Potential hazards of bisphenol A exposure to semen quality and sperm DNA integrity among infertile men

Omran, Ghada Ali; Gaber, Hisham Diab; Mostafa, Nashwa; Abdel-Gaber, Reham Maher; Salah, Emadeldeen Ali

doi:10.1016/j.reprotox.2018.08.010

Cited by 61 publications

(36 citation statements)

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“…Our results are significantly higher than the most recent Italian reference values for phthalates [39], expressed in µg/g creatinine, for MnBP (Reference value 95th percentile (RV 95 ) 4.8 vs 31.4 mean), MBzP (RV 95 3.0 vs. 4.4 mean) and molar sum of DEHP metabolites (RV 95 20.21 vs. 82.2). This difference is particularly important for the MnBP, being our mean value about eight time the reference value.…”

Section: Discussioncontrasting

confidence: 85%

“…This difference is particularly important for the MnBP, being our mean value about eight time the reference value. The comparison with other published study presents lower differences but levels were determined in other geographical area, from five to twenty years ago and a comparison would be less accurate [39].…”

Section: Discussionmentioning

confidence: 56%

“…In an epidemiological survey on 215 young health adults, the urinary BPA levels were significantly and inversely associated with sperm concentration and total sperm count [38]. In recent surveys [39,40] sperm concentration, morphology and motility were negatively associated with BPA urinary levels.…”

mentioning

confidence: 92%

“…Also, the possible molecular mechanism of MnBP was investigated in vitro and even at low dose, an increased Sertoli cells proliferation emerged [28].Among other chemicals, evidence from previous studies showed that also bisphenol A (BPA), a precursor to important plastics like polycarbonates and epoxy resins, could interact with the endocrine system [29,30] and semen quality as well [31,32] even if the present evidence is controversial.According to Lassen et al [33] the association between BPA levels and reproductive hormones would support an anti-androgenic effect with deleterious consequences on motile spermatozoa (−6.7%, confidence interval-CI −11.76/1.63 95%), data confirmed also by Radwan et al [34].BPA induced, in in vivo studies, testicular dysfunction and alteration of Sertoli cell viability [35], testicular apoptosis in male rats [36]; after BPA exposure spermatogenic cells were disorganized and degenerated, spermatids had fragmented pyknotic nuclei and a significant decrease in the rate of proliferation of germ cells was present [37].In an epidemiological survey on 215 young health adults, the urinary BPA levels were significantly and inversely associated with sperm concentration and total sperm count [38]. In recent surveys [39,40] sperm concentration, morphology and motility were negatively associated with BPA urinary levels.…”

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confidence: 99%

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Cross Sectional Study on Exposure to BPA and Phthalates and Semen Parameters in Men Attending a Fertility Center

Caporossi

Alteri

Campo

et al. 2020

IJERPH

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Among the possible risk factors for male reproduction, exposure to phthalates and alkylphenols is widely documented. This study evaluated the possible association between chemical exposure and the quality of the seminal fluid of 105 subjects in a fertility clinic. The urinary levels of seven phthalate metabolites (monoethylphthalate, MEP; monobenzylphthalate, MBzP; mono n-butylphthalate, MnBP; mono-(2-ethylhexyl) phthalate, MEHP; mono(2-ethyl-5-hydroxyhexyl) phthalate, MEHHP; mono-n-octylphthalate, MnOP; mono-isononylphthalate, MiNP) and bisphenol A (BPA), were analysed by high performance liquid chromatography/tandem mass spectrometry HPLC/MS/MS. The regression analysis showed that the semen volume was positively associated with MnBP, MnOP and BPA levels while was negatively associated with MiNP levels. The sperm concentration had a significant inverse relationship with MEP levels. A negative association was found between the use of plastic containers for food storage (p = 0.037) and semen volume (3.06 vs. 2.30 mL as average values, never vs daily). A significant positive correlation emerged (p < 0.005) between the consumption of canned food and the levels of BPA (2.81 vs. 0.14 µg/g creat as average values, daily vs. never) and between the use of perfumes and levels of MEP (389.86 vs. 48.68 µg/g creat, as average values, daily vs. never). No further statistically significant associations were found, even considering the working activity. Some evidence emerged about the possible link between exposure and seminal fluid quality: further case/control or prospective studies will allow us to confirm this causality hypothesis.

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Section: Discussioncontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 56%

mentioning

confidence: 92%

mentioning

confidence: 99%

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Cross Sectional Study on Exposure to BPA and Phthalates and Semen Parameters in Men Attending a Fertility Center

Caporossi

Alteri

Campo

et al. 2020

IJERPH

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“…It has been shown that DNA damage could reduce the success of in vitro fertilization (IVF) (Wdowiak et al ., ) and intracytoplasmic sperm injection (ICSI) (Avendaño et al ., ), weakens pregnancy (Carlini et al ., ), and even affects the offspring's health (Jin et al ., ). Comparative studies of spermatozoa from clinical patients and laboratory animals found that BPA may be associated with declined semen quality and increased sperm DNA damage (Toyama & Yuasa, ; Meeker et al ., ; Vitku et al ., ; Fawzy et al ., ; Omran et al ., ). However, the underlying mechanism for the impacts of BPA on sperm DNA damage is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Effects of bisphenol A exposure on DNA integrity and protamination of mouse spermatozoa

et al. 2019

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Background Bisphenol A is widely used in the manufacture of polycarbonate plastics and has caused increasing concern over its potential adverse impacts on spermatogenesis. However, the effect of bisphenol A on spermiogenesis is yet to be explored. Objectives To evaluate whether bisphenol A has adverse effects on DNA integrity and protamination of spermatogenic cell. Materials and methods Newborn male mice were subcutaneously injected with bisphenol A (0.1, 5 mg/kg body weight, n = 15) or coin oil (control group, n = 20) daily from post‐natal day 1 until 35. At post‐natal day 70, epididymis caudal spermatozoa and testes were collected. Sperm count, sperm motility, and sperm morphology were analyzed. The sperm chromatin structure assay was performed to examine the sperm DNA fragmentation. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method was used to assess apoptosis of spermatogenic cells. The ultrastructural features of testicular sections were examined under a transmission electron microscope. Western blot and RT‐PCR were used to detect the expression levels of transition protein (Tnp) 1 and Tnp2, protamine (Prm) 1 and Prm2 protein, and mRNA in mice testes. Results Bisphenol A significantly reduced sperm counts, impaired sperm motility, and increased the percentage of malformed spermatozoa. Poor sperm chromatin integrity and increased TUNEL‐positive spermatogenic cells were also observed in mice exposed to bisphenol A. Ultrastructural analysis of testes showed that bisphenol A exposure caused incomplete chromatin condensation, retention of residual cytoplasm, and abnormal acrosome formation. In addition, the relative expression levels of Tnp2 and Prm2 in mice testes decreased significantly in bisphenol A groups. Discussion and conclusion Our findings identified that neonatal bisphenol A exposure may negatively contribute to the sperm quality in adult mice. Mechanistically, we showed that bisphenol A reduced sperm chromatin integrity along with increased DNA damage, which may be due to poor protamination of spermatozoa.

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Environmental Factors

Leisegang¹,

Henkel²

2020

Male Infertility

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Potential hazards of bisphenol A exposure to semen quality and sperm DNA integrity among infertile men

Cited by 61 publications

References 50 publications

Cross Sectional Study on Exposure to BPA and Phthalates and Semen Parameters in Men Attending a Fertility Center

Cross Sectional Study on Exposure to BPA and Phthalates and Semen Parameters in Men Attending a Fertility Center

Effects of bisphenol A exposure on DNA integrity and protamination of mouse spermatozoa

Environmental Factors

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