Potential role of high mobility group box protein 1 and intermittent PTH (1–34) in periodontal tissue repair following orthodontic tooth movement in rats

Wolf, Michael; Lossdörfer, Stefan; Abuduwali, Nuersailike; Jäger, Andreas

doi:10.1007/s00784-012-0777-2

Cited by 27 publications

(16 citation statements)

References 46 publications

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“…In vitro and in vivo studies have shown that HMGB1 may be essential to the differentiation of osteoclast precursors by RANKL [35]. Also, HMGB1 was shown to be associated with RANKL in the regulation of host response during periodontal repair following orthodontic tooth movement in rats [36]. More studies are necessary to investigate the role of HMGB1 in osteoclastogenesis during periodontal disease to better elucidate whether this protein has important participation, whether alone or in cooperation with other osteoclastogenesis-inducing signaling molecules.…”

Section: Discussionmentioning

confidence: 99%

HMGB1 Localization during Experimental Periodontitis

Nogueira

Souza

Molon

et al. 2014

Mediators of Inflammation

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Aim. This study sought to investigate the in vitro expression profile of high mobility group box 1 (HMGB1) in murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1β and in vivo during ligature- or LPS-induced periodontitis in rats. Material and Methods. For the in vivo study, 36 rats were divided into experimental and control groups, and biopsies were harvested at 7–30 d following disease induction. Bone loss and inflammation were evaluated. HMGB1 expression was assessed by immunohistochemistry, qPCR, and Western blot. Results. Significant increases in mPDL HMGB1 mRNA occurred at 4, 8, and 12 h with protein expression elevated by 24 h. HMGB1 mRNA expression in gingival tissues was significantly increased at 15 d in the LPS-PD model and at 7 and 15 d in the ligature model. Immunohistochemical staining revealed a significant increase in the number of HMGB1-positive cells during the experimental periods. Conclusion. The results show that PDL cells produce HMGB1, which is increased and secreted extracellularly after inflammatory stimuli. In conclusion, this study demonstrates that HMGB1 may be associated with the onset and progression of periodontitis, suggesting that further studies should investigate the potential role of HMGB1 on periodontal tissue destruction.

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Section: Discussionmentioning

confidence: 99%

HMGB1 Localization during Experimental Periodontitis

Nogueira

Souza

Molon

et al. 2014

Mediators of Inflammation

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“…To analyze glycine receptor expression, tissue sections were processed for immunohistochemical detection of glycine receptor expression according to previously established protocols [22, 23]. In brief, sections were incubated with primary antibody of rabbit origin raised against a peptide mapping at the carboxy terminus of the protein (anti-glycine receptor, SYSY, Göttingen, Germany) in a 1 : 250 working solution of TBS/BSA at 4°C overnight in a humidified chamber.…”

Section: Methodsmentioning

confidence: 99%

Potential Immune Modularly Role of Glycine in Oral Gingival Inflammation

Schaumann¹,

Kraus

Winter³

et al. 2013

Clinical and Developmental Immunology

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Gingival epithelial cells (GECs) represent a physical barrier against bacteria and are involved in the processes of innate immunity. Recently, an anti-inflammatory and immune-modulatory effect of the amino acid glycine has been demonstrated. However, there is only little information about the immune-modulatory effects of glycine in oral tissues. This study aimed to investigate the existence and role of the glycine receptor in gingival tissue analyzing tissues/cells from extracted human molars via immunohistochemical analysis. In vitro, GECs were challenged by inflammatory conditions with IL-1β alone or in combination with glycine and analyzed for cytokine expression of IL6/IL8 via real-time PCR. On protein level, the effect of nuclear translocalization of NFκB protein p65 was analyzed using immunofluorescence analysis. A distinct proof of the GlyR in oral gingival tissue and keratinocytes could be demonstrated. Isolated challenge of the keratinocytes with IL-1β as well as with glycine resulted in an upregulation of IL6 and IL8 mRNA expression and activation of NFκB pathway. The presence of glycine in combination with the inflammatory stimulus led to a significant decrease in inflammatory parameters. These results indicate a possible anti-inflammatory role of glycine in gingival inflammation and encourage further research on the utility of glycine in the prevention or therapy of inflammatory periodontitis.

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“…HMGB1 induces human lung endothelial cell cytoskeletal rearrangement and barrier disruption (Wolfson et al, 2011). In addition, HMGB1 promotes periodontal remodeling and repair (Wolf et al, 2012; Wolf et al, 2013a; Wolf et al, 2013b; Wolf et al, 2013c). …”

Section: Hmgb1 Functionmentioning

confidence: 99%

HMGB1 in health and disease

Kang

Chen

Zhang

et al. 2014

Molecular Aspects of Medicine

822

828

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Complex genetic and physiological variations as well as environmental factors that drive emergence of chromosomal instability, development of unscheduled cell death, skewed differentiation, and altered metabolism are central to the pathogenesis of human diseases and disorders. Understanding the molecular bases for these processes is important for the development of new diagnostic biomarkers, and for identifying new therapeutic targets. In 1973, a group of non-histone nuclear proteins with high electrophoretic mobility was discovered and termed High-Mobility Group (HMG) proteins. The HMG proteins include three superfamilies termed HMGB, HMGN, and HMGA. High-mobility group box 1 (HMGB1), the most abundant and well-studied HMG protein, senses and coordinates the cellular stress response and plays a critical role not only inside of the cell as a DNA chaperone, chromosome guardian, autophagy sustainer, and protector from apoptotic cell death, but also outside the cell as the prototypic damage associated molecular pattern molecule (DAMP). This DAMP, in conjunction with other factors, thus has cytokine, chemokine, and growth factor activity, orchestrating the inflammatory and immune response. All of these characteristics make HMGB1 a critical molecular target in multiple human diseases including infectious diseases, ischemia, immune disorders, neurodegenerative diseases, metabolic disorders, and cancer. Indeed, a number of emergent strategies have been used to inhibit HMGB1 expression, release, and activity in vitro and in vivo. These include antibodies, peptide inhbitiors, RNAi, anti-coagulants, endogenous hormones, various chemical compounds, HMGB1-receptor and signaling pathway inhibition, artificial DNAs, physical strategies including vagus nerve stimulation and other surgical approaches. Future work further investigating the details of HMGB1 localizationtion, structure, post-translational modification, and identifccation of additional partners will undoubtedly uncover additional secrets regarding HMGB1’s multiple functions.

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Potential role of high mobility group box protein 1 and intermittent PTH (1–34) in periodontal tissue repair following orthodontic tooth movement in rats

Abstract: The findings point to the potential benefit of iPTH in the attempt to support these immune-associated reparative processes.

Cited by 27 publications

References 46 publications

HMGB1 Localization during Experimental Periodontitis

HMGB1 Localization during Experimental Periodontitis

Potential Immune Modularly Role of Glycine in Oral Gingival Inflammation

HMGB1 in health and disease

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