Potential enterovirulence and antimicrobial resistance in Escherichia coli isolates from aquatic environments in Rio de Janeiro, Brazil

Rebello, Raquel Costa de Luca; Régua-Mangia, Adriana Hamond

doi:10.1016/j.scitotenv.2014.04.040

Cited by 24 publications

(9 citation statements)

References 43 publications

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“…In this study, ETEC 9.91% was the most commonly detected in all effluents purchased at the sites of study. In Brazil, a similar study of the enterovirulence potential of E. coli isolates from aquatic environments in Rio de Janeiro has shown that ETEC was present in 5% of agricultural wastewaters [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Bako

Kagambèga

Traoré

et al. 2017

IJERPH

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Cattle farming can promote diarrheal disease transmission through waste, effluents or cattle fecal matter. The study aims to characterize the diarrheagenic Escherichia coli (DEC) isolated from cattle feces, manure in the composting process and slurry, collected from four cattle markets in Ouagadougou. A total of 585 samples (340 cattle feces, 200 slurries and 45 manures in the composting process) were collected from the four cattle markets between May 2015 and May 2016. A multiplex Polymerase Chain Reaction (PCR), namely 16-plex PCR, was used to screen simultaneously the virulence genes specific for shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC). DEC was detected in 10.76% of samples. ETEC was the most prevalent (9.91%). STEC and EAEC have been observed with the same rate (0.51%). ETEC were detected in 12.64% of cattle feces, in 6.66% of manure in the composting process and in 5% of slurry. STEC were detected in 0.58% of cattle feces and in 2.22% of manure in the composting process. EAEC was detected only in 1% of slurry and in 2.22% of manure in the composting process. ETEC strains were identified based on estIa gene and/or estIb gene and/or elt gene amplification. Of the 58 ETEC, 10.34% contained astA, 17.24% contained elt, 3.44% contained estIa and 79.31% contained estIb. The two positive EAEC strains contained only the aggR gene, and the third was positive only for the pic gene. The results show that effluent from cattle markets could contribute to the spreading of DEC in the environment in Burkina Faso.

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Section: Discussionmentioning

confidence: 99%

Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Bako

Kagambèga

Traoré

et al. 2017

IJERPH

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“…However, of greater concern is the increased risk that the particularlyresistant bacteria, associated with mobile genetic elements, may heighten the likelihood that ARGs and virulence factors are spread simultaneously, which could lead to the emergence of new pathogens, since resistant bacteria develop rapidly (Ramírez Castillo et al, 2013). In recent years, although substantial research has been carried out in other countries, such as China, India, Poland, and Brazil, regarding MDR bacteria and the development of resistance mechanisms in aquatic systems (Diwan et al, 2010;Korzeniewska and Harnisz, 2013;Rebello and Regua-Mangia, 2014;Xu et al, 2016), there are few studies available concerning the prevalence of MDR enteropathogenic bacteria in aquatic environments in Malaysia. This is despite the fact that the microbiological qualities of river water sources in other countries have been reported (Hamelin et al, 2007;Huang et al, 2016;Olaniran et al, 2009;Ramírez Castillo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Unlike other microorganisms, E. coli is able to acquire resistance easily and has been recognized as a contributor to the dissemination of ARGs in natural environments (Alhaj et al, 2007;Kappell et al, 2015). Furthermore, E. coli is a health threat in some tropical and subtropical areas, and is one of the leading causes of diseases and death in humans and animals (Rebello and Regua-Mangia, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Hara

Yusaimi

Zulkeflle

et al. 2018

J. Gen. Appl. Microbiol.

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The emergence of antibiotic resistance among multidrug-resistant (MDR) microbes is of growing concern, and threatens public health globally. A total of 129 Escherichia coli isolates were recovered from lowland aqueous environments near hospitals and medical service centers in the vicinity of Kuala Lumpur, Malaysia. Among the eleven antibacterial agents tested, the isolates were highly resistant to trimethoprim-sulfamethoxazole (83.7%) and nalidixic acid (71.3%) and moderately resistant to ampicillin and chloramphenicol (66.7%), tetracycline (65.1%), fosfomycin (57.4%), cefotaxime (57.4%), and ciprofloxacin (57.4%), while low resistance levels were found with aminoglycosides (kanamycin, 22.5%; gentamicin, 21.7%). The presence of relevant resistance determinants was evaluated, and the genotypic resistance determinants were as follows: sulfonamides (sulI, sulII, and sulIII), trimethoprim (dfrA1 and dfrA5), quinolones (qnrS), β-lactams (ampC and blaCTX-M), chloramphenicol (cmlA1 and cat2), tetracycline (tetA and tetM), fosfomycin (fosA and fosA3), and aminoglycosides (aphA1 and aacC2). Our data suggest that multidrug-resistant E. coli strains are ubiquitous in the aquatic systems of tropical countries and indicate that hospital wastewater may contribute to this phenomenon.

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“…In our study, the MDR phenotypes and multidrug-resistance to up to four antimicrobials were observed among ETEC isolates originated from asymptomatic children, which reinforces this observation. RAPD typing confirms the high variability among ETEC isolates thus characterizing this bacterial population as being epidemiologically unrelated [15] [37].…”

Section: Discussionmentioning

confidence: 72%

Brazilian Indigenous Children as Carriers of Diarrheagenic <i>Escherichia coli</i> Pathotypes

Coelho¹,

Gomes²,

Vieira³

et al. 2018

AID

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Introduction: Diarrheagenic Escherichia coli (DEC) is a relevant cause of diarrhea, particularly among infants and young children in developing countries. Methodology: We compared the frequency, antimicrobial resistance, adherence, enterovirulence and genetic diversity of DEC isolates from Guarani indigenous population under five living in distinct villages in Brazil. Results: Of the 314 E. coli isolates from 57 children, with and without diarrhea, 15% (48/314) were classified in DEC categories: aEPEC (56%, 27/48), EAEC (35%, 17/48) and ETEC (8%, 4/48). ETEC belonged to plylogroup A, EAEC to groups A, B1, B2 and D, and aEPEC to phylogroups A, B1, and B2. EAEC exhibited the aggregative adherence phenotype while ETEC and aEPEC the aggregative and undefined patterns. Multidrug-resistance was detected in aEPEC, ETEC and EAEC while extensive drug-resistance was found in EAEC and aEPEC. RAPD typing revealed a genetically diverse bacterial population. Conclusion: This is the first report regarding aspects of DEC in an indigenous Brazilian population, showing that Guarani children are DEC carriers and that antimicrobial resistance at high levels is widely disseminated among these enteropathogens.

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Potential enterovirulence and antimicrobial resistance in Escherichia coli isolates from aquatic environments in Rio de Janeiro, Brazil

Cited by 24 publications

References 43 publications

Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Brazilian Indigenous Children as Carriers of Diarrheagenic <i>Escherichia coli</i> Pathotypes

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Potential enterovirulence and antimicrobial resistance in Escherichia coli isolates from aquatic environments in Rio de Janeiro, Brazil

Cited by 24 publications

References 43 publications

Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Molecular characterization of multi-drug resistant <i>Escherichia coli</i> isolates from tropical environments in Southeast Asia

Brazilian Indigenous Children as Carriers of Diarrheagenic &lt;i&gt;Escherichia coli&lt;/i&gt; Pathotypes

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Brazilian Indigenous Children as Carriers of Diarrheagenic <i>Escherichia coli</i> Pathotypes