Potential targets of transforming growth factor-beta1 during inhibition of oocyte maturation in zebrafish

Kohli, Gurneet; Clelland, Eric; Peng, Chun

doi:10.1186/1477-7827-3-53

Cited by 61 publications

(13 citation statements)

References 40 publications

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“…For example, double conditional knockout of Smad2 and Smad3, which are two TGFβ major signaling components, leads to severe fertility and fecundity defects in mice, supporting the involvement of an intraovarian Smad2/3 pathway in mediating oocyte-produced signals essential for coordinating key events of the ovulatory process (Pangas and Matzuk, 2004). Similarly, recent findings suggest that TGFβ may play a role in preventing premature oocyte maturation in zebrafish by down-regulating basal and human chorionic gonadotropin-induced 20β-hydroxysteroid dehydrogenase, the luteinizing hormone receptor, and membrane progestin receptor-β mRNA levels (Kohli et al, 2005). Our results have shown that the kinase activity of TβRII is required for zkm23 phosphorylation, and that blockade of zkm23 results in an inhibition of transcriptional activation induced by TGFβ, suggesting that zkm23 is an important TGFβ signaling intermediate in zOFCs.…”

Section: Discussionmentioning

confidence: 99%

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Requirement of a dynein light chain in transforming growth factor β signaling in zebrafish ovarian follicle cells

Jin

Gao

Mulder

2012

Molecular and Cellular Endocrinology

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We have previously reported that the dynein light chains km23-1 and km23-2 are required for TGFβ signaling in mammalian cells. Here we describe another member of the km23/DYNLRB/LC7/robl family of dynein light chains in zebrafish, termed zkm23, which is also involved in TGFβ signaling. zkm23 was rapidly phosphorylated after TGFβ stimulation. TGFβ RII kinase activity was absolutely required for zkm23 phosphorylation, whereas a constitutively active TGFβ RI did not induce phosphorylation. Further, TGFβ stimulated a rapid recruitment of the zkm23 dynein light chain to the dynein intermediate chain of the dynein complex, and the TGFβ RII kinase was required for this interaction. Finally, blockade of zkm23 using morpholino oligos resulted in an inhibition of TGFβ-mediated transcriptional responses. Thus, our results demonstrate for the first time that the dynein light chain zkm23 is required for TGFβ signaling in cultured zebrafish ovarian follicle cells.

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Section: Discussionmentioning

confidence: 99%

“…In addition, there is evidence for the presence of the major TGFβ signaling pathway molecules in the fish ovary (Calp et al, 2003; Dick et al, 2000; Hardie et al, 1998; Kohli et al, 2005; Kohli et al, 2003). Further, TGFβ sumperfamily members (TGFβ, Activin, Inhibin, GDF9, etc.)…”

Section: Introductionmentioning

confidence: 99%

Requirement of a dynein light chain in transforming growth factor β signaling in zebrafish ovarian follicle cells

Jin

Gao

Mulder

2012

Molecular and Cellular Endocrinology

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“…In human, miR-202-5p levels in follicular fluid are positively correlated with the fertilization potential of oocytes (41), suggesting a role for miR-202-5p in the proper development of oocytes. Moreover, miR-202-5p has been shown to regulate PI3K (42) and TGF-β signaling, both of which play important roles in folliculogenesis and oocyte maturation (43, 44).…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel MicroRNAs and Characterization of MicroRNA Expression Profiles in Zebrafish Ovarian Follicular Cells

Zayed

Peng

2019

Front. Endocrinol.

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MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression primarily at the post-transcriptional levels and thereby play important roles in regulating many physiological and developmental processes. Oocyte maturation in fish is induced by hormones produced from the hypothalamus, pituitary, and ovary. Gonadotropin-releasing hormone (GnRH) stimulates the secretion of luteinizing hormone (LH), which in turn, induces the secretion of maturation-inducing hormone (MIH) from the ovary. It is documented that small early vitellogenic (or stage IIIa) follicles are unable to undergo oocyte maturation whereas oocytes in mid- to late vitellogenic (stage IIIb) follicles can be induced by LH and MIH to become mature. To determine whether miRNAs may be involved in the growth and acquisition of maturational competency of ovarian follicles, we determined the miRNA expression profiles in follicular cells collected from stage IIIa and IIIb follicles using next-generation sequencing. It was found that miRNAs are abundantly expressed in the follicular cells from both stages IIIa and IIIb follicles. Furthermore, bioinformatics analysis revealed the presence of 214 known, 31 conserved novel and 44 novel miRNAs in zebrafish vitellogenic ovarian follicular cells. Most mature miRNAs in follicular cells were found to be in the length of 22 nucleotides. Differential expression analysis revealed that 11 miRNAs were significantly up-regulated, and 13 miRNAs were significantly down-regulated in the stage IIIb follicular cells as compared with stage IIIa follicular cells. The expression of four of the significantly regulated miRNAs, dre-miR-22a-3p, dre-miR-16a, dre-miR-181a-3p, and dre-miR-29a, was validated by real-time PCR. Finally, gene enrichment and pathway analyses of the predicted targets of the significantly regulated miRNAs supported the involvement of several key signaling pathways in regulating ovarian function, including oocyte maturation. Taken together, this study identifies novel zebrafish miRNAs and characterizes miRNA expression profiles in somatic cells within the zebrafish ovarian follicles. The differential expression of miRNAs between stage IIIa and IIIb follicular cells suggests that these miRNAs are important regulators of zebrafish ovarian follicle development and/or oocyte maturation.

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“…Although in lower vertebrates there is no direct evidence to firmly establish a functional similarity between fish and mammalian TGF-s, experimental data suggest that fish TGF-s have some functional roles similar to those observed in mammals. TGF-has been observed to influence both reproduction and immune response: in the zebrafish Danio rerio TGF-inhibits gonadotropin-and 17alpha, 20beta-dihydroxyprogesterone-induced oocyte maturation [54]. In the rainbow trout Oncorhincus mykiss, treatment with mammalian TGF-1 significantly inhibits macrophage respiratory burst activity [55] and production of macrophage activating factors by head kidney leucocytes [56].…”

Section: Tgf-mentioning

confidence: 98%

Growth Factors and Chemokines: A Comparative Functional Approach Between Invertebrates and Vertebrates

Tettamanti¹,

Malagoli²,

Benelli³

et al. 2006

CMC

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Growth factors and cytokines control and coordinate a broad spectrum of fundamental cellular functions, and are evolutionarily conserved both in vertebrates and invertebrates. In this review, we focus our attention on the functional phylogenetic aspects of growth factors/cytokines like the Transforming Growth Factor-beta (TGF-beta), the Connective Tissue Growth Factor (CTGF), and the Vascular Endothelial Growth Factor (VEGF). We will also delve into the activites of two chemokine families, interleukin (IL)-8 (or CXCL8) and CC chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2). These molecules have been selected for their involvement in immune responses and wound healing processes, where they mediate and finely regulate various regeneration processes like angiogenesis or fibroplasia, not only in vertebrates, but also in invertebrates.

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Potential targets of transforming growth factor-beta1 during inhibition of oocyte maturation in zebrafish

Cited by 61 publications

References 40 publications

Requirement of a dynein light chain in transforming growth factor β signaling in zebrafish ovarian follicle cells

Requirement of a dynein light chain in transforming growth factor β signaling in zebrafish ovarian follicle cells

Identification of Novel MicroRNAs and Characterization of MicroRNA Expression Profiles in Zebrafish Ovarian Follicular Cells

Growth Factors and Chemokines: A Comparative Functional Approach Between Invertebrates and Vertebrates

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