Potential Use of Mosquito’s Salivary Components as Novel Target for The Development of Transmission Blocking Vaccine (TBV)

Senjarini, Kartika

doi:10.5454/mi.7.4.7

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…darlingi (Fontaine et al, 2012), An. barbirostris (Phattanawiboon et al, 2014) (Senjarini, 2013;Armiyanti, 2015), sedangkan untuk dengue, penelitian dilakukan pada vektor utama yaitu Aedes aegypti (Oktarianti, 2015). Sementara An.…”

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Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis

Febriyantiningsih¹,

Senjarini²,

Oktarianti³

2017

J. I. Dasar

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Malaria has been prevalent for a long time in tropical developing regions causing great morbidity and mortality. Among the malaria vectors, Anopheles vagus has been known as secondary malaria vector in East Java. Salivary glands of mosquitoes perform various functions for survival of the vectors and also conducive for blood feeding, harbouring of malaria parasites, and eventual parasite transmission. The salivary gland proteomes of An. vagus have not been carried out yet. The aim of our study was to identify and characterize the immunogenic proteins of salivary glands proteins of An. vagus. A proteomic approach combining one-dimensional electrophoresis (1DE) followed by western blot analysis using human sera from healthy people living in an endemic area (Kendal); liquid chromatography mass spectrometry (LC-MS/MS) and bioinformatic analysis was adopted to provide the first direct insight into identification and characterization of salivary proteins of An. vagus. Identification of immunogenic proteins using western blot analysis has revealed three immunogenic bands which had molecular weights of 69, 75 and 232 kDa. Among those proteins analysed by LC-MS/MS, there were alpha,1-4 glucan phosphorylase, putative myosin class I heavy chain which have the highest number of total spectrum count peptide. Other proteins like vitellogenin and heat shock protein 82 (Hsp82) were also identified. The majority of proteins were scrutinized marked for their role in metabolism, cytoskeleton protein and stress response.

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Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis

Febriyantiningsih¹,

Senjarini²,

Oktarianti³

2017

J. I. Dasar

Self Cite

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Proteomic analysis of immunogenic proteins from salivary glands of Aedes aegypti

Oktarianti

Senjarini

Hayano

et al. 2015

Journal of Infection and Public Health

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Humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. This reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. Our previous study identified two immunogenic proteins with molecular weights of 31 kDa and 56 kDa from salivary gland extract (SGE) of Aedes aegypti that cross-reacted with serum samples from Dengue Hemorrhagic Fever (DHF) patients and healthy people in an endemic area (Indonesia). Serum samples from individuals living in non-endemic area (sub-tropical country) and infants did not show the immunogenic reactions. The objective of this research was to identify two immunogenic proteins, i.e., 31 and 56 kDa by using proteomic analysis. In this study, proteomic analysis resulted in identification of 13 proteins and 7 proteins from the 31 kDa- and 56 kDa-immunogenic protein bands, respectively. Among those proteins, the D7 protein (Arthropode Odorant-Binding Protein, AOBP) was the most abundant in 31-kDa band, and apyrase was the major protein of the 56-kDa band.

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Molecular Characterization of Secreted Proteins from Salivary Gland Immunogenic Protein of Anopheles vagus

Senjarini

Wathon

Febriyantiningsih

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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Salivary mosquito’s proteins have been widely acknowledged to contain factors important for pathogen transmission as well as for biomarker of Anopheles exposure. The objective of this study was to identify and characterize the secreted proteins from salivary glands and immunogenic proteins from of Anopheles (An.) vagus. A proteomic approach combining one-dimensional electrophoresis (1DE) was operationalized, followed by western blot analysis using human sera from healthy people living in an endemic area (Kendal, East Java - Indonesia); liquid chromatography mass spectrometry (LC-MS/MS) and bioinformatics analysis were conducted to gain direct insight into An. vagus salivary proteins. Three immunogenic proteins with molecular weight of 69, 75 and 232 kDa were identified. Apart from the housekeeping proteins identified by LC-MS/MS, there were also some proteins which played crucial role in the blood feeding process i.e AGA 5’ nucleotidase family, for 69 & 75 kDa and SGS 4 for 232 kDa respectively. The other known proteins like vitellogenin, putative myosin class I heavy chain and heat shock protein 70 (Hsp70) were also identified. The majority of proteins were clearly characterized in Anopheles for their role in blood feeding, metabolism, cytoskeleton protein. and stress response.

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Potential Use of Mosquito’s Salivary Components as Novel Target for The Development of Transmission Blocking Vaccine (TBV)

Cited by 3 publications

References 15 publications

Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis

Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis

Proteomic analysis of immunogenic proteins from salivary glands of Aedes aegypti

Molecular Characterization of Secreted Proteins from Salivary Gland Immunogenic Protein of Anopheles vagus

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