Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer

Abstract

Abstract. Elfiati D, Delvian, Hanum H, Susilowati A, Rachmat HH. 2021. Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer. Biodiversitas 22: 3042-3048. Phosphate-solubilizing fungi are the microbes that have the ability to dissolve insoluble phosphate and made it available for plants. Therefore, the purpose of this study was to obtain the phosphate-solubilizing fungi from peat soils. Peat soil samples were taken in a composite at a depth 0-20 cm from the peat ecosystem in Nagasaribu Village, Lintong Nihuta Sub-district, Humbang Hasundutan District, North Sumatra, Indonesia. Soil samples were isolated to obtain phosphate-solubilizing fungi using the Pikovskaya selective medium. The obtained isolates were tested for their ability to dissolve phosphate qualitatively by calculating the dissolution index values and quantitatively by calculating the available phosphorus on Pikovskaya medium by using four phosphate sources, namely Ca3(PO4)2, AlPO4, FePO4, and phosphate rock. Total of 12 isolates of phosphate-solubilizing fungi was obtained during the present study. Based on the results of qualitative and quantitative testing, all 12 isolates have the ability to release phosphate from the four tasted phosphate sources. The qualitative test obtains dissolution index values that vary from 2.55 to 4.25, while quantitatively, the isolates were able to dissolve phosphate in the value range from 17.77 ppm to 69.86 ppm. The top five fungal isolates with highest phosphate-solubilizing potential were FG5, FG8, FG9, FG11, and FG12. Based on molecular identification, these five isolates were identified as Aspergillus niger.

Phosphate-Solubilizing Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilize Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates

“…Thus, organic acids produced by PSB metabolism bound or chelate cations that bound P, therefore P solubility increases [ 44 , 45 , 46 ]. According to Mahidi [ 47 ] and Elfiati et al [ 48 ], the molecular structure of organic acids, notably the number of carboxyl and hydroxyl groups, has a significant influence on their ability to chelate metal cations. The type and position of the ligand, in addition to the acid’s strength, determine its efficacy in the dissolving process.…”

PGPR Indigenous Peat Soil as Seed Biopriming and Bioprotectant in Early Growth of Corn Plant with Fusarium sp Inoculation

“…Bacterial isolates were obtained from rhizosphere Zea mays saccharata in peatland with pH range of 3.0-4.2. This bacterium has been tested in vitro as a potential bioprotectant against corn stem rot disease caused Fusarium sp (Prasetyo and Wahyu, 2019;Elfiati et al, 2021;Hidayati et al, 2022;Purwanto et al, 2022). Additionally, this isolate also has the potential as a biofertilizer.…”

Biocontrol of wilt disease of rice seedlings incited by Fusarium oxysporum through soil application of Streptomyces chilikensis RC1830

“…Phosphate solubilization assay was carried out using modified Pikovskaya's medium. Strain RC1830 was streaked in a solid Pikovskaya's medium containing 2% (w/v) (Pandey and Gupta 2020) of various phosphorous sources such as aluminium phosphate (AlPO 4 ), calcium phosphate (Ca 3 (PO 4 ) 2 ), ferric phosphate (FePO 4 ) and incubated at 37°C (Elfiati et al 2021).…”