Potential Effect of Polyphenolic-Rich Fractions of Corn Silk on Protecting Endothelial Cells against High Glucose Damage Using In Vitro and In Vivo Approaches

Abstract

Endothelial cell dysfunction is considered to be one of the major causes of vascular complications in diabetes. Polyphenols are known as potent antioxidants that can contribute to the prevention of diabetes. Corn silk has been reported to contain polyphenols and has been used in folk medicine in China for the treatment of diabetes. The present study aims to investigate the potential protective role of the phenolic-rich fraction of corn silk (PRF) against injuries to vascular endothelial cells under high glucose conditions in vitro and in vivo. The protective effect of PRF from high glucose toxicity was investigated using human umbilical vein endothelial cells (HUVECs). The protective effect of PRF was subsequently evaluated by using in vivo methods in streptozotocin (STZ)-induced diabetic rats. Results showed that the PRF significantly reduced the cytotoxicity of glucose by restoring cell viability in a dose-dependent manner. PRF was also able to prevent the histological changes in the aorta of STZ-induced diabetic rats. Results suggested that PRF might have a beneficial effect on diabetic patients and may help to prevent the development and progression of diabetic complications such as diabetic nephropathy and atherosclerosis.

By‐Products of Zea mays L.: A Promising Source of Medicinal Properties with Phytochemistry and Pharmacological Activities: A Comprehensive Review

“…[96] Besides, the phenolic-rich fraction of CS protects the endothelial layer from high glucose by restoring cell viability dose-dependent manner and preventing histological changes in the aorta of STZinduced rats. [97] Hence, in vivo or in vitro studies, CS polysaccharides exhibited anti-diabetic effects, and total flavonoids, water extract, methanol extract, 95 % EtOH extract, and the phenolic-rich fraction of CS were also critical contributors to anti-diabetic activities.…”

“…The results showed that both 300 mg/kg and 500 mg/kg CSFs have significantly reduced weight loss, water consumption, and especially blood glucose concentration in diabetic mice, suggesting that CSFs have a potential therapeutic effect on diabetes [96] . Besides, the phenolic‐rich fraction of CS protects the endothelial layer from high glucose by restoring cell viability dose‐dependent manner and preventing histological changes in the aorta of STZ‐induced rats [97] . Hence, in vivo or in vitro studies, CS polysaccharides exhibited anti‐diabetic effects, and total flavonoids, water extract, methanol extract, 95 %EtOH extract, and the phenolic‐rich fraction of CS were also critical contributors to anti‐diabetic activities.…”

Current State of Research in the Field of Chemical Composition and Pharmacological Effects of Zea Maydis Styli Cum Stigmatis

“…Протективный эффект КСР исследовали in vitro с использованием эндотелиальных клеток пупочной вены человека, а в последствии in vivo у крыс с диабетом, индуцированным стрептозоцином. Показано, что фенольная фракция КСР может оказывать положительный эффект на пациентов с диабетом и играть значимую роль в предотвращении развития и прогрессирования диабетических осложнений, таких как диабетическая нефропатия и атеросклероз [46].…”

Extraction Optimization, Preliminary Identification, and Bioactivities in Corn Silk

“…Corn silk, the dried style and stigma of Zea mays L., is a wellknown traditional Chinese medicine which has been widely used as tea [1,2], herb medicine [3][4][5], and food ingredients [6,7]. Corn silk contains rich favonoids [5,8,9], polysaccharides [10,11], and terpenoids [12,13], which possess a variety of biological activities including antioxidant [9], hypoglycemic [6,14], anticancer [4,15], and antibacterial properties [16][17][18].…”