Potential role of surface wettability on the long-term stability of dentin bonds after surface biomodification

Abstract

Degradation of the adhesive interface contributes to the failure of resin composite restorations. The hydrophilicity of the dentin matrix during and after bonding procedures may result in an adhesive interface that is more prone to degradation over time. This study assessed the effect of chemical modification of dentin matrix on the wettability and the long-term reduced modulus of elasticity (Er) of the adhesive interfaces. Human molars were divided into groups according to the priming solutions: distilled water (control), 6.5% Proanthocyanidin-rich grape seed extract (PACs), 5.75% 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/1.4% n-hydroxysuccinimide (EDC/NHS) and 5% Glutaraldehyde (GA). The water-surface contact angle was verified before and after chemical modification of the dentin matrix. The demineralized dentin surface was treated with the priming solutions and restored with One Step Plus (OS) and Single Bond Plus (SB) and resin composite. The Er of the adhesive, hybrid layer and underlying dentin was evaluated after 24 h and 30 months in artificial saliva. The dentin hydrophilicity significantly decreased after application of the priming solutions. Aging significantly decreased the Er in the hybrid layer and underlying dentin of control groups. The Er of GA groups remained stable over time at the hybrid layer and underlying dentin. Significant higher Er was observed for PACs and EDC/NHS groups at the hybrid layer after 24 h. The decreased hydrophilicity of the modified dentin matrix likely influence the immediate mechanical properties of the hybrid layer. Dentin biomodification prevented substantial aging at the hybrid layer and underlying dentin after 30 months storage.

Absolute Configuration of Native Oligomeric Proanthocyanidins with Dentin Biomodification Potency

“…12,34 The beams were tested immediately and after 6 and 12 months storage in simulated body fluid. 35 …”

Bond strength and micromorphology of resin-dentin interface of etch-and-rinse dentin bonding agents after 1-year of water storage

“…h Representative SEM micrograph of the resin-dentin interface formed by the Peak Universal Bond two-step etch-and-rinse (×2000 magnification). Samples were storage in water for 1 year (CR composite resin, HL hybrid layer, RT resin tag) degradation, increasing the longevity of bonding, as observed in this study [15,16]. No bond strength reduction was observed for Gluma Comfort Bond.…”

Quercetin as a simple but versatile primer in dentin bonding

“…It was reported that lower water in dentin surface could facilitate the inltration and polymerization of the adhesives, thereby increase the mechanical properties of the adhesive-dentin interfaces. 44 Because the solvent used to dissolve quercetin in the present study was 100% ethanol, the quercetin/ethanol primer could be viewed as a combined application of collagen cross-linker and ethanolwet bonding technique, synergistically improve the bonding stability.…”