Potential Application of Alkaline Pectinase from Bacillus subtilis SS in Pulp and Paper Industry

Abstract

Pectinase production from Bacillus subtilis SS was optimized under solid-state fermentation (5,943 U/g of dry bacterial bran). The pectinase produced was stable in neutral to alkaline pH range at 70 degrees C; therefore, the suitability of this pectinase in pulp and paper industry was investigated. The enzyme pretreatment process was optimized, and a pectinase dose of 5 IU/g of oven-dried pulp (10% consistency) at pH 9.5 temperature 70 degrees C after 150 min of treatment gave the best pretreatment to the pulp. An increase of 4.3% in brightness along with an increase of 14.8 and 65.3% in whiteness and fluorescence, respectively, whereas a 15% decrease in the yellowness of the pretreated pulp were observed. There was a 5.85% reduction in kappa number and 6.1% reduction in permanganate number along with a reduction in the chemical oxygen demand value. Significant characteristics showed by pectinase open new possibilities of application of this cellulase-free enzyme in the pulp and paper industry by reducing the negative environmental impact of chemicals apart from improving the properties of paper.

Isolation and Partial Characterization of a Bacterial Thermostable Polymethyl Galacturonase from a Newly Isolated Bacillus sp. strain BR1390

“…Hg 2+ and Zn 2+ ions significantly inhibit the PMG activity, possibly due to their blocking function on thiolgroups. Based on our results, involvement of thiolgroup in PMG active site would be a possibility that is in agreement with B. subtilis polygalacturonase (25). The enzyme was identified as a metal-dependent pectinase, which was inhibited by 5 mM EDTA.…”

Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance

“…Maximum production of exo-pectinase from A. niger in SSF DMF 45 under optimum conditions was reported to be 34.2 U/g [31]. More recently, a maximum production of 5,943 U/g of alkaline pectinase production from B. subtilis SS was achieved under SSF [8]. Improvement in process with cost reduction can play a major role in the commercial success of any technology [8].…”

“…More recently, a maximum production of 5,943 U/g of alkaline pectinase production from B. subtilis SS was achieved under SSF [8]. Improvement in process with cost reduction can play a major role in the commercial success of any technology [8]. The production of pectinases by microorganisms have been studied with several natural and purified carbon sources, and a great attempt have been done for the utilization of agroindustrial wastes like wheat bran, lemon peel, apple, and orange bagasses [32].…”

“…Pectinases can be broadly classified into acidic and alkaline groups on the basis of pH requirements for optimum enzymatic activity. For decades, alkaline pectinases have been used in many industrial and biotechnological processes, such as textile and plant fiber processing, coffee and tea fermentation, oil extraction, treatment of industrial wastewater containing pectinacious materials, purification of plant viruses, and paper manufacturing [6][7][8]. Although alkaline pectinases are used in industrial processes in crude form, their purification and the knowledge of the biochemical characteristics of these enzymes are essential for the understanding of their structure, mechanisms of action, and thermostability [9].…”

Production, Purification and Characterization of Endopolygalacturonase by Bacillus subtillus

“…PG is broadly used in food industries and help in grinding, liquefaction and mining out fruit juices [5]. In industries, Aspergillus neiger is mostly use for the profitable production of PG [6] and only some studies are existing for the manufacture of PG from bacteria [7][8][9]. Most of the pectinases produced by fungi have most favourable pH variety between acidic ranges.…”