Tuna hand line-trolling line (HL-TR) fisheries have an essential role in Indonesia’s tuna fisheries, including Indonesia Fisheries Management Areas (I-FMAs) 572 and 573. The catch of tuna line in I-FMA 572 and 573 reached ~30.4% of the total catch 189,022 tonnes in 2019. Limited data and scientific advice for developing adequate management remain a challenge for tuna HL-TR fisheries in I-FMA 573 waters. Center for Fisheries Research (CFR) has conducted an assessment using the PSA method to obtain information of vulnerability level both of target and bycatch species on tuna HL-TR in 2020. Data used in the analysis were grouped into operational fishing, productivity, and susceptibility data. The operational fishing data were obtained from Sadeng Fishing Port in2019. The productivity and susceptibility data were obtained from literature review and expert consultation through a Focus Group Discussion (FGD) and expert consultative workshop. The result showed that both target and by-catch species included in the analysis have low to moderate vulnerability level scores. The results of this research are used to help managers at a national level to determine necessary management measures with high precautionary. While for by-catch species, in particular white tip sharks, its management measures must comply with agreed IOTC regulations.