Potential Health Implication of in Vitro Human Low-Density Lipoprotein–Vitamin E Oxidation Modulation by Polyphenols Derived from Côte d’Ivoire’s Oil Palm Species

Abstract

Antioxidant activities of polyphenolic compounds extracted (PPEs) from ripe fruits of oil palms are investigated by studying their in vitro effects on human low-density lipoprotein (LDL) oxidation. Four oil palm species ( Elaeis guineensis ) are issued from the National Centre of Agronomic Research of Côte d'Ivoire, of which two are parental varieties (HP1 and HP2), while the other two are crossing varieties (HP3 and HP4). The main identified compounds were rutin (HP3 and HP4) and caffeic and chlorogenic (5-caffeoyl quinic) acids (HP1, HP3, and HP4). The highest total phenolic content was found for HP4, while it was significantly lower for HP2. Antioxidative effects were monitored by Cu(2+)- or 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH)-induced generation of conjugated dienes (lag time and oxidation rate). The highest PPE specific antioxidant activity (SAA) values were obtained with crossing varieties (HP3 and HP4) in the copper-oxidation assay. In the AAPH-oxidation assay, SAA values were comparable for all four varieties. PPEs were effective at preventing LDL-vitamin E depletion in vitro. They could exert direct beneficial antioxidant effects on vitamin E and other antioxidants contained in food and beverages in vivo, within the gastrointestinal (GI) tract. These data could also be of particular importance for a healthier nutrition or the management of chronic diseases by a polyphenol-rich diet.

Phytochemicals and Antioxidant Capacity fromNypa fruticansWurmb. Fruit

“…Our results are in good agreement with Monde et al [30], where chlorogenic acid was reported as the major compound in oil palm fruits. In addition caffeic acid, rutin and quercetin were also detected.…”

Antioxidative Properties and Ability of Phenolic Compounds of Myrtus communis Leaves to Counteract In Vitro LDL and Phospholipid Aqueous Dispersion Oxidation

“…Total McPC concentration was estimated by Folin–Ciocalteu's assay, with absorbance monitored at 760 nm (Mondé and others ). The spectrophotometric measurement was repeated 3 times for each extract and the average data was interpolated in a gallic acid calibration curve and expressed on a dry weight basis as mg of gallic acid equivalents per g of dry weight sample (mg GAE g −1 DW).…”

“…LDL was isolated from fresh plasma of healthy human subjects, obtained from the “French Blood Establishment,” in accordance with its ethical rules, and oxidizability was monitored at 234 nm for 5 μmol L −1 Cu 2+ oxidation as previously indicated (Mondé and others ). Briefly, isolated LDL was diluted to 1 μmol apoB L −1 , added with the various McPC concentrations to be tested, and then 10‐fold diluted in oxygenated PBS at pH 7.4.…”

Antioxidant effects of extra virgin olive oil enriched by myrtle phenolic extracts on iron-mediated lipid peroxidation under intestinal conditions model

“…The polar fraction (methanolic phase) was recovered, whereas the non-polar phase (hexane) followed other methanolic extractions. The fractions obtained were combined and stored at 4°C (Kalantzakis, Blekas, Pegklidou, & Boskou, 2006).Then, total phenolic compounds concentration was estimated by FolinCiocalteu's assay, with absorbance monitored at 760 nm (Monde, Carbonneau, Michel, Lauret, Diabate, Konan, et al, 2011).The result is expressed on a dry weight basis as mg of gallic acid equivalents (GAE) per kg of olive oil (mg GAE kg -1 ).…”