Potent effects of dioscin against pancreatic cancer via miR‐149‐3P‐mediated inhibition of the Akt1 signalling pathway

Si, Lingling; Xu, Lina; Yin, Lianhong; Qi, Yan; Han, Xu; Xu, Youwei; Zhao, Yanyan; Liu, Kexin; Peng, Jinyong

doi:10.1111/bph.13718

Cited by 63 publications

(54 citation statements)

References 58 publications

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“…However, the present study failed to determine whether the miR-222 described in the report by Vaksman et al (26) was miR-222-5p or miR-222-3p as no sequence related to this miRNA was provided in their report. Interestingly, a number of studies have proposed that miR-149-3p may act as a tumor suppressor in gastric, pancreatic and renal cell cancer (27)(28)(29). Considering the significant association between miR-149-3p expression and poor prognosis in patients with ovarian cancer revealed in the present study, these contradicting results may be a reflection of the heterogeneity in cancer types with different tissue origins.…”

Section: Discussioncontrasting

confidence: 78%

Characterizing the landscape of peritoneal exosomal microRNAs in patients with ovarian cancer by high‑throughput sequencing

Liu

Liao

et al. 2018

Oncol Lett

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In the present study, differentially expressed microRNAs (miRNAs) in peritoneal exosomes that were isolated from 10 patients with epithelial ovarian cancer (EOC) with metastasis in the abdominal cavity and 10 participants without cancer (NC) were identified. These differentially expressed miRNAs that were revealed by next-generation sequencing were categorized by Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of their target genes. Notably, two miRNAs that were associated with EOC-miR-149-3p and miR-222-5p-were identified. There were significant differences in expression of miR-149-3p and miR-222-5p between EOC and NC samples, and the effect of the expression level of the two miRNAs on the patient survival was identified using publicly available data from The Cancer Genome Atlas. There is an association between these two miRNAs and EOC, that was further verified by reverse transcription-quantitative polymerase chain reaction in peritoneal exosomes from 10 patients with EOC and NC participants. These results indicated that miR-149-3p and miR-222-5p might be novel biomarkers for evaluating the prognosis of patients with EOC and that these two miRNAs might have potential therapeutic values.

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Section: Discussioncontrasting

confidence: 78%

Characterizing the landscape of peritoneal exosomal microRNAs in patients with ovarian cancer by high‑throughput sequencing

Liu

Liao

et al. 2018

Oncol Lett

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“…The NRK‐52E and HK‐2 cells were plated in 96‐well plates at a density of 5 × 10 4 cells·mL −1 for 24 h and treated with various concentrations of dioscin (0, 50, 100, 200, 400, 800, 1600 and 3200 ng·mL −1 ) for 6, 12 and 24 h at 37°C, and then the cyto‐toxicity of the compound was assayed using the MTT method, as previously described (Si et al , ). In brief, MTT solution was added to the plates for 4 h incubation at 37°C, and then the formazan crystals were dissolved by 100 μL of DMSO.…”

Section: Methodsmentioning

confidence: 99%

Protective effects of dioscin against cisplatin‐induced nephrotoxicity via the microRNA‐34a/sirtuin 1 signalling pathway

Zhang

Tao

Yin

et al. 2017

British J Pharmacology

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“…Specifically, the expression of AKT1 is increased in colon cancer . Activated AKT1 promoted the invasive ability of human pancreatic cancer cells . Reversely, decreased AKT1 expression level inhibited ErbB2‐mediated breast cancer metastasis in vivo .…”

Section: Discussionmentioning

confidence: 98%

miR‐377‐5p inhibits lung cancer cell proliferation, invasion, and cell cycle progression by targeting AKT1 signaling

Liu

Shi

et al. 2018

J of Cellular Biochemistry

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Lung carcinoma is the most common type of malignant tumors globally, and its molecular mechanisms remained unclear. With the aim to investigate the effects of microRNA (miR)‐377‐5p on the cell development, invasion, metastasis, and cycle of lung carcinoma, this study was performed. We evaluated miR‐377‐5p expression levels in lung cancer tissues and cell models. Cell viability, proliferation, migration, invasion abilities, and cell cycle distribution were measured using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, crystal violet, transwell, and flow cytometry assay. Furthermore, expression levels of protein kinase B α subunit (AKT1) and proteins related to cell cycle and epithelial‐mesenchymal transition (EMT) were assessed using Western blot analysis and quantitative real‐time polymerase chain reaction. These results suggested that miR‐377‐5p was downregulated in vivo and in cell models, and miR‐377‐5p overexpression inhibited cell viability, proliferation, migration, invasion, and induced cell‐cycle arrest. In addition, as a target of miR‐377‐5p, AKT1 alleviated the decreases of cell viability, proliferation, migration, invasion, the S‐phase cells, the expression of cyclin D1, fibronectin, and vimentin, as well as the increases of the G0/G1‐phase cells, the expression of Foxo1, p27 kip1, p21 Cip1 and E‐cadherin when miR‐377‐5p overexpressed. In conclusion, miR‐377‐5p inhibited cell development and regulated cell cycle distribution and EMT by targeting AKT1, which provided a theoretical basis for further study of lung carcinoma therapeutics.

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Potent effects of dioscin against pancreatic cancer via miR‐149‐3P‐mediated inhibition of the Akt1 signalling pathway

Abstract: Collectively, these findings confirmed the potent effects of dioscin against pancreatic cancer and also provided novel insights into the mechanisms of the compound as a potential candidate for the treatment of pancreatic cancer.

Cited by 63 publications

References 58 publications

Characterizing the landscape of peritoneal exosomal microRNAs in patients with ovarian cancer by high‑throughput sequencing

Characterizing the landscape of peritoneal exosomal microRNAs in patients with ovarian cancer by high‑throughput sequencing

Protective effects of dioscin against cisplatin‐induced nephrotoxicity via the microRNA‐34a/sirtuin 1 signalling pathway

miR‐377‐5p inhibits lung cancer cell proliferation, invasion, and cell cycle progression by targeting AKT1 signaling

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