Potential role of circulating microRNAs as a biomarker for unexplained recurrent spontaneous abortion

Qin, Weibing; Tang, Yunge; Yang, Ning; Wei, Xiangcai; Wu, Jie-hua

doi:10.1016/j.fertnstert.2016.01.028

Cited by 70 publications

(53 citation statements)

References 35 publications

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“…The data generated by the q-PCR assay were consistent with the microarray analyses, and the correlation-coefficient between the mean values of ten individuals generated by both techniques for each miRNA was statistically significant (Supplementary Figure S1A and Supplementary Table S1), indicating the reliability of the array data generated by miRNA microarray. In this study, many miRNAs associated with azoospermia, such as miR-199a-5p21, miR-181a22, miR-22123, miR-14119, and miR-4291924, were found to be significantly modulated by exposure to MC-LR (Table 1). Moreover, some miRNAs involved in the mechanisms of other reproductive system diseases, including the urinary tract tumor, prostate cancer, and genital tumor, were also detected25262728.…”

Section: Resultsmentioning

confidence: 60%

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Chen

Zhou

Wang

et al. 2016

Sci Rep

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Microcystin-leucine arginine (MC-LR) is a potent toxin for Sertoli cells. However, the specific molecular mechanisms of MC-induced cytotoxicity still remain unclear. In this study, we performed a comprehensive analyses of changes of miRNAs and mRNAs in Sertoli cells treated with MC-LR. Through computational approaches, we showed the pivotal roles of differentially expressed miRNAs that were associated with cell metabolism, cellular growth and proliferation, cell-to-cell signaling and interaction and cellular movement. Ingenuity Pathway Analyses (IPA) revealed some differentially expressed miRNAs and mRNAs that may cause reproductive system diseases. Target gene analyses suggested that destruction in tight junctions (TJ) and adherens junctions (AJ) in testes may be mediated by miRNAs. Consistent with a significant enrichment of chemokine signaling pathways, we observed numerous macrophages in the testes of mice following treatment with MC-LR, which may cause testicular inflammation. Moreover, miR-98-5p and miR-758 were predicted to bind the 3′-UTR region of the mitogen-activated protein kinase 11 (MAPK11, p38 β isoform) gene which stimulates tumor necrosis factor-α (TNF-α) expression in Sertoli cells. TNF-α could interact with the tumor necrosis factor receptor 1 (TNFR1) on germ cells leading to induction of germ cell apoptosis. Collectively, our integrated miRNA/mRNA analyses provided a molecular paradigm, which was experimentally validated, for understanding MC-LR-induced cytotoxicity.

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Section: Resultsmentioning

confidence: 60%

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Chen

Zhou

Wang

et al. 2016

Sci Rep

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“…Zhang et al reported that low serum miR-320b expression could act as a novel indicator of carotid atherosclerosis (29). Moreover, Qin et al provided evidence that elevating the expression of circulating miRNA-320b might serve as a biomarker for unexplained recurrent spontaneous abortion (URSA) (30). However, limited studies were focused on the role of miRNA-320b as an important regulator in tumor with its pathological role in glioma still poorly understood.…”

Section: No Of Patients --------------------------------------------mentioning

confidence: 99%

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

Liu

et al. 2017

Oncology Reports

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Accumulating evidence demonstrates that dysregulated microRNAs (miRNAs) play a critical role in tumorigenesis and progression of various cancers. miR-320b, a member of miR‑320 family, was revealed downregulated in numerous human cancers, including nasopharyngeal carcinoma and colorectal cancer. However, the function of miR‑320b in human glioma remained poorly defined. In this study, we report that miR‑320b was lowly expressed in glioma tissues and cell lines in contrast with controls, being closely correlated with histological malignancy of glioma. Furthermore, patients with low expression of miR‑320b were associated with poor prognostic outcomes. In vitro functional assays indicated that overexpression of miR‑320b could markedly enhance cell apoptosis rate and suppress cell proliferation, migration and invasion. miR-320b mimic impaired cell cycle and metastasis through inhibiting the expression of G1/S transition key regulator Cyclin D1 as well as decreasing the expression level of MMP2 and MMP9. Additionally, upregulation of miR‑320b could markedly promote apoptosis by increasing the level of Bax and reducing Bcl-2 expression in glioma. Taken together, our data suggested that miR‑320b might serve as a novel prognostic marker and potential therapeutic target for glioma.

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“…Qin et al showed that miR-559 might be involved in the pathogenesis of unexplained recurrent spontaneous abortion. 22 Chen and his colleagues revealed that miR-559 and miR-548d-3p could synergistically inhibit the expression of the oncogene ERBB2. 23 Yang et al 9 reported that miR-559 function as a tumor suppressor in glioblastoma multiform, and its inhibitory effect was related to metadherin and PTEN-AKT pathway.…”

Section: Discussionmentioning

confidence: 99%

MiR‐559 targets GP73 to suppress proliferation and invasion of hepatocellular carcinoma in vitro

Xiang

Liu

Zhang

et al. 2020

The Kaohsiung J of Med Scie

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Hepatocellular carcinoma (HCC) is one of the common malignant tumors with poor overall prognosis. As a tumor suppressor, the function of miR-559 in HCC is not clear. In this study, quantitative real-time PCR was carried out to measure the expression of miR-559 in HCC cell lines. The effects of miR-559 on HCC cell proliferation, migration, and invasion were evaluated through a series of functional assays. The mechanism through which miR-559 regulates HCC cells was investigated by dualluciferase reporter assay and functional experiments. The results revealed that miR-559 expression was low in HCC cell lines. Upregulation of miR-559 suppressed HCC cell proliferation, migration, and invasion. Dual-luciferase reporter assay confirmed Golgi membrane protein 73 (GP73) as a target gene of miR-559. Moreover, miR-559 could negatively regulate GP73 expression in HCC cells. These results demonstrated that low-level expression of miR-559 was associated with HCC, and overexpression of miR-559 could inhibit HCC cell growth and invasion via targeting GP73.

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Potential role of circulating microRNAs as a biomarker for unexplained recurrent spontaneous abortion

Abstract: Circulating microRNAs may be involved in URSA pathogenesis and provide a promising new diagnostic biomarker for URSA.

Cited by 70 publications

References 35 publications

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

MiR‐559 targets GP73 to suppress proliferation and invasion of hepatocellular carcinoma in vitro

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