Potential of supercritical fluid myrtle extracts as an active ingredient and co-preservative for cosmetic and topical pharmaceutical applications

Pereira, Paula; Maurício, Elisabete Muchagato; Duarte, Maria Paula; Lima, Katelene; Fernandes, Ana Sofia; Bernardo-Gil, Gabriela; Cebola, Maria-João

doi:10.1016/j.scp.2022.100739

Cited by 10 publications

(7 citation statements)

References 54 publications

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“…These results are promising since these waste materials could be valorized using environmentally friendly and energetically favorable maceration extraction. The lower susceptibility of Gram-negative bacteria is in line with results reported for other plant extracts [26,27], including bark extracts from other species [44,45]. This difference in sensitivity may be associated with the protection of the lipopolysaccharide layer in the outer membrane of Gram-negative bacteria [26].…”

Section: Discussionsupporting

confidence: 87%

“…The cork extracts (E) were evaporated to dryness and dissolved in 50% ethanol/water solution before the antimicrobial tests. The antimicrobial activities were assayed by the agar diffusion method according to Correia et al [26] and Pereira et al [27]. Antibacterial activity of the cork extracts was assayed against both Gram-negative bacteria (Escherichia coli ATCC ® 8739 TM Microorganisms were kept frozen at −70 • C in broth containing glycerol (15% v/v).…”

Section: Antimicrobial Activitymentioning

confidence: 99%

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Maceration of Waste Cork in Binary Hydrophilic Solvents for the Production of Functional Extracts

et al. 2023

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Waste-grade cork samples of Quercus cerris were subjected to maceration extraction using 7 different solvents, including pure water (I), pure acetone (II), 75% aqueous ethanol (III), 75% aqueous methanol (IV), 75% aqueous acetone (V), 50% aqueous acetone (VI), and 25% aqueous acetone (VII). The extract yields, extract compositions, as well as antioxidant and antimicrobial activities of the extracts were analyzed. The results showed that maceration extraction was highly efficient, particularly with binary solvents resulting in up to 6% extract yield and up to 488 mg GAE/g extract total phenolic content. The extracts exhibited a variable antioxidant activity determined by DPPH and FRAP methods as well as antimicrobial activity against gram-positive bacteria and fungus determined by agar diffusion test. The CIELAB color parameters of extracts were correlated with maceration time, and the correlation was highest with pure water extracts. The FT-IR spectra of acetone-extracted cork revealed six key markers of phenolic compounds with the presence of peaks at approximately 2920 cm−1, 2850 cm−1, 1609 cm−1, 1517 cm−1, 1277 cm−1, and 1114 cm−1. The overall results suggest that the maceration of waste cork in binary solvents and pure acetone are green alternatives to conventional Soxhlet extraction for the production of polar extracts.

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Section: Discussionsupporting

confidence: 87%

Section: Antimicrobial Activitymentioning

confidence: 99%

Maceration of Waste Cork in Binary Hydrophilic Solvents for the Production of Functional Extracts

et al. 2023

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“…However, the MIC of the 2–10 kDa fraction against C. krusei was higher than 1.0 mg/mL, indicating that this particular pathogenic yeast resisted the 2–10 kDa fraction at the tested concentrations ( Table 1 ). Considering that several authors classify the antimicrobial activity of natural substances as strong inhibitors (MIC up to 0.5 mg/mL), moderate inhibitors (MIC between 0.6 and 1.5 mg/mL), and weak inhibitors (MIC above 1.6 mg/mL) [ 44 , 45 , 46 ], it is possible to confirm that the peptide fraction under study presents a strong inhibitory activity against bacteria and moderate against the C. albicans .…”

Section: Resultsmentioning

confidence: 99%

“…The conventional minimal inhibitory concentration (MIC) was determined by the broth microdilution method in 96-well microtiter plates, according to Pereira et al (2022) [ 46 ]. The peptide fraction was prepared at 2 mg/mL and a dilution of 1:2 was introduced in the first line, followed by a series of 2-fold dilutions in Sabouraud Dextrose broth (Biokar Diagnostics, Allonne, France) for yeasts ( C. albicans and C. krusei ) and in Mueller-Hinton broth (Biokar Diagnostics, Allonne, France) for the remaining bacteria.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties

Branco,

Maurício,

Costa

et al. 2023

Antibiotics

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The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism. The antimicrobial activity of the peptide fraction is evaluated against various foodborne pathogens, including Candida albicans, Candida krusei, Escherichia coli, Listeria monocytogenes, and Salmonella sp. The peptide fraction antioxidant properties are assessed using FRAP and DPPH scavenging capacity assays. Furthermore, the peptide fraction’s cytotoxicity is evaluated in colorectal carcinoma and normal colon epithelial cells while its potential as an antidiabetic agent is investigated through α-amylase and α-glucosidase inhibitory assays. The results demonstrate that the 2–10 kDa peptide fraction exhibits antimicrobial effects against all tested microorganisms, except C. krusei. The minimal inhibitory concentration for E. coli, L. monocytogenes, and Salmonella sp. remains consistently low, at 0.25 mg/mL, while C. albicans requires a higher concentration of 1.0 mg/mL. Furthermore, the peptide fraction displays antioxidant activity, as evidenced by DPPH radical scavenging activity of 81.03%, and FRAP values of 1042.50 ± 32.5 µM TE/mL at 1.0 mg/mL. The peptide fraction exhibits no cytotoxicity in both tumor and non-tumoral human cells at a concentration up to 0.3 mg/mL. Moreover, the peptide fraction presents anti-inflammatory activity, significantly reducing the expression of the TNFα gene by more than 29.7% in non-stimulated colon cells and by 50% in lipopolysaccharide-stimulated colon cells. It also inhibits the activity of the carbohydrate digestive enzymes α-amylase (IC50 of 199.3 ± 0.9 µg/mL) and α-glucosidase (IC20 of 270.6 ± 6.0 µg/mL). Overall, the findings showed that the peptide fraction exhibits antibacterial, antioxidant, anti-inflammatory, and antidiabetic activity. This study represents a step forward in the evaluation of the functional biological properties of S. cerevisiae bioactive peptides.

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“…The Ames test was carried out following OECD guideline No. 471 [ 24 ] and the ICH S2 (R1) guideline [ 29 ], according to previously published protocols [ 37 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Malú

Lima

Malmir

et al. 2022

Plants

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Dried leaves of Lannea velutina A. Rich. and Sorindeia juglandifolia (A. Rich.) Planch. ex Oliv. (family Anacardiaceae) are used in African traditional medicine. Although these medicinal plants have widespread use in the treatment of inflammatory diseases, there is no scientific data concerning their preclinical or clinical safety. This work aimed to investigate the phytochemical properties of the leaves of both species using HPLC-UV/DAD, as well as the in vivo oral repeated-dose toxicity of 70% hydroethanolic leaf extract of S. juglandifolia and the in vitro genotoxicity of 70% hydroethanolic leaf extracts of L. velutina and S. juglandifolia. Clinical signs of toxicity, body weight variations, and changes in food consumption, mortality, and blood biochemical parameters were monitored. Genotoxicity was assessed using the bacterial reverse mutation assay (Ames test) with and without metabolic activation, according to OECD guidelines. The obtained results showed the presence of gallic acid and anacardic acid as the main marker constituents in both species. No significant changes in general body weight or food intake were observed; small significant changes with no critical relevance were observed in the blood biochemistry of animals treated with S. juglandifolia hydroethanolic extract (50, 400, and 1000 mg/kg body weight) compared to those in the control group. No genotoxicity was observed in the bacterial reverse mutation assay with S. juglandifolia and L. velutina extracts (up to 5 mg/plate). The safety data obtained in vivo and lack of genotoxic potential in vitro points to the safe medicinal use of S. juglandifolia and L. velutina extracts.

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Potential of supercritical fluid myrtle extracts as an active ingredient and co-preservative for cosmetic and topical pharmaceutical applications

Cited by 10 publications

References 54 publications

Maceration of Waste Cork in Binary Hydrophilic Solvents for the Production of Functional Extracts

Maceration of Waste Cork in Binary Hydrophilic Solvents for the Production of Functional Extracts

Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

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