Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria

Denoncourt, Alix; Paquet, ValÃ©rie E.; Charette, Steve J.

doi:10.3389/fmicb.2014.00240

Cited by 66 publications

(67 citation statements)

References 104 publications

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“…pneumoniae, E. aerogenes, R. terrigena, and R. ornitholytica. Previous data demonstrated that Enterobacteriaceae can survive and multiply within amoeba host [23,[35][36][37][38][39] which is consistent with our findings. At 48h co-incubation, the intracellular counts for all bacteria in our study decreased onwards.…”

Section: Co-culture Of a Spelaea And Bacteriasupporting

confidence: 93%

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Mohamed¹,

Huseein²,

Farrag³

et al. 2016

J Bacteriol Parasitol

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Allovahlkampfia spelaea was identified for the first time in 2009. As a free living amoeba, it has been suggested to be a protective host for some bacterial pathogens against harsh environmental conditions and can transmit them to vulnerable hosts. We aimed in this study to test the interactions between Allovahlkampfia spelaea and some waterborne and foodborne bacteria and unravel if the tested bacteria can survive and multiply inside amoeba. We used a keratitis isolate of Allovahlkampfia spelaea grown in PYG medium containing proteose peptone, yeast extracts, and glucose. We examined amoeba interactions with Methicillin resistant Staphylococcus aureus, Escherichia coli 1, Klebsiella pneumoniae, Enterobacter aerogenes, Citrobacter cloaca, Proteus mirabilis, Raoultella terrigena, Raoultella ornitholytica, Aeromonas hydrophila and Pseudomonas aeruginosa using the co-culture assays. Amoebal survival rate with different bacterial strains were determined. With the exception of Proteus mirabilis that showed decreased survival rates inside amoebal cells, other bacterial isolates could survive and multiply inside Allovahlkampfia spelaea that was associated with decreased survival rates of the amoeba. Particularly, Pseudomonas aeruginosa, Aeromonas hydrophila and MRSA exhibited significantly increased multiplication rates inside amoeba. Our study demonstrated that Allovahlkampfia spelaea may act as a replicative host for pathogenic bacteria with environmental and clinical implications.

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Section: Co-culture Of a Spelaea And Bacteriasupporting

confidence: 93%

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Mohamed¹,

Huseein²,

Farrag³

et al. 2016

J Bacteriol Parasitol

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“…Without V-ATPase at the LCV, the vacuole fails to acidify, the lack of a proton gradient prevents lysosomal enzymes from functioning, and there is a lack of ability to transport ions for metal poisoning, all involved in bacterial killing. shown to be released via pellets Denoncourt et al, 2014;Faulkner et al, 2008;Gardunow et al, 2008). Post-lysosomal compartments of other protists are formed but have not been well studied (Stewart & Weisman, 1972).…”

Section: Phagotrophy By Protists Versus Phagocytosismentioning

confidence: 99%

Evasion of phagotrophic predation by protist hosts and innate immunity of metazoan hosts byLegionella pneumophila

Best

Kwaik

2018

Cellular Microbiology

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Summary Legionella pneumophila is a ubiquitous environmental bacterium that has evolved to infect and proliferate within amoebae and other protists. It is thought that accidental inhalation of contaminated water particles by humans is what has enabled this pathogen to proliferate within alveolar macrophages and cause pneumonia. However, the highly evolved macrophages are equipped more sophisticated innate defense mechanisms than protists, such as the evolution of phagotrophic feeding into phagocytosis with more evolved innate defense processes. Not surprisingly, the majority of proteins involved in phagosome biogenesis (~80%) have origins in the phagotrophy stage of evolution. There are a plethora of highly evolved cellular and innate metazoan processes, not represented in Protist biology, that are modulated by L. pneumophila; including TLR2 signaling, NF-κB, apoptotic and inflammatory processes, histone modification, caspases, and the NLRC-Naip5 inflammasomes. Importantly, L. pneumophila infects hemocytes of the invertebrate Galleria mellonella, kill G. mellonella larvae, and proliferate in and kill Drosophila adult flies and Caenorhabditis elegans. Although co-evolution with protist hosts has provided a substantial blueprint for L. pneumophila to infect macrophages, we discuss the further evolutionary aspects of co-evolution of L. pneumophila and its adaptation to modulate various highly evolved innate metazoan processes prior to becoming a human pathogen.

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“…Because the molecular basis of phagocytosis is conserved between 49 amoebae and mammals, many bacteria that can evade intracellular killing and replicate inside 50 immune phagocytes also infect and kill free-living amoebae [12,13]. Indeed, amoebae are 51 thought to be the primary reservoir for these bacterial pathogens in nature and potentially a 52 natural predator that selected for their pathogenic features [14][15][16][17][18][19][20]. 53 54 Phagocytosis is a specific process, requiring host receptors that recognize and bind ligands on 55 the bacterial cell surface [21][22][23][24][25].…”

Section: Background 33 34mentioning

confidence: 99%

Discrimination of Gram-Positive versus Gram-Negative Bacteria by the Social AmoebaDictyostelium discoideum

Rashidi

2018

Preprint

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13Professional phagocytes detect, pursue, engulf, and kill bacteria. While all professional 14 phagocytes use chemotaxis to locate bacteria, little is known about whether they can 15 discriminate among them, responding preferentially to some bacteria over others. Here we 16 examine the chemotaxis of the soil amoeba and professional phagocyte Dictyostelium 17 discoideum in assays where amoebae were presented with a paired choice of different bacteria. 18We observed variation in the extent to which they pursue different types of bacteria and 19 preferential migration towards Gram-negative over Gram-positive bacteria. Response profiles 20 were similar for amoebae isolated from different geographic locations, suggesting that 21 chemotaxis preferences are not strongly influenced by any local variation in the bacterial 22 community. Because cyclic adenosine monophosphate (cAMP) is a known chemoattractant for 23D. discoideum, we tested whether it mediated the preference for Gram-negative bacteria. 24Chemotaxis was diminished in response to a cAMP-deficient strain of Escherichia coli and 25 enhanced in response to an E. coli strain that overproduces cAMP. We conclude that D. 26 discoideum discriminates at a distance among bacteria and that discrimination is mediated in 27 part by sensing of cAMP. Preferential sensing and response to different bacteria may help to 28 explain why some amoeba-bacterial associations are more prevalent in nature than others. 29 30 31 32

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Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria

Cited by 66 publications

References 104 publications

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Evasion of phagotrophic predation by protist hosts and innate immunity of metazoan hosts byLegionella pneumophila

Discrimination of Gram-Positive versus Gram-Negative Bacteria by the Social AmoebaDictyostelium discoideum

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