Potential of label-free detection in high-content-screening applications

Proll, Günther; Steinle, Lutz; Pröll, Florian; Kumpf, Michael; Moehrle, Bernd; Mehlmann, Martin; Gauglitz, Güenter

doi:10.1016/j.chroma.2007.06.022

Cited by 35 publications

(19 citation statements)

References 47 publications

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“…Label-free quantification strategies are becoming increasingly popular to compare samples . The rationale behind these methods relies on the comparison of peptide abundance as a measure for the corresponding protein between multiple LC-MS/MS analyses (Proll et al 2007). Ideally samples for label-free comparisons are run consecutively on the same LC-MS/MS setup to avoid variations due to the system setup (column properties, temperatures) and thereby allow precise reproduction of retention times.…”

Section: Protein Quantitationmentioning

confidence: 99%

Plant Protein Analysis

Malcevschi¹,

Marmiroli²

2012

Proteomic Applications in Biology

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Section: Protein Quantitationmentioning

confidence: 99%

Plant Protein Analysis

Malcevschi¹,

Marmiroli²

2012

Proteomic Applications in Biology

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“…As a laboratory method RIfS has also been evaluated for use in different experiments, for example, for bacterial infections [243], testing of biocompatibility [244], and coupling with other highly sophisticated methods {e.g., matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for the determination of binding interaction of antibiotics [245]}, in addition to being used as a bridging technology for different high-content screening approaches in the development chain of leading structures for the pharmaceutical industry or during the identification and validation processes of new biomarkers [246].…”

Section: Reflectometric Interference Spectroscopy (Rifs)mentioning

confidence: 99%

Instruments in Biotechnology and Medicine

Voss¹,

Feller²,

Beckmann³

2012

Handbook of Biophotonics

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The sections in this article are Photonic Instruments in Medicine Introduction Optical Window of Cells and Tissues Laser Light–Matter Interaction as a Function of Intensity Photochemical Treatment of Tissues Thermal Processes in Light–Matter Interaction Nonlinear Processes in Light–Matter Interaction (Not to Be Confused with Nonlinear Optics) Minimally Invasive Diagnostics – Endoscopy History Types and Components of Endoscopes Advantages and Disadvantages of Endoscopy Application Fields Combination of Standard White Light Endoscopic with Other Diagnostic Methods Some Trends in Endoscopy Noninvasive Diagnostics – Photoplethysmography Introduction Characterization of the PPG Signal Signal Acquisition Clinical Applications Summary Noninvasive Diagnostics – Ophthalmology Introduction Corneal Topography Perimetry Optical Biometry Retinal Imaging and Glaucoma Diagnostics Fluorescein Angiography Wavefront Analysis In Vitro Diagnostics – Microscopy Light Microscope Fluorescence Microscope New Fluorescence Microscope Techniques Confocal Microscopy Raman Microscopy In Vitro Diagnostics – Digital Slides and Virtual Microscopy In Vitro Diagnostics – Optical Methods in Clinical Analysis System Optical Spectroscopy of Blood and Urine Components Optical Spectroscopy of Tissues and Vessels Optical Spectroscopy of Agglutination and Precipitation – Nephelometry, Turbidimetry In Vitro Diagnostics – Blood Gas Pressure Measurements Optical Detection of p O 2 in Blood Optical Detection of p CO 2 in Blood Optical Detection of Further Monitoring Parameters in Intensive Care (Temperature, p H ) In Vitro Diagnostics – Photoacoustic Spectroscopy Therapy Introduction Laser Therapy in Ophthalmology – LASIK (Refraction Correction by Cornea Treatment) Photonic Instruments in Biotechnology Introduction Selected Analytical Methods Introduction Reflectometric Interference Spectroscopy ( RIf S ) Surface Plasmon Resonance ( SPR ) Fluorescence Methods Advanced Microscopic Techniques (Selection) Enzyme‐Linked Immunosorbent Assay ( ELISA ) Optical Biosensors, Biochips, Miniaturized Analytical Systems High‐Throughput Screening ( HTS ) Sample Preparation: Flow Injection Analysis Fermentation as a Central Process of Biotechnology General Aspects Fermentation Control Photobioreactors Optical Trap/Optical Tweezers Basic Scheme of Laser Tweezers and Micromanipulation Applications of Optical Tweezers

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“…In very simple terms, RIfS relies on partial reflection of light upon transfer between two materials with different refractive indices combined with thin-film interference effects [34]. In an assay, a portion of incident light will be reflected at each material interface, forming an interference pattern when the path length of light through a particular layer supersedes the coherence wavelength, as is often the case in the immobilized layer ( Fig.…”

Section: Reflectometric Interference Spectroscopy (Rifs)mentioning

confidence: 99%

“…A change in the height or refractive index of the immobilized layer, which occurs upon adsorption of molecules to the layer, will lead to changes in the interference pattern, which can be observed over time with a diode-array detector, for instance. Since RIfS depth of penetration into the solution is about 100-fold greater than that of SPRi [34], this technique is capable of providing more pronounced signals. Further, because RIfS relies on both the refractive index and the path length of light through the immobilized layer, temperature change-induced refraction index changes are not as much of a concern as they are with SPRi.…”

Section: Reflectometric Interference Spectroscopy (Rifs)mentioning

confidence: 99%