Potential proinflammatory and osteogenic effects of dicalcium silicate particles in vitro

Chen, Liangjiao; Zhu, Ping; Liu, Ruoyu; Sun, Ting; Liu, Yanjun; Longquan, Shao

doi:10.1016/j.jmbbm.2014.12.012

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…In the current study, C 2 S-coated surfaces significantly increased the expression and production of TNF-a. The effect of C 2 Scoated surfaces on TNF-a is consistent with the results of previous studies on C 2 S particles [20]. In addition, C 2 Scoated surfaces significantly increased the production of IL-6 and IL-1b, possibly due to the release of ions or particles from the coated surfaces.…”

Section: Discussionsupporting

confidence: 90%

“…In our previous study [20], C 2 S particles were shown to produce no obvious cytotoxicity in THP-1, PBMC and MG-63 cells. The cytotoxicity of C 2 S-and 45S5-coated surfaces has never been evaluated when directly cultured with macrophages.…”

Section: Discussionmentioning

confidence: 89%

“…For example, the interaction between HA particles and human monocytes causes the production of inflammatory mediators, including tumor necrosis factor alpha (TNF-a) and IL-6 [17][18][19]. In a previous study, we suggested that C 2 S particles also cause pro-inflammatory effects [20]. However, C 2 S is often used as a coating material.…”

Section: Introductionmentioning

confidence: 99%

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Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca2SiO4, C2S) particles. However, the immunological effects of C2S coatings have not been studied. C2S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C2S particles. Further, it remains unclear whether C2S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C2S coating on RAW 264.7 macrophages. C2S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C2S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested coating materials are not cytotoxic to macrophages. The C2S-coated surface stimulated macrophages to express pro-inflammatory mediators, such as TNF-α, IL-6 and IL-1β, and C2S coating caused less IL-6 but greater IL-1β production than the 45S5 coating. C2S coating have no cytotoxicity when directly cultured with macrophages. C2S and 45S5 coatings both have the potential to induce pro-inflammatory effects, and the biocompatibility of C2S is similar to that of 45S5.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 89%

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Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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“…This suggests a higher bone turnover rate in favor of bone formation. Likewise, MG-63 cells have shown similar results when exposed to hydroxyapatite and dicalcium silicate particles that are used as an implant surface coating [43]. The favorable effect of MP coating on bone formation by affecting the OPG/RANKL system should be further investigated by animal or clinical studies.…”

Section: Discussionmentioning

confidence: 92%

Effect of Multi-Phosphonate Coating of Titanium Surfaces on Osteogenic Potential

et al. 2020

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The aim of this study was to evaluate the impact of a novel multi-phosphonate (MP) coating strategy of dental implant surfaces on the expression of osteogenesis-related factors in vitro. MG-63 human osteoblast-like cells, bone marrow mesenchymal stem cells (BM-MSCs), and human periodontal ligament stem cells (hPDLSCs) were cultured separately on titanium disks with and without MP coating. Cell attachment was visualized by focal adhesion and actin cytoskeleton staining. The proliferation and gene expression of the markers related to osteogenesis and bone turnover were measured after 48 and 120 h of cell culture. Actin cytoskeleton assembly and focal adhesion were similar between test surfaces within each cell type but differed from those on tissue culture plastic (TCP). The proliferation of MG-63 cells and PDLSCs was comparable on all surfaces, while BM-MSCs showed an increase on tissue culture plastic (TCP) versus titanium. The gene expression of osteoprotegerin and receptor activator of nuclear factor-kappa B ligand was higher in MG-63 cells grown on MP-coated surfaces. At the same time, osteocalcin was decreased compared to the other surfaces. Collagen type I gene expression after 120 h was significantly lower in hPDLSCs cultivated on MP-coated surfaces. Within the limitations of this study, MP coating on titanium surfaces might have a slight beneficial effect on bone turnover in vitro.

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“…Dicalcium silicate cement is also a model system for drug release 74) . Dicalcium silicate cement has adequate biological properties and can be used as a root-end filling and pulp capping material, and exhibits good bioactivity and biocompatibility in in vitro studies 74,82–86) .…”

Section: Resultsmentioning

confidence: 99%

Calcium silicate-based cements and functional impacts of various constituents

Saghiri

Orangi

Asatourian

et al. 2017

Dental Materials Journal

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Calcium silicate-based cements have superior sealing ability, bioactivity, and marginal adaptation, which make them suitable for different dental treatment applications. However, they exhibit some drawbacks such as long setting time and poor handling characteristics. To overcome these limitations calcium silicates are engineered with various constituents to improve specific characteristics of the base material, and are the focus of this review. An electronic search of the PubMed, MEDLINE, and EMBASE via OVID databases using appropriate terms and keywords related to the use, application, and properties of calcium silicate-based cements was conducted. Two independent reviewers obtained and analyzed the full texts of the selected articles. Although the effects of various constituents and additives to the base Portland cement-like materials have been investigated, there is no one particular ingredient that stands out as being most important. Applying nanotechnology and new synthesis methods for powders most positively affected the cement properties.

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Potential proinflammatory and osteogenic effects of dicalcium silicate particles in vitro

Cited by 9 publications

References 55 publications

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Effect of Multi-Phosphonate Coating of Titanium Surfaces on Osteogenic Potential

Calcium silicate-based cements and functional impacts of various constituents

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