Potential Pathogenicity and Host Range of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Healthy Poultry

Bortolaia, Valeria; Larsen, Jesper; Damborg, Peter; Guardabassi, Luca

doi:10.1128/aem.02890-10

Cited by 38 publications

(26 citation statements)

References 24 publications

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“…The bla CTX-M-1 /IncI1/ST3 plasmid was previously recognized in several animal species and countries (6,8,10,27,28). However, such predominance was unexpected in this randomly tested dog population.…”

Section: Resultsmentioning

confidence: 76%

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Haenni¹,

Saras²,

Métayer³

et al. 2014

Antimicrob Agents Chemother

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bIn the community, close contacts between humans and dogs may promote the transfer of extended-spectrum beta-lactamase/ plasmidic AmpC cephalosporinase (ESBL/pAmpC) genes. Large-scale prevalence studies on ESBL/pAmpC carriage in dogs are rare, and data on ESBL/pAmpC plasmids are even more limited. Here, a considerable rate of 18.5% ESBL/pAmpC carriers was found among 368 unrelated healthy dogs in Paris, France. This prevalence is much higher than the one found in healthy humans in the same city (6%) but close to that recently reported in dogs in China (24.5%). All isolates were identified as Escherichia coli, except one Salmonella enterica and one Klebsiella pneumoniae isolate. The sequence type 131 (ST131) clone was rare (2/73 isolates). Interestingly, two plasmids (bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2) were unexpectedly highly predominant, raising the question of their successful spread. Considering that CTX-M-1 was recently found to be equally as abundant as CTX-M-15 in healthy Parisian subjects, the question of dogs being a CTX-M-1 reservoir for humans is open. Such a high prevalence of the bla CMY-2 /IncI1/ST2 plasmid may result from the use of cephalexin in veterinary medicine, as previously demonstrated experimentally. In all, our study points out healthy urban dogs as a potential source of ESBL/pAmpC genes that can further disseminate to the human community.

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Section: Resultsmentioning

confidence: 76%

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Haenni¹,

Saras²,

Métayer³

et al. 2014

Antimicrob Agents Chemother

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“…Especially, ampC-like gene, blaCMY2 was noticed in ceftriaxone-resistant E. coli isolates of animal origin ). Carriage of ESBL and AmpC-producing E. coli has been acknowledged in many species (Bortolaia et al, 2011). ESBL E. coli (O'Keefe et al, 2010) and AmpC producing E. coli (Oteo et al, 2010) are the emerging problems.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Milton¹

2015

Adv. Anim. Vet. Sci.

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| Nosocomial, or hospital-acquired, infections are well-thought-out to be the most common problem affecting hospitalized human patients as well as veterinary patients. These are of great consequence in veterinary medicine and can have considerable adverse effects on the individual patient as well as on the veterinary hospital as a whole. Different infectious agents like MRSA, Cl. difficile, MDR E. coli, Salmonella spp. and many more are of prodigious significance in causing NIs. A surveillance and control measure for these NIs in veterinary settings needs to be addressed in future. This review consolidates some Nosocomial infections and the strategy for its surveillance in veterinary practice.

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“…5 Faecal carriage of ESBL/AmpC-producing Escherichia coli in food-producing animals has been reported previously, 6 -10 and a partial genetic relatedness of genes, plasmids or host bacteria from food-producing animals and humans has been described. 6,11,12 Evidence of direct transfer was lacking. Faecal carriage in cattle has been reported previously.…”

Section: Introductionmentioning

confidence: 99%

Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach

Hordijk¹,

Mevius²,

Kant³

et al. 2013

Journal of Antimicrobial Chemotherapy

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Objectives: To assess the within-farm dynamics of extended-spectrum b-lactamase (ESBL)/AmpC-producing Escherichia coli in veal calves.Methods: Three veal-calf fattening farms were screened. Faecal samples from all calves within a compartment (109 -150 per farm) were taken upon arrival on the farm (T0) and after 3, 6, 8 and 10 weeks (T3 -T10). ESBL/AmpC genes were characterized by PCR and sequencing. Plasmids were characterized by transformation, PCR-based replicon typing and plasmid multilocus sequence typing (MLST). E. coli genotypes were analysed by MLST.Results: At T0 the prevalence of ESBL/AmpC-producing E. coli ranged from 18% to 26%. These were predominantly isolates carrying bla CTX-M-1 and bla CTX-M-15 genes, located on various plasmids and E. coli sequence types (STs). Farm 1 was negative for ESBL/AmpC-producing E. coli after T0. Farm 2 showed an increase up to 37% at T3, which subsequently decreased gradually to 0% at T10. The presence from T3 to T10 on farm 2 was mainly caused by the clonal spread of a multiresistant E. coli ST57 harbouring bla CTX-M-14 on an IncF F2:A-:B-plasmid. Farm 3 showed a gradual decrease in prevalence to 1.4% at T10, with a relative increase of the identical clonal variant as shown for farm 2. A second clonal variant found in farm 3 was a multiresistant E. coli ST10 harbouring bla CTX-M-14 on an IncK plasmid. Conclusions:The prevalence of ESBL/AmpC-producing E. coli decreased over time. A clonal spread was observed on farm 2 and farm 3, illustrative of the complex dynamics probably associated with the use of antimicrobials.

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Potential Pathogenicity and Host Range of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Healthy Poultry

Cited by 38 publications

References 24 publications

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach

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Potential Pathogenicity and Host Range of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Healthy Poultry

Cited by 38 publications

References 24 publications

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach

Contact Info

Product

Resources

About

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France