Potential induction of rat muscle-derived stem cells to neural-like cells by retinoic acid

Abstract

Several recent studies have demonstrated that stem cell differentiation can be generated by derivatives of retinoic acid. In this study we chose retinoic acid (RA) for inducing neural differentiation of rat muscle-derived stem cells (rMDSCs). rMDSCs were pre-induced with 10 ng/ml basic fibroblast growth factor (bFGF) and then treated with 2 μM RA. After stimulation, RA induced rMDSCs to have a neural-like morphology after 1-7 days of in vitro differentiation. In the results of immunocytochemistry, rMDSC treated with RA showed abundant positive cells against the neuronal markers neuronal-specific enolase (NSE) and tubulin-βIII (Tuj1). Also, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-positive cells were observed, indicating oligodendrocyte lineage cells. However, positive cells against glial fibrillary acidic protein (GFAP), marker of astrocytes, were not detected. The mRNA profile of these cells included higher expression of NSE compared with those of non-treated cells in real-time PCR. From the data in this work, we suggest that rMDSCs can trans-differentiate into a neural-like phenotype under the RA conditions.

Induction of Neuronal Differentiation of Rat Muscle-Derived Stem Cells in Vitro Using Basic Fibroblast Growth Factor and Ethosuximide

“…Primary muscle-derived cells were isolated from 4-week-old female Fischer rats by a method described previously [5,6]. In brief, the major hind-limb muscles were removed and enzymatically dissociated with 0.2% collagenase type XI (Sigma, Munich, Germany), dispase (Sigma, Munich, Germany), and trypsin (Gibco BRL, Grand Island, NY, USA).…”

Chitosan-based hydrogels to induce neuronal differentiation of rat muscle-derived stem cells

“…Primary muscle-derived cells were purified as previously described in our data [7,12,13]. Briefly, the major hind-limb muscles of the rats were removed, trimmed of excess connective tissue and fat, hand-minced, and washed twice with phosphate-buffered saline (PBS).…”

“…Adult stem cells have the capacity for self-renewal and possess an inherent capacity to differentiate into many cell types such as osteoblasts, adipocytes, fibroblasts, myocytes, and neurons [4][5][6][7]. The ability of adult stem cells to differentiate into neurons holds considerable promise because of their potential to serve as an unlimited source of stem cells and replace neural stem cells for the treatment of neurodegenerative diseases.…”

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

“…Various growth factors (Jori et al 2005), such as, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) are involved in transformation and morphological differentiation of stem cells into neurons (Fu et al 2004;Rasmussen et al 2011). Embryonic stem cells can be induced to differentiate into neurons and glia cells by applying a modified medium enriched with RA or bFGF (Kim et al 2011;Mitchell et al 2003). bFGF induces the cells to express a neural phenotype, oligodendrocytes and astrocytes (Malkowski et al 2007;Sobolewski et al 2005).…”