Humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. This reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. Our previous study identified two immunogenic proteins with molecular weights of 31 kDa and 56 kDa from salivary gland extract (SGE) of Aedes aegypti that cross-reacted with serum samples from Dengue Hemorrhagic Fever (DHF) patients and healthy people in an endemic area (Indonesia). Serum samples from individuals living in non-endemic area (sub-tropical country) and infants did not show the immunogenic reactions. The objective of this research was to identify two immunogenic proteins, i.e., 31 and 56 kDa by using proteomic analysis. In this study, proteomic analysis resulted in identification of 13 proteins and 7 proteins from the 31 kDa- and 56 kDa-immunogenic protein bands, respectively. Among those proteins, the D7 protein (Arthropode Odorant-Binding Protein, AOBP) was the most abundant in 31-kDa band, and apyrase was the major protein of the 56-kDa band.

No abstract

No abstract

Kabupaten Banyuwangi merupakan salah satu daerah endemis malaria, khususnya Desa Bangsring Kecamatan Wongsorejo. Berdasarkan laporan survei entomologi, di desa tersebut terdapat lagun yang digunakan sebagai tempat perindukan Anopheles. Penelitian ini bertujuan untuk mengamati beberapa karakteristik bionomik yang penting yaitu identifikasi spesies serta perilaku dan preferensi menghisap darah. Sampling dilakukan pada bulan Mei sampai bulan November2015, pada minggu ke 2 setiap bulannya. Penangkapan nyamuk dilakukan pada Manusia Dalam Rumah dan Manusia Luar Rumah di dua rumah berbeda. Masing-masing penangkapan setiap 40 menit, dimulai pukul 18.00 sampai 06.00. Penangkapan nyamuk yang Istirahat Dalam Rumah (IDR) dan Istirahat di Sekitar Kandang Ternak (ISKT) setiap 10 menit pada waktu yang sama. Hasil identifikasi menunjukkan spesies Anopheles yang dominan di lokasi penelitian adalah An. sundaicus. Beberapa spesies lainnya yang ditemukan diantaranya adalah An. vagus, An. subpictus, An. barbirostris dan An. indefinitus. Aktivitas mengigit Anopheles mengalami puncak kepadatan antara pukul 21.00 – 22.00. Sementara itu preferensi mengigitnya lebih bersifat eksofagik dan zoofilik. Hal ini dapat merupakan penyebab menurunnya kasus malaria di daerah tersebut selama 3 tahun terakhir semenjak terjadinya kejadian luar biasa malaria pada tahun 2011 dengan jumlah kasus sebanyak 107 kasus malaria.

Bacteria are the most dominant group of microorganisms in aquatic environments due to their role in organic matter decomposition. Decomposition activity is related to the type and dominance of bacteria in the communities. Therefore, study of bacterial diversity is an important step to understand their role in aquatic ecosystems. This study was to determine bacterial diversity and their physiological characters of bacteria from Bandealit Coast in Jember East Java Indonesia. The bacteria were confirmed by BOX-PCR profile for their genetic polymorphisms. Identification of potential isolate was conducted based on 16S rRNA gene sequence. The result showed that BA011109 isolate was able to utilize D-cellobiose as a sole substrate, indicating its ability to hydrolyse β β β β βglucoside bond. This isolate was a potential decomposer in the area considering that most of organic pollutants were from plants that cointain high cellulose. Based on its 16S rRNA gene sequence, this isolate was closely related to Microbacterium esteraromaticum with 100% homology. Further study on quantitative hydrolytic activities is needed to elucidate its role as an organic matter decomposer in aquatic environment.