Potential antidepressant constituents of Nigella sativa seeds

Abstract

Background:Nigella sativa Linn. is well known seed in the Middle East, Asia, and the Far East as a natural remedy for many ailments and as a flavoring agent proclaimed medicinal usage dating back to the ancient Egyptians, Greeks, and Romans. An authentic saying of the Prophet Muhammad (Peace Be Upon Him) about black seed is also quoted in Al-Bukhari.Objective:This study was carried out to evaluate the antidepressant effect and isolate the potential antidepressant constituents of the polar extract of N. sativa seeds.Materials and Methods:The antidepressant effect was evaluated through the immobility duration in tail suspension and forced swim tests (FSTs). Albino mice were orally treated with N. sativa polar extract and its RP-18 column chromatography fractions (50 and 100 mg/kg,).Results:The polar extract and two of its sub-fractions were significantly able to decrease the immobility time of mice when subjected to both tail suspension and FSTs, the effects are comparable to standard drug (Sertraline, 5 mg/kg). However, these treatments did not affect the number of crossings and rearing in the open field test. Phytochemical investigation of the two active fractions led to the isolation of quercetin-3-O-α-L-rhamnopyranoside 1, quercetin-7-O-β-D-gluco- pyranoside 2, tauroside E 3, and sapindoside B as the potential antidepressant constituents.SUMMARY Phytochemical and biological evaluation the antidepressant constituents in Nigella sativa using the tail suspension and forced swim methods afforded the isolation and identification of quercetin-3-O-α-L rhamnopyranoside, quercetin-7-O-β-D gluco pyranoside, tauroside E, and sapindoside B as the potential antidepressant constituents in the polar extract of N. sativa. The isolated compounds were identified through extensive NMR analysis (1D, 2D, ESI MS). Abbreviations used: TST: Tail suspension test, FST: Forced swim test, OFT: An Open field test

Antioxidant-Rich Woodfordia fruticosa Leaf Extract Alleviates Depressive-Like Behaviors and Impede Hyperglycemia

“…GA, a polyphenol reported to be contained in W. fruticosa leaves, demonstrated antidepressant-like effects in a mice model via modulating the function of the serotonergic and catecholaminergic system, thus raising the levels of 5-HT and catecholamine in the synaptic clefts [ 130 , 131 ]. EA has also been demonstrated to possess antidepressant-like effects in FST and TST, which is reported to be mediated by adrenergic and serotonergic systems [ 132 , 133 ].…”

Protection Against Blood-Brain Barrier Permeability as a Possible Mechanism for Protective Effects of Thymoquinone Against Sickness Behaviors Induced by Lipopolysaccharide in Rats

“…Researches have shown that the most effective treatment of NS is especially related to its constituent TQ (43,44). It has been previously reported that TQ exerts an antidepressant-like effect by inhibiting the uptake of norepinephrine, serotonin, and dopamine (45). Perveen (46).…”

Nootropic and Anti-Stress Effects of Allium Cepa Bulb and Quercetin in Male Mice

“…Additionally, immobility time has been shown to be reduced by treatment with antidepressant drugs [44]. However subsequent studies has been showed that food, foodstuff and their active bioactive component when tested on FST, decreased the immobility time and produced antidepressant like effects [6,43,45,46]. The present study has shown that A.cepa and quercetin significantly reduced the immobility time in FST after repeated administration to rats at a dosage of 200 mg/kg and 20mg/kg of body weight respectively for 14 days (Figure 1).…”