Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica

Wang, Jing; Zhang, Quanbin; Zhang, Zhongshan; Song, Houfang; Li, Pengcheng

doi:10.1016/j.ijbiomac.2009.10.015

Cited by 386 publications

(202 citation statements)

References 30 publications

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“…Its weight-average molecular weight (Da) and numberaverage molecular weight (Da) are B7000 and B5300, respectively. 16,19 Probucol tablets were purchased from Qilu Pharmaceutical (Shandong, China). L-Phenylephrine, sodium nitroprusside dehydrate, acetylcholine chloride, and N Gnitro-L-arginine methyl ester (L-NAME) were all purchased from Sigma-Aldrich.…”

Section: Methodsmentioning

confidence: 99%

“…In previous studies, we have successfully prepared and characterized a low-molecular-weight fucoidan (LMWF, 7000 Da) from L aminaria japonica that shares similar biological activities with other fucoidans. [16][17][18] In particular, LMWF has an advantage over the others in that it exerts antithrombotic effect without impacting coagulation, 19 thus it has a lower hemorrhagic risk than other fucoidans. 14,15 Therefore, we hypothesized that LMWF may protect against the endothelial and vascular disorders caused by diabetes.…”

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confidence: 99%

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Low-molecular-weight fucoidan protects endothelial function and ameliorates basal hypertension in diabetic Goto-Kakizaki rats

Cui

Zheng

Zhang

et al. 2014

Laboratory Investigation

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Endothelial dysfunction, characterized by impairment of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) bioavailability, has been implicated in diabetic cardiovascular pathogenesis. In this study, low-molecular-weight fucoidan (LMWF), which has multiple biological activities including anti-inflammatory and anti-oxidative properties, was investigated for its protective effect against endothelial dysfunction in Goto-Kakizaki type 2 diabetic rats. LMWF (50, 100, or 200 mg/kg/day) or probucol (100 mg/kg/day) were given to diabetic rats for 12 weeks. Basal blood pressure, acetylcholine-or flow-mediated relaxation of mesenteric and paw arteries, endothelium-dependent dilation of aorta, eNOS phosphorylation, and NO production were measured using laser Doppler flowmetry, force myograph, hematoxylin and eosin staining, western blot analysis, and an NO assay. We found that LMWF robustly ameliorated the basal hypertension and impairment of endothelium-dependent relaxation in the aorta, as well as mesenteric and paw arteries in diabetic rats. In addition, the reduction in eNOS phosphorylation at Ser1177, eNOS expression, and NO production because of diabetes were partially reversed by LMWF treatment. However, probucol, a lipid-modifying drug with antioxidant properties, displayed only mild effects. Moreover, LMWF induced, in a dose-dependent manner, endotheliumdependent vasodilation and eNOS phosphorylation at Ser1177 in normal aorta, and also promoted Ser1177 phosphorylation and NO synthesis in primary cultured vasoendothelial cells. Thus, these data demonstrate for the first time that fucoidan protects vasoendothelial function and reduces basal blood pressure in type 2 diabetes rats via, at least in part, preservation of eNOS function. Fucoidan is therefore a potential candidate drug for protection of endothelium in diabetic cardiovascular complications. KEYWORDS: diabetes; endothelium-dependent vasodilation; endothelial nitric oxide synthase; low-molecular-weight fucoidan; nitric oxide Diabetic patients are at high risk of endothelial dysfunction and vascular lesions, because of multiple harmful stimuli, such as hyperglycemia, hyperlipidemia, and oxidative stress. These patients are susceptible to atherosclerosis, hypertension, and peripheral vascular disease. 1,2 It is well established that endothelium-derived nitric oxide (NO) maintains vascular homeostasis and health through vasodilation and protection of vasculature from various damaging agents. [3][4][5] Loss of NO induces increased activity of proinflammatory transcription factors, such as nuclear factor kappa B, resulting in expression of leukocyte adhesion molecules and production of chemokines and cytokines, which further promote endothelial inflammation and atherosclerosis. 5,6 Therefore, the endothelial dysfunction caused by imbalance of NO bioavailability in early-stage diabetes has been implicated as a sign or a predictor of a future cardiovascular event. [6][7][8] Mechanistically, a disturbance in endothelial NO synthase (eNOS) activity, refe...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Low-molecular-weight fucoidan protects endothelial function and ameliorates basal hypertension in diabetic Goto-Kakizaki rats

Cui

Zheng

Zhang

et al. 2014

Laboratory Investigation

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“…Our results demonstrated that the components of water-soluble extracts isolated from M. pyrifera had different composition to those of U. pinnatifida, with higher fucoidan content in the latter. Fucoidans present in brown algae can be considered as potent natural antioxidants (Rupérez et al, 2002;Rocha De Souza et al, 2007;Wang et al, 2010) and their antioxidant activity depends on their structural features such as degree of sulfation, molecular weight, type of the major sugar and branching. For example, low molecular weight sulfated polysaccharides have shown better antioxidant activity than those of high molecular weight, this fact can be explained because smaller polysaccharides may incorporate into the cells more efficiently and donate protons more effectively compared to larger polysaccharides (Wijesecara et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Free radical scavenging activity of extracts from seaweeds Macrocystis pyrifera and Undaria pinnatifida: applications as functional food in the diet of prawn Artemesia longinaris

Díaz

Espino²,

Arzoz³

et al. 2017

lajar

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ABSTRACT. Water-soluble extracts from seaweeds Macrocystis pyrifera (M) and Undaria pinnatifida (U) were prepared to use the polysaccharide bioactive fraction as functional food. Chemical analysis showed that the main components of both extracts were mannitol and fucoidans. Also in both cases minor amounts of rhamnose, glucose, xylose and arabinose were determine. Content of fucose+galactose was higher in U. pinnatifida (8.7%) than in M. pyrifera (5.9%). Extracts were used as feed additive to Artemesia longinaris at inclusion levels of 1 and 2% (M1 and M2; U1 and U2), a diet without extract was used as control. Radical scavenging activities were estimated for two extracts and for midgut gland samples of experimental animals. Scavenging activity was detected in both extracts, the signal decayed to 50 and 62% in 18 min for U. pinnatifida and M. pyrifera, respectively. After 30 days of feeding, results did not show variation of survival, but growth was improved in animals fed with U1 and U2. Supplementation with 1 and 2% of U. pinnatifida showed similar quenching capacity of homogenates than those fed with M2 with a rapid 1,1-diphenylpicrylhydrazyl (DPPH) decay at 2 min of reaction. Recommended concentration for culture A. longinaris is 2%, as it is that produce the best antioxidant capacity, although the growth performance was improved also significantly only with U. pinnatifida extract.

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“…These functional polysaccharides, including fucoidan, alginates and laminarans, exhibited diverse biological characteristics, including anti-coagulant, anti-inflammatory, antivirus, antitumour and anti-oxidative activities (Li et al 2008). It was reported by Wang et al (2010) that the presence of sulphate groups in fucoidan promotes its anti-oxidative activity. The sulphate group function as electron withdrawing groups, thus increasing its anti-oxidative activity.…”

Section: Introductionmentioning

confidence: 99%

Seaweed Tea: Fucoidan-Rich Functional Food Product Development from Malaysian Brown Seaweed, Sargassum binderi

Mustapha¹,

Maskat²

2017

JSM

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Our previous studies on fucoidan from Malaysian brown seaweed, Sargassum binderi, found that it exhibited significant secondary anti-oxidative activity and showed non-toxicity. In order to exploit its health benefits, fucoidan-rich seaweed tea was developed in this study. A total of 4 different brewing time treatments were performed on Sargassum binderi at 5, 10, 15 and 20 mins (F t05 , F t10 , F t15 and F t20 , respectively

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Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica

Cited by 386 publications

References 30 publications

Low-molecular-weight fucoidan protects endothelial function and ameliorates basal hypertension in diabetic Goto-Kakizaki rats

Low-molecular-weight fucoidan protects endothelial function and ameliorates basal hypertension in diabetic Goto-Kakizaki rats

Free radical scavenging activity of extracts from seaweeds Macrocystis pyrifera and Undaria pinnatifida: applications as functional food in the diet of prawn Artemesia longinaris

Seaweed Tea: Fucoidan-Rich Functional Food Product Development from Malaysian Brown Seaweed, Sargassum binderi

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