Potential of delphinidin-3-rutinoside extracted from Solanum melongena L. as promoter of osteoblastic MC3T3-E1 function and antagonist of oxidative damage

Casati, Lavinia; Pagani, F.; Fibiani, Marta; Scalzo, Roberto Lo; Sibilia, V.

doi:10.1007/s00394-018-1618-0

Cited by 22 publications

(29 citation statements)

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“…It is also well known that ROS can significantly affect the generation and the survival of osteoblasts and osteocytes, former osteoblasts encased in the mineralized matrix which plays a key role in sensing and bone adaptation to mechanical loading [5]. High ROS levels reduce osteoblast activity and differentiation [6] and, therefore, osteogenesis and bone mineralization [7]. In line with in vitro studies, data from experimental studies showing that antioxidants administration reduces bone loss induced by gonadectomy in mice [5], and in experimental models of Sod1 (the antioxidant gene) knock-out mice, show a reduction of bone mass which gets worse with aging [8].…”

Section: Introductionmentioning

confidence: 99%

Beneficial effects of δ-tocotrienol against oxidative stress in osteoblastic cells: studies on the mechanisms of action

et al. 2019

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Purpose Natural antioxidants are considered as promising compounds in the prevention/treatment of osteoporosis. We studied the ability of purified δ-tocotrienol (δ-TT) isolated from a commercial palm oil (Elaeis guineensis) fraction to protect osteoblast MC3T3-E1 and osteocyte MLO-Y4 cells against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage and the mechanisms involved in its protective action in MC3T3-E1. Methods MC3T3-E1 and MLO-Y4 cells were treated with δ-TT (1.25-20 µg/ml for 2 h) followed by t-BHP at 250 µM or 125 µM for 3 h, respectively. MTT test was used to measure cell viability. Apoptotic cells were stained with Hoechst-33258 dye. Intracellular ROS levels were measured by dichlorofluorescein CM-DCFA. The OPT fluorimetric assay was used to detect the reduced glutathione to oxidized glutathione ratio (GSH/GSSG) contents. Results δ-TT significantly prevented the effects of t-BHP on cell viability and apoptosis reaching a maximum protective activity at 10 and 5 µg/ml in MC3T3-E1 and MLO-Y4 cells, respectively. This protective effect was due to a reduction of intracellular ROS levels and an increase in the defense systems shown by the increase in the GSH/GSSG. GSH loss induced by an inhibitor of GSH synthesis significantly reduced the δ-TT-positive effect on ROS levels. δ-TT prevention of oxidative damage was completely removed by combined treatment with the specific inhibitors of PI3K/AKT (LY294002) and Nrf2 (ML385). Conclusions The δ-TT protective effect against oxidative damage in MC3T3-E1 cells is due to a reduction of intracellular ROS levels and an increase of the GSH/GSSG ratio, and involves an interaction between the PI3K/Akt-Nrf2 signaling pathways. Keywords Tocotrienol • Oxidative stress • MC3T3-E1 cells • MLO-Y4 cells • Signaling pathways Abbreviations δ-TT δ-Tocotrienol γ-TT γ-Tocotrienol ROS Reactive oxygen species t-BHP Tert-butyl hydroperoxide FPP Farnesylpyrophosphate GGPP Geranylgeranylpyrophosphate CM-DCFA 5(6)-Carboxy-2′:7-dichlorofluorescein diacetate GSH Glutathione GSSG Glutathione disulfide Nrf2 Nuclear factor-erythroid-2-related factor 2 PI3K/Akt Phosphatidyl-inositol-3-kinase BSO l-Buthionine-sulfoximine

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Section: Introductionmentioning

confidence: 99%

Beneficial effects of δ-tocotrienol against oxidative stress in osteoblastic cells: studies on the mechanisms of action

et al. 2019

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“…A remarkable diversity in polyphenols was found in this plant, with chlorogenic acid and the anthocyanins delphinidin 3-rutinoside (D3R) and/or nasunin (NAS) as the main types represented, especially in the peel [ 26 , 27 ]. These compounds have been reported to possess a wide spectrum of nutraceutical properties, such as antioxidant, antiproliferative, and anti-inflammatory [ 28 , 29 , 30 , 31 , 32 ]; also, chlorogenic acid has been found to possess multi-antiviral activities [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

et al. 2018

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DR2B and DR2C extracts, obtained by ethanolic maceration of peel from commercially and physiologically ripe aubergine berries, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions in host cells. The antioxidative cytoprotective effects against tBOOH-induced damage were assessed in Caco2 cells, while antiviral activity was studied in Vero cells; polyphenolic fingerprints were characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. Both samples reduced reactive oxygen species (ROS) production and exhibited scavenging and chelating properties. DR2C partly counteracted the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia; interestingly, it increased intracellular GSH levels. Furthermore, DR2C inhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins’ expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.

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“…Tulipanin‐rich extract had novel biological effects as a Glucagon‐like peptide‐1 secretagogue to help to reduce the dosages of diabetic medicines and prevent diabetes (Tani, Nishikawa, Kato, & Tsuda, 2017). Additionally, purified delphinidin‐3‐rutinoside could be a preventive agent against oxidative damage in osteoblastic cells, which suggested its potential use in the prevention/treatment of osteoporosis (Casati, Pagani, Fibiani, Lo Scalzo, & Sibilia, 2019). Majority of the activity studies of delphinidin‐3‐rutinoside consume milligram quantity.…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid‐modified countercurrent chromatographic isolation of high‐purity delphinidin‐3‐rutinoside from eggplant peel

Fan

Cao

et al. 2020

Journal of Food Science

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Delphinidin-3-rutinoside, a high-value of anthocyanin, was isolated and purified by ionic liquid (IL)-modified countercurrent chromatography (CCC) from waste peel of eggplant (Solanum melongena), one of the most common vegetables consumed all around the world. Different conventional CCC and IL-CCC solvent systems were evaluated in respect of partition coefficient (K), separation factor (α), and stationary phase retention factor (S f ) to separate polar target and other components. Basic solvent system, kind of ILs, and amount of ILs were systematically optimized by totally Ktargeted strategy, which drastically reduced the experimental effort. Finally, a novel CCC two-phase solvent system (methyl tert-butyl ether-butanol-acetonitrile-1% trifluoroacetic acid water-1-butyl-3-methylimidazolium hexafluorophosphate ([C 4 MIM][PF 6 ]) [2:4:1:5:0.2; v/v/v/v/v]) was successfully established and applied. The baseline separation of target fraction was obtained in one cycle process. The purity of delphinidin-3-rutinoside was over 99%. Moreover, the distribution behavior of different kinds of ILs in biphasic solvent system and the removal method of ILs were explored. The results showed that hydrophobic IL significantly improved the partition of polar anthocyanin in organic solvent system, thereby the separation resolution and stationary phase retention through introducing intermolecular forces. This IL-modified CCC strategy may be applied for the separation of other anthocyanins from variety of natural food resources and waste.

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Potential of delphinidin-3-rutinoside extracted from Solanum melongena L. as promoter of osteoblastic MC3T3-E1 function and antagonist of oxidative damage

Abstract: These findings indicate that D3R protects MC3T3-E1 cells from oxidative damage and suggest the potential utility of dietary D3R supplement to prevent osteoblast dysfunction in age-related osteoporosis.

Cited by 22 publications

References 63 publications

Beneficial effects of δ-tocotrienol against oxidative stress in osteoblastic cells: studies on the mechanisms of action

Beneficial effects of δ-tocotrienol against oxidative stress in osteoblastic cells: studies on the mechanisms of action

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

Ionic liquid‐modified countercurrent chromatographic isolation of high‐purity delphinidin‐3‐rutinoside from eggplant peel

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