Potential Involvement of Both Type I and Type II Mechanisms in M13 Virus Inactivation by Methylene Blue Photosensitization

Abe, Hideki; Ikebuchi, Kenji; Wagner, Stephen J.; Kuwabara, Mikinori; Kamo, Naoki; Sekiguchi, Sadayoshi

doi:10.1111/j.1751-1097.1997.tb08644.x

Cited by 26 publications

(23 citation statements)

References 25 publications

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“…The wavelength-dependent complex RIs of representative materials at two different wavelengths (514 and 633 nm) are summarized in Table 1 [23], polystyrene [24], cellulose [25], oxy-hemoglobin (HbO 2 ) [26], 5CB liquid crystal [27], ethanol [28], acetic acid [29], methanol [29], human tissue (dermis) [30], and water [31]; (b) eumelanin [32], deoxy-and oxy-hemoglobin (Hb and HbO 2 ) [33], mammalian fat [34], riboflavin [35], nile red in dioxane [36], methylene blue [37], boron subphthalocyanine chloride in benzene [38], and water [39]. two wavelengths are chosen because they are available from the most widely accessible lasers (Ar ion and He-Ne laser) in the visible range and also can provide reasonable optical dispersion for biomolecules.…”

Section: A Wavelength-dependent Ri and Molecular Informationmentioning

confidence: 99%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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The identification and quantification of specific molecules are crucial for studying the pathophysiology of cells, tissues, and organs as well as diagnosis and treatment of diseases. Recent advances in holographic microspectroscopy, based on quantitative phase imaging or optical coherence tomography techniques, show promise for label-free noninvasive optical detection and quantification of specific molecules in living cells and tissues (e.g., hemoglobin protein). To provide important insight into the potential employment of holographic spectroscopy techniques in biological research and for related practical applications, we review the principles of holographic microspectroscopy techniques and highlight recent studies.

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Section: A Wavelength-dependent Ri and Molecular Informationmentioning

confidence: 99%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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“…However, photoinactivation occurred even in the presence of sodium azide, suggesting that both type I and type II mechanisms may be involved in the M13 photoinactivation process. In the presence of quencher concentrations ranging from 3.5 to 35 mM, a sodium azide protective effect was not observed, as evidenced by increasing rates of M13 phage photoinactivation, reaching a plateau thereafter [52]. Also, the effect of singlet oxygen quenchers and of hydrogen peroxide indicated singlet oxygen as the main factor responsible for the loss of biological activity of bacteriophage M13 by rose bengal [51].…”

Section: Mechanisms Of Photodynamic Inactivationmentioning

confidence: 99%

“…The results also showed that the efficacy of T4-like phage inactivation, using the same fluence rate, was dependent on the light source used, in particular when the light is delivered at a low fluence rate. M13 phage was phototreated with 5.0 μM MB and was inactivated in an irradiation dose-dependent manner [52]. Kastury and Platz [58] showed that increasing the concentration of a PS at a fixed light dose leads to increased viral inactivation as does an increase in the total light exposure at a fixed PS concentration.…”

Section: Factors Affecting Viral Pdimentioning

confidence: 99%

“…Several bacteriophages were used in photoinactivation studies as surrogates for mammalian viruses, e.g., MS2 [44], M13 [51,52], PM2 [53], Qβ [54,55,56], PRD1 [57], λ [58,59], φ6 [60], R17 [60], Serratia phage kappa [61], T5 [62], T3 [63], T7 [57,64] and T4-like [65,66,67,68], and the results show that they are effectively photoinactivated.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Costa

Faustino

Neves

et al. 2012

Viruses

200

205

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Photodynamic inactivation (PDI) has been used to inactivate microorganisms through the use of photosensitizers. The inactivation of mammalian viruses and bacteriophages by photosensitization has been applied with success since the first decades of the last century. Due to the fact that mammalian viruses are known to pose a threat to public health and that bacteriophages are frequently used as models of mammalian viruses, it is important to know and understand the mechanisms and photodynamic procedures involved in their photoinactivation. The aim of this review is to (i) summarize the main approaches developed until now for the photodynamic inactivation of bacteriophages and mammalian viruses and, (ii) discuss and compare the present state of the art of mammalian viruses PDI with phage photoinactivation, with special focus on the most relevant mechanisms, molecular targets and factors affecting the viral inactivation process.

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“…For oxygen‐dependence experiments, an Ar bubbling method was used as previously described (19). In brief, 2 mL of M13 suspension in a screw‐cap cuvette sealed with a silicon septum was placed in a fluorospectrometer.…”

Section: Methodsmentioning

confidence: 99%

Pyrimidine Dimer Formation and Oxidative Damage in M13 Bacteriophage Inactivation by Ultraviolet C Irradiation¶

Kurosaki

Abe

Morioka

et al. 2007

Photochemistry and Photobiology

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The mechanism by which UV‐C irradiation inactivates M13 bacteriophage was studied by analyzing the M13 genome using agarose gel electrophoresis and South‐Western blotting for pyrimidine dimers. The involvement of singlet oxygen (1O2) was also investigated using azide and deuterium oxide and under deoxygenated conditions. With a decrease in M13 infectivity on irradiation, single‐stranded circular genomic DNA (sc‐DNA) was converted to Form I and Form II, which had an electrophoretic mobility between that of sc‐DNA and linear‐form DNA. However, the amount of sc‐DNA remaining was not correlated with the survival of M13. The formation of cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4) pyrimidone photoproducts ((6–4)PP) increased as a function of irradiation dose. The decrease in M13 infectivity was highly correlated with the increase in CPD and (6–4)PP, whereas no change was seen in M13 coat protein on sodium dodecyl sulfate–polyacrylamide gel electrophoresis. 8‐Oxo‐7,8‐dihydro‐2′‐deoxyguanosine did not form in the M13 genome after UV‐C irradiation. Inactivation of M13 was neither enhanced by deuterium oxide nor inhibited by azide. Deoxygenation of the M13 suspension did not affect the inactivation, indicating that 1O2 did not participate in the inactivation of M13 by UV‐C irradiation under these conditions. These results indicated that UV‐C irradiation induced not only CPD and (6–4)PP formation but also additional tertiary structural change in DNA inside the M13 virions, resulting in primary damage and a loss of infectivity. The indirect effect of UV‐C irradiation such as 1O2 production followed by oxidative damage to nucleic acids and proteins might have contributed less, if at all, to the inactivation of M13 than the direct effect of UV‐C.

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Potential Involvement of Both Type I and Type II Mechanisms in M13 Virus Inactivation by Methylene Blue Photosensitization

Cited by 26 publications

References 25 publications

Biomedical applications of holographic microspectroscopy [Invited]

Biomedical applications of holographic microspectroscopy [Invited]

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Pyrimidine Dimer Formation and Oxidative Damage in M13 Bacteriophage Inactivation by Ultraviolet C Irradiation¶

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