Potential Anti-Skin Aging Effect of (-)-Catechin Isolated from the Root Bark of Ulmus davidiana var. japonica in Tumor Necrosis Factor-α-Stimulated Normal Human Dermal Fibroblasts

Abstract

Reactive oxygen species (ROS) are generated during skin aging, including intrinsic (chronologic aging) and extrinsic aging (photoaging). Therefore, antioxidants that inhibit ROS generation can delay skin aging. In this study, we evaluated the potential anti-skin aging effect of (-)-phenolic compounds isolated from the root bark of Ulmus davidiana var. japonica. We preferentially investigated the possible preventive effects of isolates against the degradation of skin extracellular matrix. Among the isolates, (-)-catechin suppressed the activity of collagenase MMP-1, and reversed the degradation of collagen induced by tumor necrosis factor-α (TNF-α) in normal human dermal fibroblast. This action mechanism of (-)-catechin was validated by the suppression of tumor necrosis factor-α-induced accumulation of ROS and activation of mitogen-activated protein kinases, protein kinase B (Akt), and cyclooxygenase-2 (COX-2). The proinflammatory cytokines upregulate inflammatory reactions, and ultimately promote aging-related reactions. In this milieu, we demonstrated that (-)-catechin decreased the expression and secretion of proinflammatory cytokines, including interleukin (IL)-1β and IL-6. In conclusion, (-)-catechin is a candidate to ameliorate both intrinsic and extrinsic skin aging.

Osteoprotective Roles of Green Tea Catechins

“…EGCG possesses the most potent antioxidant and free radical scavenging abilities [ 39 , 40 ]. Thus, many benefits of green tea come from the free radical scavenging activity and antioxidant effects of catechins [ 36 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ].…”

Preventive Effects of Anthraquinones Isolated from an Endophytic Fungus, Colletotrichum sp. JS-0367 in Tumor Necrosis Factor-α-Stimulated Damage of Human Dermal Fibroblasts

“…Production of PGE 2 , MMP-1, procollagen I α1 (COLIA1), IL-1β, IL-6, and IL-8 in the supernatants of cells were measured using an enzyme-linked immunosorbent assay (ELISA) kit [ 41 ]. Briefly, the cells were seeded in a 48-well plate (4 × 10 4 cells/well) and incubated in serum-free medium for 24 h. The serum-starved HDFs were exposed to 50 and 100 µM of the test compounds for 1 h, and then to 20 ng/mL TNF-α for 12 h (IL-1β, IL-6, and IL-8) and 24 h (PGE 2 , MMP-1, and COLIA1).…”

“…The mRNA expression of cells was determined using quantitative real-time polymerase chain reaction (qRT-PCR) [ 41 ]. Briefly, the cells were seeded in a 6-well plate (3 × 10 5 cells/well) and incubated for 24 h. The cells were continuously starved in serum-free medium for 24 h. Thereafter, the HDFs were exposed to 50 and 100 µM compound 1 for 1 h, and then with 20 ng/mL TNF-α for 4 h (IL-1β, IL-6, and IL-8) and 24 h (PGE 2 , MMP-1, and COLIA1).…”

Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis

“…(−)-Epigallocatechin 3-gallate (EGCG), the major bioactive component of green tea catechins, has gained interest in OA research because of its potent anti-inflammatory and antioxidative properties [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. The chondroprotective effect of EGCG has been widely investigated using human articular chondrocytes [ 22 , 23 , 24 ], animal articular chondrocytes [ 25 , 26 ], bovine cartilage explants [ 27 ], and surgically induced OA animal models [ 28 , 29 , 30 ].…”