μg/mL. Inhibitory concentration (IC50) of cell proliferation of MCF-7 by peel extract was 698.23±1.61 (µg/mL), and flesh extracts was 829.86±5.73 (µg/mL). The ability to reduce and inhibit the proliferation showed a dose dependent manner. There is a high correlation between cellular antioxidant and antiproliferation. The correlation of cellular antioxidant activity of the ethanolic extract of flesh Coleus tuberosus and antiproliferation was 0.93, while the correlation between cellular antioxidant activity ofthe ethanolicextract ofthe peel and anti-proliferation was 0.98. These results suggest that the ethanolic extract of flesh and peel of Coleus tuberosus can be used as a source of natural antioxidants and anti-proliferation.

Keanekaragaman dan Kekerabatan Morfologi Tanaman Kemili (Solanostemon rotundifolius) di Kabupaten Bangka

“…Kharinisa et al (2018) menyatakan bahwa tanaman kemili kurang terkenal di Indonesia, hanya dikenal dan digunakan sebagian masyarakat Jawa, Bali, dan Madura. Nugraheni et al (2013) menyatakan bahwa salah satu penelitian dalam bidang strategis nasional yaitu diversifikasi konsumen kebutuhan pangan yang berbasis sumber daya lokal dengan kegiatan eksplorasi dalam pemanfaatan tanaman non konvensional, diantaranya umbi-umbian. Karakterisasi pada tanaman kemili sangat penting untuk mencirikan dan sebagai informasi perkembangan serta pemanfaatan tumbuhan tersebut.…”

THE USE OF BLACK SOYBEAN (Glycine max L) EXTRACT AGAINST THE ACTIVITY OF LIVER ENZYMES (AST - ALT) AND ANTIOXIDANT SUPEROXIDE DISMUSTASE (SOD) OF WHITE RAT

“…(Wresdiyati et al, 2008;Rynertson et al, 2007). (Nugraheni et al, 2008). (Jawi et al, 2008;Sumardika et al, 2012).…”

“…Adanya suplementasi antioksidan pada aktivitas fisik maksimal mampu mencegah terjadinya kerusakan pada sel sehingga kadar ALT dalam darah tidak akan meningkat karena ALT tetap berada dalam sel yang utuh (Suda et al, 2003). (Nugraheni et al, 2008;Suda et al, 2003).…”

“…Suplementasi antosianin dari Aronia melanocarpa pada mencit yang diinduksi dengan cadmium chloride terbukti menurunkan kadar enzym AST, ALT, dan bilirubin (Jawi et al, 2008). (Murray et al, 2009;Nugraheni et al, 2008;Jawi et al, 2008).…”