Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines

Ramos, Altina; Prata‐Sena, Maria; Castro‐Carvalho, Bruno; Dethoup, Tida; Buttachon, Suradet; Kijjoa, Anake; Rocha, Eduardo

doi:10.1016/j.apjtm.2015.09.005

Cited by 30 publications

(28 citation statements)

References 37 publications

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“…The effects of a combinatory regimen of NS extract with Dox on the viability of HepG2, HCT116, A549, A375, MCF‐7, and U251 cells were assessed by MTT assay. The results showed that the NS extract at 100 μg/mL decreased cell viability 21%, 38%, and 43% in HepG2, A375, and HCT116 cells, respectively, while Dox decreased around 50% of cell viability in accordance with our previous work . However, a combination of the NS extract with Dox at a concentration around IC 50 did not exhibit a significant enhance of Dox's cytotoxicity in HepG2, HCT116, A375, MCF7, and U251 cells (Figure A).…”

Section: Resultssupporting

confidence: 87%

“…NS extract alone decreased cell viability without any induction of DNA damage and nuclear condensation in A549 cells. Previous data showed that the same NS extract also decreased cell proliferation in other cell lines such as HCT116, HepG2, and A375 cells with a reduction of the number of colonies and an increase of death in colon (HCT116) and liver (HepG2) cells, but without any induction of DNA damage . When combined with Dox, NS extract significantly decreased the viability of A549 cells when compared with Dox alone.…”

Section: Discussionmentioning

confidence: 85%

“…sea fan, collected from the Similan Islands coral reef, Phang Nga Province, Southern Thailand. The N siamensis (NS) crude ethyl acetate extract has been prepared as previously described . Briefly, NS was cultured for one week in potato dextrose agar and 70% of seawater.…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxic effect of NS extract in combination with Dox was assessed in six human cancer cell lines using the MTT colorimetric assay . Briefly, 0.1 × 10 6 cells/mL were plated in 96‐multiwell culture plates and after 24 hours cells were incubated with NS extract at 100 μg/mL, alone or combined with Dox (IC 50 —concentration that produce 50% inhibition of cell viability of each cell line).…”

Section: Methodsmentioning

confidence: 99%

“…Marine environment comprises a great variety of organisms and nowadays the attention has been focused on marine microorganisms, especially marine‐derived fungi due to their biological and chemical diversity . Some species of the Neosartorya genus, namely the marine‐derived Neosartorya siamensis KUFA 0017 (NS) exhibited cytotoxic activity in several cancer cell lines . In our recent work, we reported that the ethyl acetate extract of NS and some of the 14 metabolites isolated showed cytotoxic effects in cells from bowel carcinoma, hepatocellular carcinoma, and malignant melanoma …”

Section: Introductionmentioning

confidence: 99%

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Can marine‐derived fungus Neosartorya siamensis KUFA 0017 extract and its secondary metabolites enhance antitumor activity of doxorubicin? An in vitro survey unveils interactions against lung cancer cells

Ramos

Castro‐Carvalho

Prata‐Sena

et al. 2019

Environmental Toxicology

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Doxorubicin (Dox) is one of the most successful anticancer drugs in use. However, chemoresistance is one of the main limitations that patients face. Therefore, development of new strategies to improve the efficacy of Dox is needed. Marine‐derived fungi are especially promising sources of new anticancer compounds. In this work, antitumor activity of crude ethyl extract of the cultures of the marine‐derived fungus Neosartorya siamensis KUFA 0017 (NS), combined with Dox, was evaluated in six cancer cell lines. To evaluate possible mechanisms involved in the eventual improvement of Dox's cytotoxicity by NS extract, effects on DNA damage, cell death, ultrastructural modifications, and intracellular accumulation of Dox were assessed. The NS extract demonstrated a significant enhancement of Dox's cytotoxic activity in A549 cells, inducing DNA damage, cell death, and intracellular accumulation of Dox. Additionally, the cytotoxic effect of eight compounds, isolated from this extract, that is, 2,4‐dihydroxy‐3‐methylacetophenone‐(C1), nortryptoquivaline‐(C2), chevalone C‐(C3), tryptoquivaline H‐(C4), fiscalin A‐(C5), epi‐fiscalin‐C (C6), epi‐neofiscalin A‐(C7), and epi‐fiscalin A‐(C8), alone and combined with Dox was also evaluated in lung cancer cells. The cytotoxic effect of Dox was potentiated by all the isolated compounds (except C1) in A549 cells. Therefore, we concluded that NS extract potentiated cytotoxicity by inhibiting cell proliferation, increasing intracellular accumulation of Dox, and inducing cell death (possibly by an autophagic process). The isolated compounds also enhanced the activity of Dox, supporting the potential of this sort of combination. These data call for further studies to characterize drug interactions and underlying mechanisms.

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Section: Resultssupporting

confidence: 87%

Section: Discussionmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Can marine‐derived fungus Neosartorya siamensis KUFA 0017 extract and its secondary metabolites enhance antitumor activity of doxorubicin? An in vitro survey unveils interactions against lung cancer cells

Ramos

Castro‐Carvalho

Prata‐Sena

et al. 2019

Environmental Toxicology

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Cytotoxic activity of Secondary Metabolites from Marine‐derived Fungus Neosartorya siamensis in Human Cancer Cells

Prata‐Sena

Ramos

Buttachon

et al. 2016

Phytotherapy Research

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Compounds isolated from the marine sea fan-derived fungus Neosartorya siamensis (KUFA 0017), namely, 2,4-dihydroxy-3-methylacetophenon (1), chevalone C (2), nortryptoquivaline (4), tryptoquivaline H (6), tryptoquivaline F (7), fiscalin A (8), epi-fiscalin A (9), epi-neofiscalin A (11) and epi-fiscalin C (13) were tested for anti-proliferative activity by MTT assay, DNA damage induction by comet assay, and induction of cell death by nuclear condensation assay on colon HCT116, liver HepG2 and melanoma A375 cancer cell lines. Compounds 2, 4, 8, 9, 11 and 13 presented IC values ranging from 24 to 153 μM in the selected cell lines. Cell death was induced in HCT116 by compounds 2, 4 and 8. In HepG2, compounds 4, 8, 9 and 11 were able to induce significant cell death. This induction of cell death is possibly not related to genotoxicity because none of the compounds induced significant DNA damage. These results suggest that selected compounds present an interesting anti-proliferative activity and cell death induction, consequently showing potential (specifically epi-fiscalin C) as future leads for chemotherapeutic agents. Further studies on mechanisms of action should ensue. Copyright © 2016 John Wiley & Sons, Ltd.

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Exploration of in vitro cytotoxic and in ovo antiangiogenic activity of ethyl acetate extract of Penicillium oxalicum

Verma

Rai

Gupta

et al. 2023

Environmental Toxicology

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Fungal endophytes have established new paradigms in the area of biomedicine due to their ability to produce metabolites of pharmacological importance. The present study reports the in vitro cytotoxic and in ovo antiangiogenic activity of the ethyl acetate (EA) extract of Penicillium oxalicum and their chemical profiling through Gas Chromatography–Mass Spectrometry analysis. Treatment of the EA extract of P. oxalicum to the selected human breast cancer cell lines (MDA‐MB‐231 and MCF‐7) leads to the reduced glucose uptake and increased nitric oxide production suggesting the cytotoxic activity of EA extract of P. oxalicum. Our results further show that treatment of EA extract of P. oxalicum attenuates the colony number, cell migration ability and alters nuclear morphology in both the human breast cancer cell lines. Furthermore, the treatment of EA extract of P. oxalicum mediates apoptosis by increasing the expression of BAX, P21, FADD, and CASPASE‐8 genes, with increased Caspase‐3 activity. Additionally, in ovo chorioallantoic membrane (CAM) assay showed that the treatment of EA extract of P. oxalicum leads to antiangiogenic activity with perturbed formation of blood vessels. Overall, our findings suggest that the EA extract of P. oxalicum show in vitro cytotoxic and antiproliferative activity against human breast cancer cell lines, and in ovo antiangiogenic activity in CAM model.

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Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines

Cited by 30 publications

References 37 publications

Can marine‐derived fungus Neosartorya siamensis KUFA 0017 extract and its secondary metabolites enhance antitumor activity of doxorubicin? An in vitro survey unveils interactions against lung cancer cells

Can marine‐derived fungus Neosartorya siamensis KUFA 0017 extract and its secondary metabolites enhance antitumor activity of doxorubicin? An in vitro survey unveils interactions against lung cancer cells

Cytotoxic activity of Secondary Metabolites from Marine‐derived Fungus Neosartorya siamensis in Human Cancer Cells

Exploration of in vitro cytotoxic and in ovo antiangiogenic activity of ethyl acetate extract of Penicillium oxalicum

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