Potential toxicity of flubendiamide in<i>Drosophila melanogaster</i>and associated structural alterations of its compound eye

Sarkar, Saurabh; Dutta, Moumita; Roy, Sumedha

doi:10.1080/02772248.2014.997986

Cited by 21 publications

(14 citation statements)

References 23 publications

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“…Fahmy and Dahi () recorded a significant increase in AChE activity by 4.94 and 18.65% in spinetoram‐treated S. littoralis larvae of Kalyobia and Behiara, respectively. In contrast, Drosophila melanogaster exposed to the flubendiamide exhibited dose‐dependent inhibition trend in the activity of AChE (Sarkar, Dutta, & Roy, ). Time‐dependent response of proteases at LD 50 concentration (0.72 μM) for 72 h exposure indicated that trypsin, chymotrypsin, elastase, and total protease were significantly inhibited, indicating that flubendiamide induced antifeedant activity through inhibition of proteases.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of flubendiamide‐induced mitochondrial dysfunction and metabolic changes in Helicoverpa armigera (Hubner)

Nareshkumar

Akbar

Sharma

et al. 2017

Arch Insect Biochem Physiol

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Phthalic acid diamide insecticides are the most effective insecticides used against most of the lepidopteran pests including Helicoverpa armigera, a polyphagous pest posing threat to several crops worldwide. The present studies were undertaken to understand different target sites and their interaction with insect ryanodine receptors (RyR). Bioassays indicated that flubendiamide inhibited the larval growth in dose-dependent manner with LD value of 0.72 μM, and at 0.8 μM larval growth decreased by about 88%. Flubendiamide accelerated the Ca -ATPase activity in dose-dependent trend, and at 0.8 μM, the activity was increased by 77.47%. Flubendiamide impedes mitochondrial function by interfering with complex I and F F -ATPase activity, and at 0.8 μM the inhibition was found to be about 92% and 50%, respectively. In vitro incubation of larval mitochondria with flubendiamide induced the efflux of cytochrome c, indicating the mitochondrial toxicity of the insecticide. Flubendiamide inhibited lactate dehydrogenase and the accumulation of H O , thereby preventing the cells from lipid peroxidation compared to control larvae. At 0.8 μM the LDH, H O content and lipid peroxidation was inhibited by 98.44, 70.81, and 70.81%, respectively. Cytochrome P450, general esterases, AChE, and antioxidant enzymes (catalase and superoxide dismutase) exhibited a dose-dependent increasing trend, whereas alkaline phosphatase and the midgut proteases, except amino peptidase, exhibited dose-dependent inhibition in insecticide-fed larvae. The results suggest that flubendiamide induced the harmful effects on the growth and development of H. armigera larvae by inducing mitochondrial dysfunction and inhibition of midgut proteases, along with its interaction with RyR.

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Section: Discussionmentioning

confidence: 99%

Evaluation of flubendiamide‐induced mitochondrial dysfunction and metabolic changes in Helicoverpa armigera (Hubner)

Nareshkumar

Akbar

Sharma

et al. 2017

Arch Insect Biochem Physiol

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“…Recent years, formulating a new class of chemical with a potential for acting on specific pest posing no harm to non-targeted organism was of prime importance. One such Phthalic acid diamide class of pesticide flubendiamide (C 23 H 22 F 7 IN 2 O 4 S) was reported to be effective against a broad spectrum of lepidopteran insect pest with a relatively low toxicity to non-targeted organisms (Sarkar et al 2014 ). This formulation works by disrupting ion channels and thereby paralysing the muscle fibres of the gut (Nishimatsu et al 2005 ), therefore, the larvae stops feeding and eventually dies from starvation (Ebbinghaus-Kintscher et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

“…Even though precisely targeted formulations of flubendiamide are expected to be safe for non-target organisms, yet, several recent studies have shown toxic potentials of flubendiamide on many non-target organisms. Neurotoxic potentials and morphological anomalies in drosophila melanogaster have been reported (Sarkar et al 2014 ). Acute and joint toxicity of flubendiamide was reported by Wei et al ( 2014 ) on Chinese tiger frog Hoplobatrachus chinensis tadpoles.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of flubendiamide by a newly isolated Chryseobacterium sp. strain SSJ1

Jadhav

David

2016

3 Biotech

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Flubendiamide, as a new class (Phthalic acid diamide) of pesticide with a wide spectrum of activity against lepidopteran pests extensively used alone or in combination with other insecticides in agriculture system to get protection from insect pests. Due to high specificity and limited approach towards non-target organism, the extensive use of this pesticide as an alternate for organophosphate and organochlorine pesticides, causing an eventual increase in environmental pollution. Five flubendiamide-resistant bacterial strains were isolated during the present study from agriculture soil considering previous history of pesticide application. Minimal inhibitory concentration of all the isolates showed strain SSJ1 was most efficient flubendiamide resistant organism. Biochemical tests and molecular sequencing of 16s rRNA was carried out which confirmed the isolate as Chryseobacterium indologenes strain SSJ1. UV–visible spectrophotometer study revealed that 89.06 % initial pesticide was removed by the isolate at optimum temperature of 35 °C and pH 7.0 with 5 days incubation period and is further confirmed by high-performance liquid chromatography (HPLC) analysis. Results of the present study however, suggest strain SSJ1 is most resistant to flubendiamide and can possibly be applied in the bioremediation of flubendiamide contaminated soils.

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“…Since the formulation is targeted against lepidopteran insects, cross-reactivity leading to hazardous impact in non-target Dipterans, like Drosophila melanogaster Meigen (Diptera: Drosophilidae) is quite unanticipated. In our previous studies, the neurotoxic potential of this chemical against Drosophila melanogaster Meigen (Diptera: Drosophilidae) was discussed (Sarkar et al ., 2015a ). In the present work we assessed the effects of Flubendiamide at Indian field doses on the compound eye morphology of D. melanogaster, a very accessible model organism (Rand, 2010 ; Sarkar et al , 2017a ).…”

Section: Introductionmentioning

confidence: 99%

“…More specifically, the compound eye of D. melanogaster is yet again an established model used as an index for toxicity in environmental monitoring studies, where any possible alteration in its architecture would indicate the risk of exposure. Several studies on chemically induced alteration in compound eye have reported such variation in eye morphology (Podder et al ., 2012 ; Dutta et al ., 2014a ; Sarkar et al ., 2015a ). The study further explores the possibilities of trans-generational transfer of Flubendiamide induced alterations in the compound eye architecture for four (P, F 1 , F 2 and F 3 ) consecutive generations, similar to the findings of NaF exposure in Drosophila (Dutta et al ., 2014b ; Yiamouyiannis, 1983 ).…”

Section: Introductionmentioning

confidence: 99%

Flubendiamide induces transgenerational compound eye alterations in Drosophila melanogaster

Sarkar

Roy

2017

Interdisciplinary Toxicology

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Pesticides are one of the major sources of environmental toxicity and contamination. This study reports potential of lepidopteran insecticide formulation, named Flubendiamide, in altering compound eye architecture and bristle pattern orientation for four consecutive generations (P, F1, F2 and F3) in a non-target diptera, Drosophila melanogaster Meigen (Diptera: Drosophilidae). The concentrations of the insecticide formulation selected for treatment of Drosophila (50 and 100 μg/mL) were in accordance with practiced Indian field doses (50 μg/mL for rice and 100 μg/mL for cotton). This study showed trans-generational insecticide-induced changes in the morphology of the compound eyes of the non-target insect D. melanogaster.

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Potential toxicity of flubendiamide inDrosophila melanogasterand associated structural alterations of its compound eye

Cited by 21 publications

References 23 publications

Evaluation of flubendiamide‐induced mitochondrial dysfunction and metabolic changes in Helicoverpa armigera (Hubner)

Evaluation of flubendiamide‐induced mitochondrial dysfunction and metabolic changes in Helicoverpa armigera (Hubner)

Biodegradation of flubendiamide by a newly isolated Chryseobacterium sp. strain SSJ1

Flubendiamide induces transgenerational compound eye alterations in Drosophila melanogaster

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