Potential Role of Silencing Ribonucleic Acid for Esophageal Cancer Treatment

Wu, Brian Bo-Chang; Hsu, An; Abadchi, Sanaz Nourmohammadi; Johnson, Christopher R.; Bengali, Sameer; Lay, Frank; Melinosky, Kelsey; Shao, Chunbo; Chang, Kyungho; Born, Louis J.; Abraham, John M.; Evans, David M.; Ha, Jinny S.; Harmon, John W.

doi:10.1016/j.jss.2022.04.029

Cited by 4 publications

(2 citation statements)

References 70 publications

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“…The discovery of Hypoxia-Inducible Factor-1α (HIF-1α) has suggested a new approach to the ischemic preconditioning of surgical flaps 16 , 17 . HIF-1α is a subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF-1), which coordinates the response of the cells to hypoxia and thus promotes cell survival 18 , 19 . HIF-1α enables the cellular adjustment to hypoxic conditions through the activation of particular genes associated with vascularization, angiogenesis, metabolism, and cell survival 20 .…”

Section: Introductionmentioning

confidence: 99%

Transfection of hypoxia-inducible factor-1α mRNA upregulates the expression of genes encoding angiogenic growth factors

Wlodarczyk,

Leng,

Abadchi

et al. 2024

Sci Rep

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Hypoxia-Inducible Factor-1α (HIF-1α) has presented a new direction for ischemic preconditioning of surgical flaps to promote their survival. In a previous study, we demonstrated the effectiveness of HIF-1a DNA plasmids in this application. In this study, to avoid complications associated with plasmid use, we sought to express HIF-1α through mRNA transfection and determine its biological activity by measuring the upregulation of downstream angiogenic genes. We transfected six different HIF-1a mRNAs–one predominant, three variant, and two novel mutant isoforms–into primary human dermal fibroblasts using Lipofectamine, and assessed mRNA levels using RT-qPCR. At all time points examined after transfection (3, 6, and 10 h), the levels of HIF-1α transcript were significantly higher in all HIF-1α transfected cells relative to the control (all p < 0.05, unpaired Student’s T-test). Importantly, the expression of HIF-1α transcription response genes (VEGF, ANG-1, PGF, FLT1, and EDN1) was significantly higher in the cells transfected with all isoforms than with the control at six and/or ten hours post-transfection. All isoforms were transfected successfully into human fibroblast cells, resulting in the rapid upregulation of all five downstream angiogenic targets tested. These findings support the potential use of HIF-1α mRNA for protecting ischemic dermal flaps.

show abstract

Section: Introductionmentioning

confidence: 99%

Transfection of hypoxia-inducible factor-1α mRNA upregulates the expression of genes encoding angiogenic growth factors

Wlodarczyk,

Leng,

Abadchi

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The discovery of Hypoxia-Inducible Factor-1α (HIF-1α) has suggested a new approach to the ischemic preconditioning of surgical aps (16,17). HIF-1α is a subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF-1), which coordinates the response of the cells to hypoxia and thus promotes cell survival (18, 19). It regulates the expression of more than 200 genes that encode angiogenic growth factors, including vascular endothelial growth factor (VEGF), placental growth factor (PGF), angiopoietins (ANGPT1, ANGPT2), stromal cell-derived factor-1(SDF-1), and platelet-derived growth factor B (PDGF-B) (20,21).…”

Section: Introductionmentioning

confidence: 99%

Transfection of Hypoxia-Inducible Factor-1α mRNA Upregulates the Expression of Genes Encoding Angiogenic Growth Factors

Włodarczyk

Leng

Abadchi

et al. 2023

Preprint

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View full text Add to dashboard Cite

Hypoxia-Inducible Factor-1α (HIF-1α) has presented a new direction for ischemic preconditioning of surgical flaps to promote their survival. In a previous study, we demonstrated the effectiveness of HIF-1a DNA plasmids in this application. In this study, to avoid complications associated with plasmid use, we sought to express HIF-1α through mRNA transfection and determine its biological activity by measuring the upregulation of downstream angiogenic genes. We transfected six different HIF-1a mRNAs–one predominant, three variant, and two novel mutant isoforms–into primary human dermal fibroblasts using Lipofectamine, and assessed mRNA levels using RT-qPCR. At all time points examined after transfection (3, 6, and 10 hours), the levels of HIF-1α transcript were significantly higher in all HIF-1α transfected cells relative to the control (all p < 0.05, unpaired Student’s T-test). Importantly, the expression of HIF-1α transcription response genes (VEGF, ANG-1, PGF, FLT1, and EDN1) was significantly higher in the cells transfected with all isoforms than with the control at six and/or ten hours post-transfection. All isoforms were transfected successfully into human fibroblast cells, resulting in the rapid upregulation of all five downstream angiogenic targets tested. These findings support the potential use of HIF-1α mRNA for protecting ischemic dermal flaps.

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Mechanisms of Luteolin Against Gastro-Esophageal Reflux Disease Based on Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

Wang,

Yan,

Wang

et al. 2024

Cell Biochem Biophys

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Potential Role of Silencing Ribonucleic Acid for Esophageal Cancer Treatment

Cited by 4 publications

References 70 publications

Transfection of hypoxia-inducible factor-1α mRNA upregulates the expression of genes encoding angiogenic growth factors

Transfection of hypoxia-inducible factor-1α mRNA upregulates the expression of genes encoding angiogenic growth factors

Transfection of Hypoxia-Inducible Factor-1α mRNA Upregulates the Expression of Genes Encoding Angiogenic Growth Factors

Mechanisms of Luteolin Against Gastro-Esophageal Reflux Disease Based on Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

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