Potent debriding ability of collagenolytic protease isolated from the hepatopancreas of the king crab Paralithodes camtschatica

Abstract

The debriding activity of the protease complex produced from the hepatopancreas of the king crab Paralithodes camtschatica was evaluated in vivo. The results clearly showed that the crab preparation had a high debriding ability. Individual proteases were isolated chromatographically from the crab complex to compare their debriding activities. All the purified proteases were active in vivo. The most active component of the complex was the isozyme C of crab collagenolytic protease, a member of the chymotrypsin-like protease class.

Recovery and Characterization of a Serine Collagenolytic Extract from Snow Crab (Chionoecetes opilio) By-products

“…The purification of marine serine collagenases typically involves an initial fractionation step with ammonium sulfate and (or) acetone, followed by chromatographic steps [8,10,[26][27][28][29][30][31]. To the authors' knowledge, only one study has involved direct acidic precipitation at a specific pH [12].…”

“…Those isolated from the fiddler crab Uca pugilator [6][7][8], king crab Paralithodes camtschatica [9][10][11], greenshore crab Carcinus maenas [12], snow crab C. opilio [13] and many other crustaceans and fish [14,15] resemble mammalian collagenases in splitting telopeptide fragments of native collagen and then slowly digesting alpha chains. This activity differs from that of bacterial collagenases, which attack over 200 positions of the collagen triple helix [10]. Unlike vertebrate and bacterial collagenases, which are metalloproteinases, marine serine collagenases do not appear to have any requirement for metal cofactors.…”

Immunohistochemical study of purulent wounds treated with King crab collagenase

“…We have already demonstrated (7) that the process of wound debridement is markedly accelerated in the presence of the erab collagenase. To investigate this phenomenon the above immunohistochemical studies were performed.…”

“…In vivo studies of crab collagenase were perfortned on purulent neerotie wounds fortned on Wistar rats (weight 180-220 g). The model wounds were produced in the following way (7): after depilation, full thickness wounds (2x2 ctn) were inflicted in the interscapular region under ether nar-cosis. Three days later the wound edges were fixed to the adjacent tissues and the erust was removed.…”

Konditionierung chronischer Wunden mittels proteolytischer Enzyme

“…Mit aus der Leber und Pankreas von Paralithodes camtschatica (Königskrabbe) isolierter Kollagenase konnte sowohl in vitro als auch in vivo bei Patienten mit Ulcera crurum ein effektives Débridement gezeigt werden [19,43]. Ein kommerziell erhältliches Präparat,das diese Kollagenasen beinhaltet, wurde bislang nicht hergestellt.…”