Physiological and Molecular Characteristics of Bacterial Isolates from Bandealit Coastal Area Jember, East Java, Indonesia

Fitriyah, Dina; Arimurti, Sattya; Senjarini, Kartika

doi:10.4308/hjb.20.2.89

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…The microbial diversity research is of great value for many aspects, which includes 1) the development of biological resources, 2) accelerating the discovery process of new functional genes and bioactive substances, 3) the clari cation of the relationship between microbial communities and habitat, 4) the elucidation of the association between the community structure and function 15 . Previous studies have suggested that bacteria are the most dominant group of microorganisms in this environment 7 . Bacterial communities play an important role in material transformation in mangrove ecosystems 16 .…”

Section: Discussionmentioning

confidence: 90%

“…Microbes play an important role in material circulation, energy ow, and ecosystem stability 6 . In recent years, microorganisms as important members of mangrove ecosystems have received increasing attention 7,8 . Microbial diversity is an important part of biodiversity, and understanding the microbial diversity is the basis of microbial research in mangrove wetlands, which is of great value for developing microbial resources.…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of bacterial community diversity between soil and water of Dongzhai Harbor Mangrove reserve using 16S rRNA gene sequencing and shotgun metagenomic sequencing

Rong¹,

Zhang²,

Xu³

et al. 2020

Preprint

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The mangrove ecosystem has rich biological resources and is the fourth highest service value ecosystem in the world. The Dongzhai Harbor Mangroves (DHM) reserve is located in the intertidal zone where the freshwater and seawater are dynamically interlaced, and its unique habitat may hide special microbial resources. In this study, we analyzed and compared bacterial community composition and diversity between the DHM soil and water by 16S rRNA gene sequencing and shotgun metagenomic sequencing. We found that the dominant species in both soil and water of DHM were Proteobacteria and Actinobacteria, while the most differentially abundant species were Chloroflexi and Bacteroidetes. However, shotgun metagenomic sequencing analysis identified more highly abundant species in the water than in the soil, and identified more species with significant differences between the soil and the water (P ≤ 0.0001). Network analysis identified more co-occurring species in the soil and water. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analysis identified three relatively abundant pathways: metabolism (accounting for more than 50%), genetic information processing, and environment information processing. Our results increase our understanding of bacterial community diversity in the water and soil of the DHM. Additional information that is hidden in the environment may be obtained by shotgun metagenomic sequencing; this technique can be used to mine more microbial resources from various environments.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of bacterial community diversity between soil and water of Dongzhai Harbor Mangrove reserve using 16S rRNA gene sequencing and shotgun metagenomic sequencing

Rong¹,

Zhang²,

Xu³

et al. 2020

Preprint

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“…Strain I is known to be round, has a flat edge with convex elevation and wet consistency, and this strain is yellow, whereas Strain II is known to have the same shape as Strain 1, which is round but has uneven edges, flat elevation, and dry and creamy consistency. Bacteria have an essential role in the wastewater bioremediation process through their decomposition activity, where decomposition activity is highly related to the type of dominant bacteria in the communities (Fitriyah et al 2013). Morphological identification techniques conducted in order to identify poorly better described, rarely isolated, or phenotypically irregular strains (Tshikhudo et al 2013).…”

Section: Resultsmentioning

confidence: 99%

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2021

JSM

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Wastewater is the remainder of an activity released in the liquid form. Wastewater product is feared to have negative influence on the environmental balance; therefore, it is necessary to measure the quality standards of wastewater as a reference in the disposal and treatment. Bioremediation is an environmentally friendly technology utilizing microorganisms as agents in the process of cleaning or restoring wastewater conditions. The use of microorganism services can reduce the concentration of organic waste into simpler organic compounds by converting organic compounds into CO 2 , CH 4 , H 2 , and H 2 S, as well as water and energy intended for the process of growth and production of microorganisms in the remediation process. This study aims to identify the type of dominant bacteria growing in the wastewater of the sugar factory and has a potential role as a bioremediation agent for the waste. The method used in this study is the experimental, by observing several environmental parameters as indicators, among others, BOD, COD, TSS, and pH. The results of this study found two types of dominant bacteria, i.e., Staphylococcus aureus and Bacillus subtilis, then used as bioremediation agents. The bioremediation activity was able to reduce pH, BOD, and COD levels.

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“…Identification of bateria based on 16S rRNA gene suquence analysis. The whole genome DNA of this isolate was extracted using the freezing and thawing method (Fitriyah et al, 2013). Amplification of the 16S rRNA encoder used a using primers 27f (5'-GAGAGTTTGA TCCTGGCTCAG-3') and 1495r (5'-CTAC GGCTACCTTGT TACGA-3') (Arimurti et al, 2017).…”

Section: Morphology Characteristics and Identification Of Bacteriamentioning

confidence: 99%

Characterization and Identification of Caffeine-Degrading Bacteria KAJ 36

Arimurti

Afifuddin

Siswanto

et al. 2020

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Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. These bacteria have the potential as an agent of decaffeinating coffee. The objective of this research was to characterize and identify the caffeinedegrading bacteria KAJ 36 that was isolated from the pulp waste of Coffea arabica. The isolate-characterization was performed based on its growth and caffeine degradation activity.in a medium M9 added with 10 g/L caffeine for 7 days. The isolate identification was conducted using 16S rRNA sequence analysis and biochemical analysis. The result indicated that caffein-degrading bacteria were able to grow and degrade the caffein up to 86% activity on M9 medium added 10 g/L caffeine for 7 days incubation. Based on 16S rRNA, the isolated KAJ 36 had sequences of 99% homology with Pseudomonas monteilii. Biochemical analysis showed that this isolation on the specific medium was a gram-negative, oxidative, positive enzymatic activity (oxidase, catalase, urease) and reduce nitrate. Our results showed that the isolated KAJ 36 was capable of producing fluorescent pigment. This bacterium was unable to produce indole or utilize sucrose and lactose. Based on the cultural characteristics, we found that the isolated KAJ 36 could be grown in a medium with pH 5, 7, and 9, and in the environment with up to 37oC. This evidence suggested that P. monteilii KAJ 36 can be used as a potential degradation agent of caffeine and recommend for future research in the bacterial based on the degradation of caffeine-contained coffee.

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Physiological and Molecular Characteristics of Bacterial Isolates from Bandealit Coastal Area Jember, East Java, Indonesia

Cited by 7 publications

References 10 publications

Comparative analysis of bacterial community diversity between soil and water of Dongzhai Harbor Mangrove reserve using 16S rRNA gene sequencing and shotgun metagenomic sequencing

Comparative analysis of bacterial community diversity between soil and water of Dongzhai Harbor Mangrove reserve using 16S rRNA gene sequencing and shotgun metagenomic sequencing

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Characterization and Identification of Caffeine-Degrading Bacteria KAJ 36

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